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8 publications mentioning mmu-mir-450a-2

Open access articles that are associated with the species Mus musculus and mention the gene name mir-450a-2. Click the [+] symbols to view sentences that include the gene name, or the word cloud on the right for a summary.

1
[+] score: 183
In summary, we find that overexpression of miR-450a-3p can induce Bub1 down-regulation, leading to the inhibition of cell proliferation, an increase in apoptosis and restriction of most cells in the G1 phase. [score:8]
Under these conditions, the expression of Bub1 mRNA did not change (Fig. 2C ), suggesting that miR-450a may target Bub1 mRNA through its suppression rather than its degradation. [score:7]
Our results have demonstrated that overexpression of miR-450a-3p represses iMEF cell proliferation and down-regulates Bub1 protein level. [score:6]
To examine whether miR-450a-3p down-regulates the mRNA expression of Bub1 in iMEFs, miR-450a-3p -mimic was transfected into iMEFs, followed by qRT–PCR analysis of Bub1 mRNA. [score:6]
The miR-450a-3p down-regulates Bub1 expression at the transcriptional level. [score:6]
Our result shows that miR-450a-3p targets mice Bub1 mRNA 3′-UTR and down-regulate Bub1 protein. [score:6]
However, the percentage of the Hoechst positive cells with miR-450a-3p was low, which may at least partially explain the marked inhibition of proliferation caused by miR-450a-3p expression may be not entirely caused by apoptosis. [score:5]
We found that treatment of iMEF cells with miR-450a-3p mimic (50 nM) positively caused a marked reduction of Bub1 expression, whereas treatment with miR-30a or 30e mimic or an negative control (non -targeting) mimic did not cause any reduction of Bub1 protein (Fig. 2A ). [score:5]
Overexpression of Bub1 reduces the proliferation inhibition caused by miR-450a-3p. [score:5]
The action of miR-450a-3p is at least in part through down-regulation of Bub1. [score:4]
Microinjection of miR-450a-3p suppresses embryo development. [score:4]
Among them, miR-450a-3p is confirmed to directly target Bub1. [score:4]
This suggests that miR-450a-3p is likely to be a regulator of Bub1 through targeting the Bub1 3′-UTR. [score:4]
However, the extent of inhibition of Bub1 by miR-450a-3p is much less than that through gene knockout technology. [score:4]
Bub1 is required for miR-450a-directed iMEFs proliferation inhibition. [score:4]
The mechanism by which miR-450a-3p negatively regulates Bub1 is translational repression through imperfect base pairing with the 3′-UTR. [score:4]
These findings suggest that the repression of cell proliferation by miR-450a may be caused by the down-regulation of Bub1. [score:4]
In this study, we focused on a well-known spindle assembly checkpoint protein Bub1 and validated it as a direct target of miR-450a-3p. [score:4]
Thus, down-regulation of Bub1 by the introduction of miR-450a-3p -mimic was successfully rescued by Ad-Bub1 infection. [score:4]
Overexpression of miR-450a-3p increased the fraction of cells in the G1 phase from 43.34% to 62.09% while decreasing the fraction of cells in the G2/M phase from 19.08% to 11.82% (Fig. 3D ). [score:3]
For examining the expression of Bub1 in embryos, zygotes microinjected with miR-450a-3p or control mimic were collected, rinsed twice in PBS, resuspended in 10 ml of Laemmli buffer and boiled for 10 minutes. [score:3]
Therefore, it is not surprising that microinjection of miR-450a-3p can suppress zygote cleavage. [score:3]
The expression of Bub1 in miR-450a-3p treated zygotes was also confirmed (Fig. 5C ). [score:3]
When the fertilized eggs are microinjected with miR-450a-3p mimics, the cleavage of zygotes is effectively suppressed. [score:3]
We further reveal that miR-450a-3p suppresses cell proliferation and impairs cell cycle progression. [score:3]
These results indicate that the 3′-UTR of Bub1 is likely a target of miR-450a-3p (Fig. 1C ). [score:3]
In an effort to confirm that the Bub1 3′-UTR is a target for miR-450a-3p, we transfected into HEK 293 cells a luciferase reporter containing a perfect complementary MRE (miRNA response element, MRE) of miR-450a-3p to provide a site with high binding affinity for the miRNA. [score:3]
miR-450a-3p suppresses iMEF cell proliferation. [score:3]
We next tested if ectopic expression of miR-450a-3p would reduce Bub1 protein level in iMEF cells. [score:3]
The suppression of luciferase activity was induced only by Ad-miR-450a-3p. [score:3]
We also find that overexpression of miR-450a-3p can increase apoptosis in iMEF cells, which is supported by other reports. [score:3]
The 3′-UTR of Bub1 is targeted by miR-450a-3p. [score:3]
There was a significant decrease in Bub1 protein expression in embryos microinjected with miR-450a-3p relative to the mimic control or the untreated control. [score:3]
Effect of miR-450a-3p overexpression on proliferation and apoptosis in iMEFs cells. [score:3]
Over -expression of miR-450a-3p significantly reduced cell divisions of zygotes when compared to those injected with scramble control. [score:2]
The mutations prevented miR-450a-3p from interfering with luciferase activity. [score:2]
0047914.g005 Figure 5(A) There are three cases of zygotic development when 2-cell embryos received microinjections of miR-450a-3p or control mimic: ‘a’ denotes zygotes that developed to the 8-cell embryo; ‘b’ denotes zygotes that developed to the 4-cell embryo but failed to reach the 8-cell stage; ‘c’ denotes zygotes that failed to reach the 4-cell embryo stage. [score:2]
Taken together, these results strongly suggest that miR-450a-3p may play an important role in regulating Bub1 level and a abnormally low level of Bub1 may lead to spontaneous miscarriage. [score:2]
We also demonstrate that miR-450a-3p is involved in embryo development. [score:2]
At 48 h the luciferase activity was significantly suppressed by 54% (p<0.001) in HEK 293 cells treated with pMIR-3′ vector and miR-450a-3p -mimic, compared with control (Fig. 1A ). [score:2]
miR-450a-3p leads to down regulation of Bub1 protein. [score:2]
Nonetheless, these findings indicate that Bub1 can be negatively regulated by miR-450a-3p in iMEF cells. [score:2]
Flow cytometry analysis was performed to examine whether miR-450a-3p alters the cell cycle progression. [score:1]
Computational analysis predicted that the 3′-UTR of Bub1 transcript contains a 14-mer miR-450a-3p nucleotides complementation after the stop codon (Fig. 1B ). [score:1]
About 10 pL of 25 mM miR-450a-3p -mimic was microinjected into the zygotes' cytoplasm. [score:1]
We tested five doses of chemically synthetic miR-450a-3p -mimic in iMEFs, followed by of Bub1 protein. [score:1]
To validate this result, we mutated the miR-450a-3p binding site on Bub1 3′-UTR (Fig. 1B, Bub1 mut1 and mut2). [score:1]
Cell proliferation was measured by the Cell Counting Kit-8. We found that over -expression of miR-450a-3p repressed cell proliferation and there was a nearly 40% reduction of proliferation after transfection at 72 h (Fig. 3A ). [score:1]
Anti-proliferative effect observed in iMEFs treated with miR-450a-3p is less obvious than which in Bub1 -null (repressing Bub1 by ∼98%) primary embryonic fibroblasts. [score:1]
Various concentrations of miR-450a-3p mimic (3 nM, 10 nM, 50 nM, 100 nM, 200 nM) were transfected into iMEFs (Fig. 2B ). [score:1]
Effects of miR-450a-3p on zygote cleavage. [score:1]
Interestingly, the levels of Bub1 protein did not significantly decrease with the higher concentration of miR-450-3p mimic (Fig. 2B ). [score:1]
Statistically significant differences (* p<0.05) were found between the miR-450a-3p -injected embryos and the control -injected embryos. [score:1]
After transfected with miR-450a-3p mimic or control mimic for 48 hours, iMEF cells were harvested and stained with Propidium Iodide (PI). [score:1]
After 48 h, the numbers of Hoechst -positive cells treated with miR-450a-3p were more than that treated with control mimic (Fig. 3B ). [score:1]
Microinjection of miR-450a-3p -mimic into the two-cell embryos was carried out. [score:1]
Bub1 rescue in miRNA-450a-3p treated cells led to an increase in cell numbers in S phase and a decrease of cell apoptosis (Fig. 4D and 4E ). [score:1]
The proliferation rate was higher in cells co-transduced with miRNA-450a-3p and Ad-Bub1 (Fig. 4C ). [score:1]
However, higher concentrations (e. g., 50 nM) of miR-450a-3p -mimic significantly reduced Bub1 protein to a lower level than that in iMEFs with the negative control (Fig. 2B ). [score:1]
We next examined the functional relevance of the miR-450a-3p/Bub1 interaction in mouse embryo fibroblasts. [score:1]
These results suggest that miR-450a-3p may repress cell proliferation by arresting cells in the G1 phase. [score:1]
The miR-450a-3p complementary luciferase vector was constructed by inserting an oligo containing two consecutive perfect matches to miR-450a-3p to the luciferase gene in the pMIR-Report. [score:1]
The iMEF cells were infected with Bub1 adenoviral vector (Ad-Bub1) or a control vector (Ad-RFP), and then transfected with or without miRNA-450a-3p mimic. [score:1]
The percent of Hoechst positive cells treated with miR-450a-3p is 6.8%, while the percent of Hoechst positive cells treated with miR-30a, miR-30e and the control is 2.4%, 1.5%, and 2.1% respectively (Fig. 3C ). [score:1]
miR-450a-3p binds to the 3′ UTR of mouse Bub1 mRNAs. [score:1]
The results of flow cytometry analysis confirmed that cells treated with miR-450a-3p were mostly arrested at the G1 phase. [score:1]
Cell lysates were prepared in lysis buffer following 48 h transfection with either different concentration miR-450a-3p -mimic or control mimic. [score:1]
Bub1 protein level was higher in cells transduced with miRNA-450a-3p -mimic and Ad-Bub1 than that in the cells transduced with miRNA-450a-3p -mimic and Ad-RFP (Fig. 4B ). [score:1]
About 28.26% of the miR-450a-3p -injected zygotes failed to develop into the 8-cell stage and 34.47% failed to develop into the 4-cell, while 28.15% of the scramble control -injected zygotes failed to develop into the 8-cell stage and 14.42% failed to develop into the 4-cell stage (Fig. 5A and 5B ). [score:1]
Thus, the blockade of miR-450a-3p may constitute a novel therapeutic strategy for preventing spontaneous miscarriages. [score:1]
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2
[+] score: 10
Other miRNAs from this paper: hsa-let-7c, hsa-let-7d, hsa-mir-16-1, hsa-mir-21, hsa-mir-24-1, hsa-mir-24-2, hsa-mir-28, hsa-mir-29a, hsa-mir-30a, hsa-mir-31, hsa-mir-99a, hsa-mir-101-1, hsa-mir-16-2, mmu-let-7g, mmu-let-7i, mmu-mir-27b, mmu-mir-30a, mmu-mir-99a, mmu-mir-101a, mmu-mir-125b-2, mmu-mir-126a, mmu-mir-128-1, mmu-mir-9-2, mmu-mir-142a, mmu-mir-144, mmu-mir-145a, mmu-mir-151, mmu-mir-152, mmu-mir-185, mmu-mir-186, mmu-mir-24-1, mmu-mir-203, mmu-mir-205, hsa-mir-148a, hsa-mir-34a, hsa-mir-203a, hsa-mir-205, hsa-mir-210, hsa-mir-221, mmu-mir-301a, mmu-let-7d, hsa-let-7g, hsa-let-7i, hsa-mir-27b, hsa-mir-125b-1, hsa-mir-128-1, hsa-mir-142, hsa-mir-144, hsa-mir-145, hsa-mir-152, hsa-mir-9-1, hsa-mir-9-2, hsa-mir-9-3, hsa-mir-125b-2, hsa-mir-126, hsa-mir-185, hsa-mir-186, mmu-mir-148a, mmu-mir-200a, mmu-let-7c-1, mmu-let-7c-2, mmu-mir-16-1, mmu-mir-16-2, mmu-mir-21a, mmu-mir-24-2, mmu-mir-29a, mmu-mir-31, mmu-mir-34a, mmu-mir-148b, mmu-mir-339, mmu-mir-101b, mmu-mir-28a, mmu-mir-210, mmu-mir-221, mmu-mir-9-1, mmu-mir-9-3, mmu-mir-125b-1, mmu-mir-128-2, hsa-mir-128-2, hsa-mir-200a, hsa-mir-101-2, hsa-mir-301a, hsa-mir-151a, hsa-mir-148b, hsa-mir-339, hsa-mir-335, mmu-mir-335, hsa-mir-449a, mmu-mir-449a, hsa-mir-450a-1, mmu-mir-450a-1, hsa-mir-486-1, hsa-mir-146b, hsa-mir-450a-2, hsa-mir-503, mmu-mir-486a, mmu-mir-542, mmu-mir-503, hsa-mir-542, hsa-mir-151b, mmu-mir-301b, mmu-mir-146b, mmu-mir-708, hsa-mir-708, hsa-mir-301b, hsa-mir-1246, hsa-mir-1277, hsa-mir-1307, hsa-mir-2115, mmu-mir-486b, mmu-mir-28c, mmu-mir-101c, mmu-mir-28b, hsa-mir-203b, hsa-mir-5680, hsa-mir-5681a, mmu-mir-145b, mmu-mir-21b, mmu-mir-21c, hsa-mir-486-2, mmu-mir-126b, mmu-mir-142b, mmu-mir-9b-2, mmu-mir-9b-1, mmu-mir-9b-3
Furthermore, some of the differentially expressed miRNAs have been reported to play a role in the metastasis of other types of cancer, for example, the up-regulated miRNAs, let-7i, miR-9, miR-30a, miR-125b, miR-142-5p, miR-151-3p, miR-450a and the down-regulated miRNAs, miR-24, mir-145, miR-146b-5p, miR-185, miR-186, miR-203 and miR-335. [score:9]
The miR-30a, miR-142-5p and miR-450a have roles in metastatic breast and colon cancer [61] and the miR-151-3p can enhance hepatocellular carcinoma cell mobility [66]. [score:1]
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3
[+] score: 9
Other miRNAs from this paper: hsa-let-7a-1, hsa-let-7a-2, hsa-let-7a-3, hsa-let-7b, hsa-let-7c, hsa-let-7d, hsa-let-7e, hsa-let-7f-1, hsa-let-7f-2, hsa-mir-96, mmu-let-7g, mmu-let-7i, mmu-mir-124-3, mmu-mir-9-2, mmu-mir-141, mmu-mir-152, mmu-mir-182, mmu-mir-183, mmu-mir-199a-1, hsa-mir-199a-1, mmu-mir-200b, mmu-mir-205, hsa-mir-7-1, hsa-mir-7-2, hsa-mir-7-3, hsa-mir-182, hsa-mir-183, hsa-mir-199a-2, hsa-mir-199b, hsa-mir-205, hsa-mir-214, hsa-mir-200b, mmu-let-7d, mmu-mir-130b, hsa-let-7g, hsa-let-7i, hsa-mir-124-1, hsa-mir-124-2, hsa-mir-124-3, hsa-mir-141, hsa-mir-152, hsa-mir-9-1, hsa-mir-9-2, hsa-mir-9-3, mmu-mir-200a, mmu-let-7a-1, mmu-let-7a-2, mmu-let-7b, mmu-let-7c-1, mmu-let-7c-2, mmu-let-7e, mmu-let-7f-1, mmu-let-7f-2, mmu-mir-96, hsa-mir-200c, mmu-mir-200c, mmu-mir-214, mmu-mir-199a-2, mmu-mir-199b, mmu-mir-124-1, mmu-mir-124-2, mmu-mir-9-1, mmu-mir-9-3, mmu-mir-7a-1, mmu-mir-7a-2, mmu-mir-7b, hsa-mir-200a, hsa-mir-130b, hsa-mir-376a-1, mmu-mir-376a, dre-mir-7b, dre-mir-7a-1, dre-mir-7a-2, dre-mir-182, dre-mir-183, dre-mir-199-1, dre-mir-199-2, dre-mir-199-3, dre-mir-205, dre-mir-214, hsa-mir-429, mmu-mir-429, hsa-mir-450a-1, mmu-mir-450a-1, dre-mir-429a, dre-let-7a-1, dre-let-7a-2, dre-let-7a-3, dre-let-7a-4, dre-let-7a-5, dre-let-7a-6, dre-let-7b, dre-let-7c-1, dre-let-7c-2, dre-let-7d-1, dre-let-7d-2, dre-let-7e, dre-let-7f, dre-let-7g-1, dre-let-7g-2, dre-let-7h, dre-let-7i, dre-mir-7a-3, dre-mir-9-1, dre-mir-9-2, dre-mir-9-4, dre-mir-9-3, dre-mir-9-5, dre-mir-9-6, dre-mir-9-7, dre-mir-96, dre-mir-124-1, dre-mir-124-2, dre-mir-124-3, dre-mir-124-4, dre-mir-124-5, dre-mir-124-6, dre-mir-130b, dre-mir-141, dre-mir-152, dre-mir-200a, dre-mir-200b, dre-mir-200c, hsa-mir-450a-2, dre-let-7j, hsa-mir-376a-2, dre-mir-429b, mmu-let-7j, mmu-let-7k, mmu-mir-9b-2, mmu-mir-124b, mmu-mir-9b-1, mmu-mir-9b-3
We found eight miRs differentially expressed, six down-regulated (miR-9, miR-141, miR-200a, miR-200b, miR-429 and miR-376a) and two up-regulated (miR-450a-5p and miR130b*) in the Dlx5 [−/−] OE (Fig.  1a). [score:9]
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4
[+] score: 9
This may be due to transcriptional regulation [98] but possibly also to the repression of miRNAs (mmu-miR-1 at ST; mmu-miR-450a-5p at IT; mmu-miR-27a and -92a at LT) that target IGF1 mRNA. [score:4]
The selection of the miRNAs is based on their potential role in the pathology of the lung (mmu-miR-1, 146b, -203, -21, -223, -29b, -29c) or on their high and significant differential expression in the mo del (mmu-miR-455, -574-5p, -672, -690) or for their high (mmu-let-7b a, mmu-miR-145) or low (mmu-miR-450a-5p) signal intensity in microarray analysis. [score:3]
Among these data, 40 out of 42 (95%) gave results and trends similar to those obtained by microarray profiling, except for mmu-miR-29c at ST and LT (Figure 2), although the magnitude of the observed regulation was not always identical (mmu-miR-455 at LT and mmu-miR-450a at IT). [score:2]
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5
[+] score: 6
While very little is known about miR-450a to support its selection as a candidate for further investigation on its potential role in MGO -treated MAECs, it has been shown that miR-126 is able to downregulate IRS-1, suppressing Akt activation [36, 37]. [score:4]
We confirmed that 4 out of these 84 miRNAs were significantly altered in MGO -treated MAECs: miR-126, miR-190a, miR-214 and miR-450a [21]. [score:1]
By our previous investigations, we have also found that the expression of other three miRNAs is related to diabetes: miR-214, miR-450a, and miR-126 are reduced by 32%, 22%, and 30%, respectively, in MAECs exposed to MGO [21]. [score:1]
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6
[+] score: 3
NI MV OpenArray RT-qPCR hsa-miR-328 − 2.5* ± 0.93Not tested [a] hsa-miR-335* − 3.0* ± 1.13Not tested [a] mmu-miR-16* 2.8** ± 0.65Not tested [a] mmu-miR-21* 5.0** ± 0.88Not tested [a] mmu-miR-297a* 5.8* ± 1.60Not tested [a] mmu-miR-685 3.0* ± 1.00Not tested [a] mmu-miR-1949 5.0* ± 1.69Not tested [a] hsa-miR-590-5p Unique to NINot validated [b] rno-miR-450 Unique to CMNot validated [b] mmu-miR-10b 2.7* ± 0.85Not validated [b] hsa-miR-146a 3.2** ± 0.68 7.2* ± 2.74 hsa-miR-150 1.8* ± 0.64 2.7 (ns) ± 2.26 hsa-miR-205 2.3* ± 0.75 − 0.5 (ns) ± 1.89 hsa-miR-486 2.3*** ± 0.18 4.7 (ns) ± 1. 45 mmu-miR-193b − 2.7** ± 0.62 − 7.5* ± 0 62 mmu-miR-215 2.1* ± 0.554.6 (ns) ± 99.39 [c] mmu-miR-467a − 2.0* ± 0.69 − 5.6 (ns) ± 0.96 The list of significantly differentially expressed miRNA in CM vs NI MV from the was compared with the results obtained by. [score:2]
Two additional miRNA—miR-590-5p and miR-450—were selected among those unique to either CM or NI MV for verification; however, as amplification was not adequate across all samples and biological groups, data for these two miRNA as well as for miR-10b, were not shown (Table  1). [score:1]
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7
[+] score: 1
Taken together, these elements may represent a multipart transcript, and interestingly four additional miRNA coding sites (mir-542, mir-450a-2, mir-450a-1, and mir-450b) reside nearby, which do not have any evidence of being transcribed based on known dbEST sequence records. [score:1]
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8
[+] score: 1
miRNA name (Release 17)† Accession Number miRNA name (Release 20)† Fold Changes P-Value (8)‡ P-Value (5)ǁ FDR (8)‡ mmu-miR-696 MIMAT0003483 mmu-miR-696 0.54 2.66E-03 3.65E-02 6.33E-02 mmu-miR-449c MIMAT0003460 mmu-miR-449c-5p 0.58 2.70E-06 9.81E-04 8.86E-04 mmu-miR-7a MIMAT0000677 mmu-miR-7a-5p 0.60 1.67E-03 3.66E-03 5.12E-02 mmu-miR-205 MIMAT0000238 mmu-miR-205-5p 0.62 3.03E-01 3.86E-03 7.56E-01 mmu-miR-450a-2* MIMAT0004789 mmu-miR-450a-2-3p 0.63 1.00E-04 7.93E-03 9.42E-03 mmu-miR-1199* MIMAT0017333 mmu-miR-1199-3p 0.63 1.40E-04 1.10E-02 1.02E-02 mmu-miR-374c MIMAT0014953 mmu-miR-374c-5p 0.63 8.79E-03 1.62E-02 1.56E-01 mmu-miR-218 MIMAT0000663 mmu-miR-218-5p 0.64 1.72E-03 3.82E-03 5. 12E-02 mmu-let-7b* MIMAT0004621 mmu-let-7b-3p 0.66 4.01E-04 4.74E-03 2.19E-02† Based on http://www. [score:1]
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