sort by

10 publications mentioning bmo-mir-275

Open access articles that are associated with the species Bombyx mori and mention the gene name mir-275. Click the [+] symbols to view sentences that include the gene name, or the word cloud on the right for a summary.

1
[+] score: 105
Other miRNAs from this paper: bmo-mir-9a, bmo-mir-2b, bmo-mir-1926, bmo-mir-965, bmo-mir-2739
From the above results, we conclude that BmSer-2is one of the target genes of bmo-miR-275, and that bmo-miR-275 can down-regulate the expression of the BmSer-2 gene by interacting with the 3′UTR of its mRNA in vitro. [score:8]
In this study, we performed bioinformatics analysis to identify potential targets of bmo-miR-275 and subsequently analyzed the role of bmo-miR-275 in the regulation of BmSer-2. Our data provide important insights into miRNA functions in B. mori and the molecular mechanisms regulating the expression of genes encoding silk proteins. [score:7]
BmN cells were co -transfected with the bmo-miR-275 recombinant expression vector and the recombinant expression vector containing the 3′UTR sequence of the target gene. [score:7]
Temporal and spatial characteristics of the expression of bmo-miR-275 and its target gene in BmSer-2The expression levels of bmo-miR-275 and its target gene BmSer-2 in different tissues and organs of the fifth instar, 3-day larvae were detected using semi-quantitative reverse transcription polymerase chain reaction (RT-PCR)(The primers are list in Table 1). [score:6]
Plasmid transfection for verification of the regulation of predicted target gene expression by bmo-miR-275. [score:6]
The expression vector pcDNA3.0 [ie1-egfp-pri-bmo-miR-275-SV40] for miR-275 expression was successfully constructed to contain the BmNPV ie1 promoter and an egfp reporter gene. [score:5]
Construction of recombinant vectors expressing bmo-miR-275 and its predicted target genes. [score:5]
Regulation of BmSer-2 expression by bmo-miR-275. [score:4]
The expression levels of bmo-miR-275 and its target gene BmSer-2 in different tissues and organs of the fifth instar, 3-day larvae were detected using semi-quantitative reverse transcription polymerase chain reaction (RT-PCR)(The primers are list in Table 1). [score:4]
In contrast, bmo-miR-275 was expressed only in the middle silk gland (Fig 3). [score:3]
These results suggest that the expression of bmo-miR-275 and BmSer-2 in the fifth instar 3-day larvae was highly tissue specific. [score:3]
Effect of bmo-miR-275 expressionon luciferase activity in transfected BmN cells. [score:3]
0190464.g003 Fig 3Expression analyses of bmo-miR-275, BmSer-2, and U6 in different tissues of 5th instar,3-day larvae of B. mori. [score:3]
Prediction of bmo-miR-275 targeting BmSer-2 3’UTR. [score:3]
cgi) were cloned into the vector with BmNPV ie1 as the promoter to construct the recombinant vector expressing bmo-miR-275 pcDNA3 (ie1-egfp-pri-miR-275-SV40). [score:3]
Subsequent luciferase assays showed that luciferase activity in cells co -transfected with the bmo-miR-275 recombinant expression vector and the expression vector containing the 3′UTR of BmSer-2 fused with the reporter gene was significantly decreased by nearly 29% (p < 0.05) compared to that in the control group (Fig 6). [score:3]
Expression analyses of bmo-miR-275, BmSer-2, and U6 in different tissues of 5th instar,3-day larvae of B. mori. [score:3]
bmo-miR-275 was predicted to have the potential to target BmSer-2 3’UTR. [score:3]
Semiquantitative RT-PCR for bmo-miR-275 and its predicted target genes in B. mori. [score:3]
In this study, we used bioinformatics analysis and found that BmSer-2 mRNA is a target of bmo-miR-275. [score:3]
0190464.g006 Fig 6Effect of bmo-miR-275 expressionon luciferase activity in transfected BmN cells. [score:3]
Temporal and spatial characteristics of the expression of bmo-miR-275 and its target gene in BmSer-2. Primers used in this study. [score:2]
Electrophoresis pattern of RT-PCR products ofbmo-miR-275. [score:1]
0190464.g002 Fig 2Prediction of the binding sites of bmo-miR-275 in BmSer-2 mRNA using RNAhybrid software. [score:1]
0190464.g005 Fig 5(A) pcDNA3.0 [ie1- egfp-SV40]+pGL3.0 [A3- luc- Ser-2-3’UTR-SV40]+pRL-CMV, bright light;(B) pcDNA3.0 (ie1 -egfp-SV40)+ pGL3.0 (A3-luc-Ser-2-3’UTR-SV40)+pRL-CMV, fluorescence;(C) pcDNA3.0 (ie1- egfp-pri-bmo-miR-275-SV40)+ pGL3(A3-luc- Ser-2-3’UTR-SV40)+ pRL-CMV, brightlight;(D) pcDNA3.0 (ie1- egfp-pri-bmo-miR-275-SV40)+ pGL3(A3-luc- Ser-2-3’UTR-SV40)+ pRL-CMV, fluorescence. [score:1]
Moreover, bmo-miR-275 binds BmSer-2 3’UTR starting from the 39th base and the folding energy is −16.3 kcal/mol, suggesting that bmo-miR-275 function independently (Fig 2). [score:1]
The bmo-miR-275 mature sequence of B. mori was obtained through sequencing conducted by Liu et al[20]. [score:1]
Prediction of the binding sites of bmo-miR-275 in BmSer-2 mRNA using RNAhybrid software. [score:1]
The fragment containing the enhanced green fluorescent protein-encoding gene, egfp, and the nucleotide sequences of precursors of the bmo-miRNA-275 and their up and down-stream flanking regions which were downloaded from SilkBase (http://silkbase. [score:1]
M1: DL 2000 DNA marker; M2:λ-HindШ Marker; 1: pcDNA3.0 [ie1- egfp-pri-bmo-miR-275-SV40] after double digestion with HindIII and BamHI. [score:1]
Electrophoresis showed a fragment with a length of 60–80 bp, which was consistent with the theoretical length of the bmo-miR-275 precursor fragment (Fig 1). [score:1]
Double enzyme digestion of recombinant plasmid pcDNA3.0 [ie1- egfp-pri-bmo-miR-275-SV40] with HindIII and BamHI. [score:1]
Regulation of BmSer-2 by bmo-miR-275, as determined by luciferase activity assays. [score:1]
0190464.g004 Fig 4Double enzyme digestion of recombinant plasmid pcDNA3.0 [ie1- egfp-pri-bmo-miR-275-SV40] with HindIII and BamHI. [score:1]
In the treatment group, BmN cells were co -transfected with a mixture of pcDNA3.0 [ie1- egfp-pri-bmo-miR-275-SV40], pGL3.0 [A3- luc- Ser-2-3’UTR-SV40], and pRL-CMV. [score:1]
Identification of bmo-miR-275. [score:1]
0190464.g001 Fig 1 (M: 20 bp marker;1: bmo-miR-275). [score:1]
[1 to 20 of 37 sentences]
2
[+] score: 82
Stage-by-stage examinations revealed further expression patterns, such as emergence at specific time-points during embryogenesis and up-regulation of miRNA groups in late embryos (miR-1 and bantam), expression associated with stage transition between instar and molt larval stages (miR-34b), expression associated with silk gland growth and spinning activity (miR-274), continuous high expression from the spinning larval to pupal and adult stages (miR-252 and miR-31a), a coordinate expression trough in day 3 pupae of both sexes (miR-10b and miR-281), up-regulation in pupal metamorphosis of both sexes (miR-29b), and down-regulation in pupal metamorphosis of both sexes (miR-275). [score:20]
Our results show that miRNAs display a wide variety of expression profiles over the whole life of the silkworm, including continuous expression from embryo to adult (miR-184), up-regulation over the entire life cycle (let-7 and miR-100), down-regulation over the entire life cycle (miR-124), expression associated with embryogenesis (miR-29 and miR-92), up-regulation from early 3 [rd ]instar to pupa (miR-275), and complementary pulses in expression between miR-34b and miR-275. [score:18]
Four miRNAs (miR-29b, miR-34b, miR-277, and miR-285) were significantly up-regulated from the spinning larvae to adult stages, and nine (miR-305, miR-275, miR-289, miR-307-3p, miR-274, miR-286, miR-87, miR-315, and miR-92) were significantly down-regulated during this time-course (Additional file 12). [score:7]
In contrast to miR-34b, a faint expression signal from miR-275 was initially detected at early 3 [rd ]molt, followed by slight up-regulation in early 4 [th ]instar, early 4 [th ]molt, and early 5 [th ]instar larvae. [score:6]
Moreover, both and microarray tests revealed down-regulation of miR-275 in both sexes during this process (Figure 5B, E). [score:4]
The expression profiles of miR-275 and miR-34b were regulated in a complementary manner throughout the penultimate and final larval stages (Figure 3D). [score:4]
The whole-life array revealed that, in contrast to miR-29b, miR-275 expression peaked in early female pupae, but not in the early female moth (Figures 1B, C). [score:3]
For example, miR-275 and miR-274 expression was typically stage-specific and unrelated to ecdysone pulsing. [score:3]
The changing expression profile of miR-275 by microarray was supported by results (Figure 4B). [score:3]
However, when the eggs were manually removed from the maternal abdomen, miR-275 expression was significantly increased, further confirming its absence in pre-laid eggs and early embryo stages. [score:3]
The use of alternative probes for some miRNAs generally resulted in similar expression profiles (for example, in the case of miR-275# and miR-275), strongly confirming probe authenticity. [score:3]
The expression patterns of miR-34b and miR-275 fluctuated in a complementary manner (Figure 1B). [score:3]
Unexpectedly, miR-275 was not expressed in the early female moth with eggs. [score:3]
miR-34b and miR-275 exhibited complementary changes in patterns in whole-life profiling, but displayed sympathetic vibrations, particularly from the early 2 [nd ]instar to late 3 [rd ]molt stages, upon more detailed examination (Figure 3B). [score:1]
Alternative probes of the specific miRNAs clearly yielded identical results (let-7a and let-7a#; miR-275 and miR-275#). [score:1]
[1 to 20 of 15 sentences]
3
[+] score: 36
Expression pattern of Bmo-miR-275 was significantly increased in the body wall, silk glands, midgut and fat body during metamorphosis [18] and it was up-regulated from early 3rd instar to pupa but down-regulated in pupal metamorphosis of male and female [41]. [score:9]
Analysis of putative target genes exhibits that the potential target genes to 6 differentially expressed miRNA, including bmo-miR-275, bmo-miR-14, bmo-miR-1a, N-50, N-46 and N-45, were classified into response to stimulus and immune system process based on GO analysis. [score:7]
Using qRT-PCR approach, 6 up-regulated miRNAs (bmo-miR-275, N-11, N-45, N-58, N-46 and N-50) and 4 down-regulated miRNAs (bmo-miR-14, bmo-miR-1a, bmo-miR-9a and bmo-miR-274) were validated for the sequence data. [score:7]
In addition, miR-275 down-regulates a key differentiation factor, Bag of marbles (Bam) to ensure proper terminal differentiation in the Drosophila male germline [42]. [score:4]
Bryant et al [43] reported that miR-275 in A. aegypti females is regulated by a steroid hormone, 20-hydroxyecdysone and indispensable for some physiological processes including blood digestion, fluid excretion and egg development. [score:3]
Similarly, Bmo-miR-275 targets several genes including BGIBMGA001084-TA, BGIBMGA004198-TA, BGIBMGA009211-TA, BGIBMGA003614-TA, and BGIBMGA005392-TA. [score:3]
Recent studies showed that miR-275 plays important role in development and metamorphosis process of Bombyx mori [18], [41]. [score:2]
The transcript level of bmo-miR-275 in the infected midgut at 96 h post inoculation was higher than that in the control midgut. [score:1]
[1 to 20 of 8 sentences]
4
[+] score: 22
Expression patterns of five miRNAs were significantly increased during metamorphosis in all four tissues (e. g., bmo-miR-275 and bmo-miR-305), and two miRNA pairs, bmo-miR-10b-3p/5p and bmo-miR-281-3p/5p, showed coordinate expression. [score:5]
Among these miRNAs, miR-275 and miR-305 are clustered in the silkworm genome [30] and were found to be coordinately upregulated. [score:4]
In contrast to miR-34b, low levels of miR-275 and miR-305 were confirmed with both methods in the four tissues of fifth-instar day 7 larvae, but strong expression was verified in the body wall, midgut and fat body from the spinning larval to pupal stages. [score:3]
Specifically, 13 were significantly expressed in the ovaries (e. g., miR-34b, miR-275, miR-305, and let-7a) and four in the testes (miR-252, miR-29b, miR-7, and miR-228,). [score:3]
In all, only 10 miRNAs were universally distributed (including bmo-let-7 and bmo-bantam), while the majority were expressed exclusively or preferentially in specific tissue types (e. g., bmo-miR-275 and bmo-miR-1). [score:3]
Sex -dependent expression of miR-275 was also observed in the gonads and hemocyte on microarray blocks with two different probes. [score:3]
Both microarray and analyses with alternative probes for bmo-miR281-5p, bmo-miR-10b-5p, bmo-miR-1, bmo-let-7a and bmo-miR-275 yielded reproducible results. [score:1]
[1 to 20 of 7 sentences]
5
[+] score: 12
For instance, aside from a few commonly expressed miRNAs, such as bmo-miR-8, bmo-miR-13, bmo-miR-263a, bmo-miR-275, bmo-miR-279, bmo-282*, bmo-miR-285 and bmo-miR-306, most of them were found development-related (Table 3) although some may be due to inadequate sampling that often results less reliable statistics. [score:4]
The expression of bmo-miR-7 and bmo-miR-275 maintained at a relatively stable level in 14 development stages except bmo-miR-275 alone displayed a weak increase at both PPS and PS. [score:4]
For analyzing the expression patterns of bmo-miR-1, bmo-miR-13, bmo-miR-14, bmo-miR-77, bmo-miR-263a, bmo-miR-275 and U6 snRNA, real-time PCR was performed in an ABI Prism® 7300 Sequence Detection system with Quant SYBR Green PCR kit (TIANGEN, BJ) following the manufacturer's instructions. [score:3]
We also discovered six pairs that are organized as clusters; bmo-miR-1/bmo-miR-133, bmo-let-7/bmo-miR-100, bmo-miR-12/bmo-miR-283, and bmo-miR-275/bmo-miR-305 are separated by less than 20 kb apart and in the same orientation; bmo-miR-9b overlaps with bmo-miR-79 on the opposite strand; and bmo-miR-2 is adjacent to bmo-miR-13 but on the reverse strand in a tail-to-tail orientation about some twenty basepairs away. [score:1]
[1 to 20 of 4 sentences]
6
[+] score: 7
Liu et al. (2010a) were the first to report spatial expression patterns of nearly 100 miRNAs in multiple normal tissues of female and male B. mori using microarray and northern-blotting analyses, in which only 10 miRNAs were detected to express in universal tissue types of the B. mori, such as bmo-let-7 and bmo-bantam, whereas the majority were distributed exclusively or preferentially in specific tissues, such as bmo-miR-275 and bmo-miR-1. Other experimental protocols commonly used to detect miRNA expressions are polymerase chain reaction (PCR) technology, polyacryla-mide gel electrophoresis (PAGE)/northern blotting, and real-time PCR (qPCR). [score:7]
[1 to 20 of 1 sentences]
7
[+] score: 5
Examining the positions of the identified miRNAs in the B. mori genome, we identified two miRNA clusters (Figure 4): bmo-miR-2a-1/bmo-miR-2a-1*/bmo-miR-2a-2/bmo-miR-2b/bmo-miR-13a*/bmo-miR-13b; and bmo-miR-275/bmo-miR-305/bmo-miR-305*. [score:1]
a. bmo-miR-2a-1/bmo-miR-2a-1*/bmo-miR-2a-2/bmo-miR-2b/bmo-miR-13a*/bmo-miR-13b; b. bmo-miR-275/bmo-miR-305/bmo-miR-305*. [score:1]
The cluster bmo-miR-275/bmo-miR-305/bmo-miR-305* is also found in D. melanogaster, D. pseudoobscura and A. gambiae. [score:1]
Members of the bantam, mir-71, mir-275, mir-277, mir-279 miRNA families have a high degree of similarity in their precursor sequences. [score:1]
bmo-miR-275 and bmo-miR-305 belong to different miRNA families, mir-275 and mir-305. [score:1]
[1 to 20 of 5 sentences]
8
[+] score: 3
The contrary results were found among bmo-let-7, bmo-miR-1, bmo-miR-100, bmo-miR-124, bmo-miR-137, bmo-miR-14, bmo-miR-252, bmo-miR-275, bmo-miR-305, bmo-miR-307, bmo-miR-34, and bmo-miR-279c, where the literature -based collections showed higher expression levels (Additional file 12: Table S7). [score:3]
[1 to 20 of 1 sentences]
9
[+] score: 1
It includes miR-1, the entire family of miR-2, the miR-9 family (miR-9 and miR-9b), the let-7 family (let-7a, let-7j), miR-10b, miR-31, miR-71, miR-79, miR-87, miR-98, miR-100, miR-252, miR-263a, miR-275, miR-279, miR-317 and miR-1274b (Table 3 and Figure 4a). [score:1]
[1 to 20 of 1 sentences]
10
[+] score: 1
For example, the read counts of bmo-miR-275 are almost 10-fold greater than those of bmo-miR-305 in the anterior-middle and posterior silk gland, but only two-fold greater relative to the whole body. [score:1]
[1 to 20 of 1 sentences]