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5 publications mentioning dre-mir-212

Open access articles that are associated with the species Danio rerio and mention the gene name mir-212. Click the [+] symbols to view sentences that include the gene name, or the word cloud on the right for a summary.

1
[+] score: 165
Furthermore, the expression pattern of miRNA-212 during early embryogenesis is inversely correlated with FIGLA expression during early embryogenesis, such that miR-212 expression increases steadily from 2-cell to 8-cell stage of embryogenesis, while FIGLA expression decreases gradually during the same period. [score:9]
In human lung cancer [54], pancreatic cancer [55], breast cancer [56] and colorectal cancer [57], miR-212 expression was significantly up regulated, whereas in osteosarcoma [58] and gastric cancers [59], the expression of miR-212 has been shown to be down-regulated. [score:9]
Since we have shown that miR-212 is capable of regulating FIGLA expression through direct binding to the 3’ UTR of its mRNA and that miR-212 is differentially expressed during early embryogenesis, we next investigated whether miR-212 regulates FIGLA expression in early embryos. [score:8]
Western blot analysis with an antibody against FLAG shows a significant inhibition of FLAG-tagged FIGLA protein expression in cells expressing miR-212 compared to the control cells without miR-212 (Figure 5A), indicating that translation of FIGLA is repressed by miR-212. [score:8]
Third, miR-212 suppresses the activity of a luciferase reporter fused with the 3`UTR of FIGLA in a MRE dependent manner, and finally, ectopic expression of miR-212 mimic in bovine early embryos significantly represses FIGLA protein expression. [score:7]
Expression analysis during oocyte maturation and early embryonic development showed that miR-212 is expressed in GV oocytes and tends to increase at the 4-cell and 8-cell stage embryos followed by a decline at morula and blastocyst stages (Figure 4B). [score:6]
First, the expression of miR-212 is inversely correlated to the expression of FIGLA during bovine early embryonic development. [score:6]
In order to test if miR-212 is indeed capable of regulating FIGLA protein expression, we used the human cervical cancer cell line HeLa because it expresses neither FIGLA nor miR-212 (data not shown). [score:6]
Suppression of luciferase activity was abolished when the mutation was introduced into the seed region of the miRNA-212 recognition sequence in the FIGLA 3’ UTR (Figure 5B), indicating that the predicted MRE is critical for the direct and specific binding of miR-212 to FIGLA mRNA. [score:5]
Ectopic expression of miR-212 suppressed the activity of the luciferase construct containing the miR-212 binding site of FIGLA mRNA at its 3’ end. [score:5]
Deregulation of miR-212/132 has also been associated with several neurological disorders, such as Alzheimer’s disease and tauopathies [51]. [score:4]
Following LH/hCG stimulation in the ovarian cells miR-132 and miR-212 were found to be highly up regulated and computational analysis has identified nearly 77 putative mRNA as potential targets of miR-212 and miR-132 in granulosa cells [60]. [score:4]
MiR-212 was predominantly expressed in hypothalamus and brain (Figure 4A), which is consistent with the expression profile observed in mice [43]. [score:4]
The inverse correlation between miR-212 and FIGLA expression supports that miR-212 might be a post-transcriptional regulator of FIGLA during MET. [score:4]
Mutation of the miR-212 miRNA recognition element (MRE) in the FIGLA 3’ UTR was performed using the QuikChange site-directed mutagenesis kit (Stratagene) according to the manufacturer’s instructions. [score:3]
The expression of miR-212 has been reported in different kinds of tumors. [score:3]
miRNA-212 binding site prediction and expression analysis. [score:3]
Expression analysis of miR-212. [score:3]
To determine the expression profiles of miRNA-212 in bovine tissues and embryos, quantitative real-time PCR was performed. [score:3]
Effect and specificity of miRNA-212 action on FIGLA expression. [score:3]
Furthermore, secondary structure analysis revealed that the apparent miR-212 target site was positioned on a hairpin-loop structure in an exposed position, which might facilitate miRNA accessibility. [score:3]
miR-212 represses endogenous FIGLA expression in early embryos. [score:3]
Second, miR-212 represses the expression of bovine FIGLA protein in HeLa cells. [score:3]
miR-212 expression plasmid (pcDNA3.1: miR-212) was constructed by cloning a 220 bp fragment (surrounding pre-miR-212) amplified from bovine genomic DNA. [score:3]
In addition, expression of miR-212 in fetal and adult ovary was also detected (Figure 4A). [score:3]
Here, we report the cloning of the bovine orthologue of FIGLA gene, the characterization of its mRNA and protein expression during bovine oocyte maturation and early embryonic development, and the demonstration of miRNA-212 as a potential negative regulator of bovine FIGLA during bovine early embryogenesis. [score:3]
Although such direct evidence from mammals is limited, we hypothesize a similar mechanism is likely to be involved in the negative regulation of FIGLA by miR-212 in bovine embryos during MET. [score:3]
Our report is the first to identify a miRNA that directly regulates FIGLA and display a novel potential role for miR-212 during early embryogenesis. [score:3]
Effect of miR-212 on FIGLA expression in early embryos. [score:3]
Taken together, these data indicate that miR-212 binds to the 3’ UTR of FIGLA mRNA and impairs FIGLA mRNA translation. [score:3]
Besides their multiple roles in neuronal development, increasing evidence point towards an important involvement of miR-212 and miR-132 in mediating many other biological processes, including inflammation [52], immune function [53] and other cellular dysfunctions such as cancer. [score:2]
These findings suggest that miR-212 is likely an important regulator of FIGLA. [score:2]
As shown in Figure 6, microinjection of miR-212 mimic into bovine embryos effectively reduced FIGLA protein expression in 8-cell embryos compared to the uninjected and the negative control miRNA mimic injected embryos. [score:2]
The expression of miRNA-212 in multiple tissues, oocytes and early embryos was performed as described previously [32] (See Table S1 for primers used in the analysis). [score:2]
Our results support and unveil a novel potential role for miR-212 in regulating maternal mRNAs during early embryogenesis. [score:2]
We also provide several lines of evidence to support a new role for miR-212 as a bona fide negative regulator of FIGLA during early embryogenesis. [score:2]
miR-212 and miR-132 are evolutionary conserved tandem miRNAs, well known for their essential role in the development, maturation and function of neurons [42]. [score:2]
Furthermore, recent evidence has demonstrated that miR-212 and miR-132 play an important role as post-transcriptional regulators in granulosa cells [60]. [score:2]
A four-base pair mismatch mutation was introduced in the predicted MRE in the 3’ UTR of the FIGLA for miR-212 such that interaction between miR-212 and FIGLA mRNA was compromised. [score:2]
miR-212 specifically regulates bovine FIGLA in vitro in HeLa cells. [score:2]
miR-212 arises from the miR-212/132 cluster (which comprises miR-212 and miR-132). [score:1]
Furthermore, in order to validate the specificity and the efficacy of miR-212 action through the predicted binding site; we inserted FIGLA 3’ UTR sequence downstream of the firefly luciferase-coding region. [score:1]
0076114.g004 Figure 4. (A) Tissue distribution of miR-212 analyzed by quantitative real time PCR. [score:1]
Prediction of a miR-212 binding site in the 3`UTR of bovine FIGLA mRNA. [score:1]
The identified MRE has a low predicted free energy of hybridization with miR-212 (−20.86 kcal/mol), suggesting a stable miR:MRE duplex within the 6 nt seed region at the 5` end of the miRNA (Figure 3). [score:1]
miR-212 and miR-132 are closely related as they have identical seed sequences and the mature miRNA differs only by four nucleotides. [score:1]
HeLa cells were grown in culture and transiently transfected with pcDNA3.1: FLAG-FIGLA and either pcDNA3.1: miR-212 or empty pcDNA3.1 vector as a negative control. [score:1]
0076114.g006 Figure 6 Effect of miR-212 mimic microinjection on abundance of FIGLA protein in 8-cell embryos as determined by immuofluorescent staining with anti-FIGLA antibody (n = 2 pools of 20 embryos per treatment). [score:1]
0076114.g003 Figure 3Prediction of a miR-212 binding site in the 3`UTR of bovine FIGLA mRNA. [score:1]
The computational search identified a miRNA recognition element (MRE) for miR-212 in bovine FIGLA 3`UTR. [score:1]
Mature miRNA-212 mimic (MIMAT0022695) and a negative control miRNA mimic (cel-miR-67, CN- 001000-01-05) were obtained from Dharmacon Technologies (Dharmacon Inc, Lafayette, CO), and diluted with RNase free water to a final concentration of 20 µM before microinjection. [score:1]
Effect of miR-212 mimic microinjection on abundance of FIGLA protein in 8-cell embryos as determined by immuofluorescent staining with anti-FIGLA antibody (n = 2 pools of 20 embryos per treatment). [score:1]
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2
[+] score: 7
Recently, it has been reported that overexpression of miR-212 protects against the development of cocaine addiction [35], pointing to a crucial role of miRNAs in the addiction process. [score:4]
Emerging evidence has revealed that miRNAs are also present in the CNS [32], [33], where cocaine administration alters the expression of many miRNAs (let-7d, miR-1, miR-124, miR-181a, miR-29b, miR-31, miR-382 and miR-212) in brain regions related to cocaine addiction (nucleus accumbens, ventral tegmental area, prefrontal cortex and dorsal striatum) [34], [35], [36]. [score:3]
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3
[+] score: 3
MicroRNA dysregulation has been associated with many tumour types and specifically miR-212 has been linked to lung cancer progression via its negative regulatory activity against the Ptc1 receptor [21]. [score:3]
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4
[+] score: 3
In this sense, it is known that different exogenous agents can induce the increase or decrease of specific miRNAs, for instance, cocaine administration alters the expression of many miRNAs (miR-1, miR-124, miR-181a, miR-29b, miR-31, miR-382, miR-212 and let-7d) in brain regions related to cocaine addiction (nucleus accumbens, ventral tegmental area, prefrontal cortex and dorsal striatum) [21], [64], [65]. [score:3]
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5
[+] score: 1
Other miRNAs from this paper: hsa-let-7a-1, hsa-let-7a-2, hsa-let-7a-3, hsa-let-7b, hsa-let-7e, hsa-mir-20a, hsa-mir-21, hsa-mir-23a, hsa-mir-24-1, hsa-mir-24-2, hsa-mir-26b, hsa-mir-27a, hsa-mir-29a, hsa-mir-31, hsa-mir-29b-1, hsa-mir-29b-2, hsa-mir-103a-2, hsa-mir-103a-1, hsa-mir-199a-1, hsa-mir-148a, hsa-mir-7-1, hsa-mir-7-2, hsa-mir-7-3, hsa-mir-10b, hsa-mir-181a-2, hsa-mir-181b-1, hsa-mir-181c, hsa-mir-199a-2, hsa-mir-199b, hsa-mir-203a, hsa-mir-204, hsa-mir-212, hsa-mir-181a-1, hsa-mir-221, hsa-mir-23b, hsa-mir-27b, hsa-mir-128-1, hsa-mir-132, hsa-mir-133a-1, hsa-mir-133a-2, hsa-mir-143, hsa-mir-200c, hsa-mir-181b-2, hsa-mir-128-2, hsa-mir-200a, hsa-mir-30e, hsa-mir-148b, hsa-mir-338, hsa-mir-133b, dre-mir-7b, dre-mir-7a-1, dre-mir-7a-2, dre-mir-10b-1, dre-mir-181b-1, dre-mir-181b-2, dre-mir-199-1, dre-mir-199-2, dre-mir-199-3, dre-mir-203a, dre-mir-204-1, dre-mir-181a-1, dre-mir-221, dre-mir-222a, dre-let-7a-1, dre-let-7a-2, dre-let-7a-3, dre-let-7a-4, dre-let-7a-5, dre-let-7a-6, dre-let-7b, dre-let-7e, dre-mir-7a-3, dre-mir-10b-2, dre-mir-20a, dre-mir-21-1, dre-mir-21-2, dre-mir-23a-1, dre-mir-23a-2, dre-mir-23a-3, dre-mir-23b, dre-mir-24-4, dre-mir-24-2, dre-mir-24-3, dre-mir-24-1, dre-mir-26b, dre-mir-27a, dre-mir-27b, dre-mir-29b-1, dre-mir-29b-2, dre-mir-29a, dre-mir-30e-2, dre-mir-101b, dre-mir-103, dre-mir-128-1, dre-mir-128-2, dre-mir-132-1, dre-mir-132-2, dre-mir-133a-2, dre-mir-133a-1, dre-mir-133b, dre-mir-133c, dre-mir-143, dre-mir-148, dre-mir-181c, dre-mir-200a, dre-mir-200c, dre-mir-203b, dre-mir-204-2, dre-mir-338-1, dre-mir-338-2, dre-mir-454b, hsa-mir-181d, dre-mir-181a-2, hsa-mir-551a, hsa-mir-551b, dre-mir-31, dre-mir-722, dre-mir-724, dre-mir-725, dre-mir-735, dre-mir-740, hsa-mir-103b-1, hsa-mir-103b-2, dre-mir-2184, hsa-mir-203b, dre-mir-7146, dre-mir-181a-4, dre-mir-181a-3, dre-mir-181a-5, dre-mir-181b-3, dre-mir-181d, dre-mir-204-3, dre-mir-24b, dre-mir-7133, dre-mir-128-3, dre-mir-7132, dre-mir-338-3
Although zebrafish miRNAs have been examined in numerous studies [25, 27, 41– 43], our analysis revealed novel paralogs of 18 miRNAs that do not currently have zebrafish records in miRBase (version 21), including miR-181a, miR-20a, miR-23b, miR-24, miR-29a, miR-103, miR-128, miR-148, miR-181b, miR-199, miR-204, miR-212, miR-221, miR-338, miR-724, miR-2184, let-7b and let-7e. [score:1]
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