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38 publications mentioning dre-mir-430i-2

Open access articles that are associated with the species Danio rerio and mention the gene name mir-430i-2. Click the [+] symbols to view sentences that include the gene name, or the word cloud on the right for a summary.

1
[+] score: 128
Our previous study showed that microRNA (miRNA) miR-430, which is abundantly expressed from the onset of zygotic gene expression [8], targets the 3′UTRs of nanos1 and tdrd7 to induce mRNA deadenylation, mRNA degradation, and translational repression in somatic cells [5]. [score:9]
Furthermore, DAZL activated the expression of GFP-IPT [miR-430] 91–123 mRNA that contains a portion of the tdrd7 3′UTR (corresponding to the nucleoteides +91–123 relative to the stop codon) fused with a single copy of the exogenous miR-430 target site (IPT [miR-430]) (4.1-fold, Fig. 2B). [score:5]
In contrast, GFP expression of a reporter mRNA (GFP-3xIPT [miR-430]6xmt2), containing miR-430 target sites with the mutant elements of #16 that do not bind to DAZL [15], was not largely affected by DAZL (1.0-fold, Fig. 3B). [score:5]
The miR-204 target site (IPT [miR204]) was inserted into the 3′UTR of the GFP- tdrd7 construct, in which the miR-430 target sites [5] had been disrupted by base substitutions (Fig. S2, panels A and B). [score:5]
Importantly, miR-430 is also expressed in PGCs, and other target mRNAs of miR-430 are equally susceptible to repression in somatic cells and PGCs [5], [9]. [score:5]
miR-204 is not strongly expressed during early embryogenesis and therefore we could mimic the ubiquitous expression of miR-430 by injecting miR-204 duplex [5]. [score:5]
Expression of the reporter mRNA, GFP-3xIPT [miR-430], containing three copies of synthetic miR-430 target site (IPT [miR-430]) that is derived from nanos1 3′UTR is completely repressed by miR-430 not only in somatic cells but also in PGCs [5], [9]. [score:5]
To further examine whether or not direct binding of DAZL to the cis-element is sufficient for the relief of miRNA -mediated repression, we tested a reporter mRNA, GFP-3xIPT [miR-430] 6x#16, containing three copies of the miR-430 target site fused with six copies of the in vitro selected DAZL -binding sequence, #16, that possessed the GUUC element [15] (Fig. 3A). [score:4]
The exogenous miR-430 target site (IPT [miR-430]) in the IPT [miR-430] 91–123 and IPT [miR-430] 91–123 mt constructs were derived from the nanos1 3′UTR [5]. [score:3]
These results showed that DAZL can activate protein synthesis through the 3′UTR of tdrd7 mRNA targeted by miR-430, and that the RRM and DAZ motifs are involved in the activation. [score:3]
DAZL -binding element is sufficient for the PGC-specific activation of miR-430 target mRNA. [score:3]
In zebrafish embryos, miR-430 enhances the degradation of target mRNAs [9]. [score:3]
In zebrafish and human germline cells, DND suppresses miR-430 function through blocking miRNA accessibility by binding to U-rich mRNA regions (URRs) [10]. [score:3]
We examined whether DAZL's effect is specific to the miR-430 target site in the context of tdrd7 3′UTR. [score:3]
We also show that DAZL enhances protein synthesis via the 3′UTR of dazl mRNA, another germline mRNA targeted by miR-430. [score:3]
Putative DAZL -binding sites (GUUC; red, GUUA; yellow), miR-430 target sites (blue), and mutated DAZL -binding sites (gray) are indicated. [score:3]
The tdrd7 3′UTR contains cis-elements for the activation by DAZL proteinWe examined whether DAZL's effect is specific to the miR-430 target site in the context of tdrd7 3′UTR. [score:3]
The loss of DND function or its target sequences make nanos and tdrd7 3′UTRs susceptible to miR-430 -mediated repression in zebrafish. [score:3]
We also show that DAZL can enhance protein synthesis via the 3′UTR of dazl mRNA which is localized to PGCs and targeted by miR-430. [score:3]
When we examined a reporter mRNA, GFP-IPT [miR-430] 91–123 mt, in which we had introduced the base substitutions into the GUUC and GUUA elements, GFP expression was not enhanced even in the presence of DAZL (1.1-fold, Fig. 2B). [score:3]
The target site of miR-204 (blue) and the mutated site for miR-430 (gray) are shown. [score:3]
During zebrafish embryogenesis, microRNA (miRNA) miR-430 contributes to restrict Nanos1 and TDRD7 to primordial germ cells (PGCs) by inducing mRNA deadenylation, mRNA degradation, and translational repression of nanos1 and tdrd7 mRNAs in somatic cells. [score:3]
When GFP- tdrd7 and DsRed mRNAs were injected, GFP expression was barely detectable in somatic cells at 24 hr post-fertilization (hpf), due to the repression by miR-430 [5] (Fig. 1A, panel e). [score:3]
Moreover, the addition of DAZL -binding elements to the synthetic miR-430 target mRNA led to mRNA stabilization in a PGC-specific manner in embryos. [score:3]
The dazl 3′UTR contains a putative miR-430 target site (corresponding to the nucleoteides +813–835 relative to the stop codon), which has the octamer sequence complementary for the miR-430 seed sequence. [score:3]
In situ hybridization of the embryos injected with GFP- tdrd7 mRNA was performed at 80%-epiboly (∼8 hpf), since miR-430 -mediated mRNA degradation can be observed immediately after the onset of zygotic expression (around 4–8 hpf) [5]. [score:3]
Sequences of wildtype and mutated miR-430 target sites are shown below. [score:3]
DAZL also controls another germline mRNA, dazl, targeted by miR-430. [score:3]
0007513.g005 Figure 5Regulation of dazl mRNA by miR-430 and DAZL. [score:2]
Regulation of dazl mRNA by miR-430 and DAZL. [score:2]
Taken together, we concluded that DAZL also counteracts miR-430 -mediated repression of dazl mRNA, by inducing polyadenylation. [score:1]
Nucleotides that basepair with miR-430 seed are indicated in blue. [score:1]
These results led us to conclude that DAZL binds to the cis-elements in the 3′UTR and cancels the miR-430 -mediated repression of tdrd7. [score:1]
In zebrafish PGCs, DND alleviates miR-430 repression of nanos1 and tdrd7. [score:1]
Using a GFP reporter mRNA that was fused with tdrd7 3′UTR, we show here that DAZL antagonizes miR-430 -mediated repression of the tdrd7 mRNA in zebrafish embryos. [score:1]
Thus, it is likely that DAZL and DND proteins function additively to exclude the miR-430 function on the various germline mRNAs. [score:1]
Sequences of IPT [miR-430] and #16 are shown below. [score:1]
In the present study, we assumed that another RNA -binding protein, DAZL, can also relieve the miR-430 -mediated repression of germ plasm mRNAs in zebrafish, for two reasons. [score:1]
Second, we measured the poly(A) tail length of a mutant GFP- tdrd7 mRNA that lacks the miR-430 target sites, which is not subjected to the repression mediated by miR-430 [5]. [score:1]
To determine whether miR-430 -mediated repression is relieved endogenously through the DAZL -binding sequence, we performed in situ hybridization of embryos injected with GFP-3xIPT [miR-430] 6x#16 mRNA. [score:1]
DAZL relieves the miR-430 -mediated repression via binding to the cis-element GUUC. [score:1]
Thus miR-430 -mediated repression and the activation of germ plasm mRNAs play important roles in germline/somatic cell distinctions in zebrafish embryos. [score:1]
0007513.g003 Figure 3DAZL relieves the miR-430 -mediated repression via binding to the cis-element GUUC. [score:1]
So we examined if dazl mRNA is also controlled by a combination of DAZL and miR-430. [score:1]
The second is that DAZL blocks miR-430 function on tdrd7 mRNA or its access to tdrd7 mRNA, and thereby tdrd7 mRNA is free from the deadenylation mediated by miR-430. [score:1]
Nucleotides that are complementary with miR-430 seed are shown in blue. [score:1]
The results did not support the second possibility that DAZL only blocks deadenylation mediated by miR-430. [score:1]
Using a GFP reporter mRNA that was fused with tdrd7 3′UTR, we show that a germline-specific RNA -binding protein DAZ-like (DAZL) can relieve the miR-430 -mediated repression of tdrd7 mRNA by inducing poly(A) tail elongation (polyadenylation) in zebrafish. [score:1]
The sequence that basepairs with miR-430 seed (blue) and the DAZL -binding sequence (red) are indicated. [score:1]
These results suggested that endogenous DAZL protein cancels the repressive effect of miR-430 on tdrd7 mRNA in PGCs. [score:1]
As a result, the mRNA was detected in a PGC-specific manner in the absence of exogenous DAZL, while GFP-3xIPT [miR-430]+6xmt2 mRNA was undetectable throughout the embryo (Fig. 3C). [score:1]
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2
[+] score: 48
Surprisingly, our microarray -based analysis of miRNA expression in lin-28a and lin-28b morphants revealed that the expression of miR-430 family miRNAs was significantly downregulated in these morphants. [score:8]
These results suggest the existence of an evolutionarily conserved heterochronic gene regulatory network in vertebrate development, and a possible correlation between the expression of lin-28 genes and the miR-430 family, which is a key regulator of maternal mRNA clearance. [score:6]
Interestingly, a microarray -based analysis of miRNAs showed that miR-430 expression was inhibited in lin-28a and lin-28b morphant embryos, suggesting that the clearance of maternal mRNAs was affected in these morphants. [score:5]
Lin-28a and Lin-28b Regulate the Expression of Downstream Heterochronic Genes, as well as the miR-430 miRNA familyIn the C. elegans heterochronic pathway, miRNAs, including let-7 and lin-4/miR-125, play a critical role as downstream genes of lin28. [score:4]
Interestingly, we found a marked decrease in the expression of members of the miR-430 family, which was recently demonstrated to play a crucial role in deadenylation and clearance of maternal mRNAs during early zebrafish development (Table 1). [score:4]
Lin-28a and Lin-28b Regulate the Expression of Downstream Heterochronic Genes, as well as the miR-430 miRNA family. [score:4]
B. A schematic diagram summarizing heterochronic gene and miR-430 expression during zebrafish development. [score:4]
Moreover, consistent with the timing of zygotic transcription, miR-430 expression was activated, and its levels rose. [score:3]
Since miR-430 can rescue the severe developmental defects in MZ dicer mutant embryos, miR-430 is suggested to play a central role in early development [12]. [score:3]
Interestingly, the injection of miR-430, which has a crucial function in deadenylation and the clearance of maternal mRNAs, rescues these brain defects, suggesting a critical role for miR-430 in early zebrafish development [12]. [score:2]
Thus, we speculate that the function of Lin-28 during early zebrafish development involves the miR-430 -mediated clearance of maternal mRNAs. [score:2]
In the early development of zebrafish, miR-430 accumulates during the maternal-to-zygotic transition and promotes deadenylation and the clearance of hundreds of maternal mRNAs [12]. [score:2]
Currently, we do not have an explanation for the genetic interaction between lin-28 and miR-430. [score:1]
[1 to 20 of 13 sentences]
3
[+] score: 26
In zebrafish, the microRNA miR-430 is expressed at the onset of zygotic transcription and is responsible for the downregulation of several hundred target mRNAs, most of which are maternally deposited messages, through deadenylation and clearance of these mRNAs [9]. [score:8]
miR-430 has been shown to directly target and degrade the nanos, TDRD7 and dazl messages in the zebrafish soma [6], [8]. [score:4]
The elucidation of the mechanisms of germ cell specific expression of the HuB mRNA is an important finding, for it reveals mechanisms of post-transcriptional regulation that are distinct from that of the two best understood germ cell specific mRNAs, nanos and TDRD7, in which DND and DAZL can relieve miR-430 -mediated repression of these messages in germ cells [8], [10]. [score:4]
DND protects nanos and TDRD7 mRNAs from degradation in the germ cells by binding to U-rich sequence elements in each 3′UTR and inhibiting the activity of miR-430 [10]. [score:3]
Indeed, the germ cell specific vasa and dnd messages are not targeted by miR-430 [3], [6], [7]; nothing is yet known about how these two mRNAs are degraded in the somatic tissue. [score:3]
While the microRNA miR-430 is responsible for promoting the deadenylation and subsequent degradation of several germ cell specific mRNAs in the somatic tissue, a canonical miR-430 binding site is not present in the HuB 3′UTR [6], [9]. [score:1]
While it is possible that our A RNA deletion reporter, which would lack the three 5′ non-canonical miR-430 sites, is more stable than the full-length HuB 3′UTR, it is clear from our results that the most important elements controlling differential stability in the soma and in the germ cells reside in the last 144 nt of the HuB 3′UTR. [score:1]
For example, vasa [3], [6] and dnd [7] do not contain canonical miR-430 binding sites, suggesting that these mRNAs may be localised to the germ cells through unique post-transcriptional mechanisms. [score:1]
MicroRNA miR-430 is known to promote the deadenylation and clearance of other germ cell specific mRNAs, such as nanos, TDRD7, and dazl, in the somatic tissue of zebrafish [6], [8], however a canonical miR-430 binding site is not present in the HuB 3′UTR. [score:1]
[1 to 20 of 9 sentences]
4
[+] score: 25
Customized TALE repeats fused to a FokI nuclease were assembled using the Golden Gate method 13, and engineered to target 20 bp and 18 bp flanking the miR-430 target site, with a 16-bp spacer (Fig. 1a,b). [score:5]
miR430 -mediated regulation of lft2 expression in zebrafish. [score:4]
Finally, sequence analysis of the target region demonstrated discrete in del mutations across the predicted miR-430 MRE (Fig. 1h). [score:4]
Analysis by in situ hybridization and quantitative PCR (qPCR) of single experimental embryos showed that lft2 expression level at shield stage was significantly increased as compared with controls (n=6, average lft2 increase significance P<0.005, Fig. 1c,d), suggesting de-repression of miR-430 -mediated regulation. [score:3]
Repeat variable di-residue (RVD) arrays were designed to target 20 and 18 nt flanking the lft2 3′UTR at the miR-430 -binding site, separated by a 16-nt spacer. [score:3]
A 408-bp fragment spanning the miR-430 target site was amplified from wild-type or TALEN -injected zebrafish gDNA using Phusion DNA polymerase (NEB, #M0532) and flanking primers lfty2-Fwd (5′-CCCATGATGTACCTGGTCAAAA-3′) and lfty2-Rev (5′-GCTGTGGTGACCCCTAATGAAT-3′). [score:2]
In zebrafish, morpholino TP oligonucleotides have been used to block a predicted miR-430 site in the lefty2 (lft2) 3′UTR, causing a midline development phenotype in the embryo as a consequence of impaired Nodal signalling 8. To provide proof of concept for our approach, we first designed a TALEN pair to genetically ablate this miR-430 site in lft2 DNA (Fig. 1a,b). [score:2]
Disruption of the lft2 miR-430 MRE genomic locus was confirmed in TALEN -injected embryos, by the loss of Bsp1286I restriction site (Fig. 1e). [score:1]
The spacer region includes a Bsp1286I restriction enzyme-recognition sequence, which overlaps the predicted TALEN cut site, and the seed region of the miR-430 MRE. [score:1]
[1 to 20 of 9 sentences]
5
[+] score: 25
Other miRNAs from this paper: dre-mir-196a-1, dre-mir-199-1, dre-mir-199-2, dre-mir-199-3, dre-mir-203a, dre-mir-210, dre-mir-214, dre-mir-219-1, dre-mir-219-2, dre-mir-221, dre-mir-222a, dre-mir-430a-1, dre-mir-430b-1, dre-mir-430c-1, dre-mir-429a, dre-let-7a-1, dre-let-7a-2, dre-let-7a-3, dre-let-7a-4, dre-let-7a-5, dre-let-7a-6, dre-let-7b, dre-let-7c-1, dre-let-7c-2, dre-let-7d-1, dre-let-7d-2, dre-let-7e, dre-let-7f, dre-let-7g-1, dre-let-7g-2, dre-let-7h, dre-let-7i, dre-mir-1-2, dre-mir-1-1, dre-mir-9-1, dre-mir-9-2, dre-mir-9-4, dre-mir-9-3, dre-mir-9-5, dre-mir-9-6, dre-mir-9-7, dre-mir-21-1, dre-mir-21-2, dre-mir-25, dre-mir-30e-2, dre-mir-101a, dre-mir-103, dre-mir-107a, dre-mir-122, dre-mir-124-1, dre-mir-124-2, dre-mir-124-3, dre-mir-124-4, dre-mir-124-5, dre-mir-124-6, dre-mir-126a, dre-mir-129-2, dre-mir-129-1, dre-mir-130b, dre-mir-130c-1, dre-mir-130c-2, dre-mir-133a-2, dre-mir-133a-1, dre-mir-133b, dre-mir-133c, dre-mir-135c-1, dre-mir-135c-2, dre-mir-140, dre-mir-142a, dre-mir-142b, dre-mir-150, dre-mir-152, dre-mir-462, dre-mir-196a-2, dre-mir-196b, dre-mir-202, dre-mir-203b, dre-mir-219-3, dre-mir-365-1, dre-mir-365-2, dre-mir-365-3, dre-mir-455-1, dre-mir-430c-2, dre-mir-430c-3, dre-mir-430c-4, dre-mir-430c-5, dre-mir-430c-6, dre-mir-430c-7, dre-mir-430c-8, dre-mir-430c-9, dre-mir-430c-10, dre-mir-430c-11, dre-mir-430c-12, dre-mir-430c-13, dre-mir-430c-14, dre-mir-430c-15, dre-mir-430c-16, dre-mir-430c-17, dre-mir-430c-18, dre-mir-430a-2, dre-mir-430a-3, dre-mir-430a-4, dre-mir-430a-5, dre-mir-430a-6, dre-mir-430a-7, dre-mir-430a-8, dre-mir-430a-9, dre-mir-430a-10, dre-mir-430a-11, dre-mir-430a-12, dre-mir-430a-13, dre-mir-430a-14, dre-mir-430a-15, dre-mir-430a-16, dre-mir-430a-17, dre-mir-430a-18, dre-mir-430i-1, dre-mir-430i-3, dre-mir-430b-2, dre-mir-430b-3, dre-mir-430b-4, dre-mir-430b-6, dre-mir-430b-7, dre-mir-430b-8, dre-mir-430b-9, dre-mir-430b-10, dre-mir-430b-11, dre-mir-430b-12, dre-mir-430b-13, dre-mir-430b-14, dre-mir-430b-15, dre-mir-430b-16, dre-mir-430b-17, dre-mir-430b-18, dre-mir-430b-5, dre-mir-430b-19, dre-mir-430b-20, dre-let-7j, dre-mir-135b, dre-mir-135a, dre-mir-499, dre-mir-738, dre-mir-429b, dre-mir-1788, dre-mir-196c, dre-mir-107b, dre-mir-455-2, dre-mir-222b, dre-mir-126b, dre-mir-196d, dre-mir-129-3, dre-mir-129-4
For example, the miR-430 family, dre-miR-135c and dre-miR-9 were mainly expressed during development, but the miR-430 family was absent in adult fish, while dre-miR-135c and dre-miR-9 showed decreased expression in mature organs with exception of the brain. [score:6]
Dre-miR-133 expression was higher in muscle and dre-miR-430 showed higher expression in developmental samples. [score:6]
Some miRNAs were mainly expressed during development, namely dre-miR-430 family, dre-miR-135c and dre-miR-9. The former was absent in the adult fish while dre-miR-135c and dre-miR-9 had decreased expression in mature organs with exception of the brain. [score:6]
The expression profile also highlighted results of Giraldez and colleagues [39] showing that miR-430 is essential for regulation of morphogenesis during development. [score:5]
The miR-430 family was also present during development and was absent in adult ZF [19, 20]. [score:2]
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6
[+] score: 19
Translational inhibition of transcripts carrying DLEs could, potentially, also arise from reduced mRNA stability, as shown for miR-430 mediated repression and mRNA decay (62, 64). [score:5]
Choi W. Y., Giraldez A. J., Schier A. F. Target protectors reveal dampening and balancing of Nodal agonist and antagonist by miR-430. [score:3]
Post-transcriptional regulation of the Nodal pathway through the small regulatory RNA miR-430 was reported to dampen and balance Nodal signaling by mRNA degradation (61). [score:3]
Bazzini A. A., Lee M. T., Giraldez A. J. Ribosome profiling shows that miR-430 reduces translation before causing mRNA decay in zebrafish. [score:3]
Mishima Y., Giraldez A. J., Takeda Y., Fujiwara T., Sakamoto H., Schier A. F., Inoue K. Differential regulation of germline mRNAs in soma and germ cells by zebrafish miR-430. [score:2]
However, miR430 is not active at the earliest stages of zebrafish development, prior to its transcription from the zygotic genome (62). [score:2]
Maternally provided proteins deposited in the egg, such as Ybx1, control RNAs (including Nodal pathway transcripts) in early embryos prior to miR-430 function (63). [score:1]
[1 to 20 of 7 sentences]
7
[+] score: 13
Other miRNAs from this paper: hsa-mir-23a, hsa-mir-29a, hsa-mir-29b-1, hsa-mir-29b-2, hsa-mir-107, hsa-mir-205, hsa-mir-214, hsa-mir-221, hsa-mir-1-2, hsa-mir-122, hsa-mir-133a-1, hsa-mir-133a-2, hsa-mir-184, hsa-mir-193a, hsa-mir-1-1, hsa-mir-29c, hsa-mir-133b, dre-mir-205, dre-mir-214, dre-mir-221, dre-mir-430a-1, dre-mir-430b-1, dre-mir-430c-1, dre-mir-1-2, dre-mir-1-1, dre-mir-23a-1, dre-mir-23a-2, dre-mir-23a-3, dre-mir-29b-1, dre-mir-29b-2, dre-mir-29a, dre-mir-107a, dre-mir-122, dre-mir-133a-2, dre-mir-133a-1, dre-mir-133b, dre-mir-133c, dre-mir-184-1, dre-mir-193a-1, dre-mir-193a-2, dre-mir-202, dre-mir-430c-2, dre-mir-430c-3, dre-mir-430c-4, dre-mir-430c-5, dre-mir-430c-6, dre-mir-430c-7, dre-mir-430c-8, dre-mir-430c-9, dre-mir-430c-10, dre-mir-430c-11, dre-mir-430c-12, dre-mir-430c-13, dre-mir-430c-14, dre-mir-430c-15, dre-mir-430c-16, dre-mir-430c-17, dre-mir-430c-18, dre-mir-430a-2, dre-mir-430a-3, dre-mir-430a-4, dre-mir-430a-5, dre-mir-430a-6, dre-mir-430a-7, dre-mir-430a-8, dre-mir-430a-9, dre-mir-430a-10, dre-mir-430a-11, dre-mir-430a-12, dre-mir-430a-13, dre-mir-430a-14, dre-mir-430a-15, dre-mir-430a-16, dre-mir-430a-17, dre-mir-430a-18, dre-mir-430i-1, dre-mir-430i-3, dre-mir-430b-2, dre-mir-430b-3, dre-mir-430b-4, dre-mir-430b-6, dre-mir-430b-7, dre-mir-430b-8, dre-mir-430b-9, dre-mir-430b-10, dre-mir-430b-11, dre-mir-430b-12, dre-mir-430b-13, dre-mir-430b-14, dre-mir-430b-15, dre-mir-430b-16, dre-mir-430b-17, dre-mir-430b-18, dre-mir-430b-5, dre-mir-430b-19, dre-mir-430b-20, hsa-mir-202, hsa-mir-499a, dre-mir-184-2, dre-mir-499, dre-mir-724, dre-mir-725, dre-mir-107b, dre-mir-2189, hsa-mir-499b, dre-mir-29b3
A detailed analysis of predicted miR-430 targets revealed that more than 40% of these targets were maternal transcripts that were degraded at MZT [149], highlighting that miR-430 acts as a “developmental switch” by clearing maternal transcripts to facilitate the transition to zygotic programs. [score:6]
The zebrafish miR-430 family, which is first expressed during maternal to zygotic transition (MZT), is the most abundant miRNA family during early embryogenesis [148], and has been shown to be essential during zebrafish development [149, 150] with striking impacts on brain morphogenesis [151]. [score:4]
Dre-miR-430a and dre-miR-133a have more targets in common than with any other miR-430 family members. [score:3]
[1 to 20 of 3 sentences]
8
[+] score: 12
Other miRNAs from this paper: hsa-let-7a-1, hsa-let-7a-2, hsa-let-7a-3, hsa-let-7b, hsa-let-7c, hsa-let-7d, hsa-let-7e, hsa-let-7f-1, hsa-let-7f-2, hsa-mir-21, hsa-mir-148a, hsa-let-7g, hsa-let-7i, hsa-mir-122, hsa-mir-34c, hsa-mir-148b, dre-mir-430a-1, dre-mir-430b-1, dre-mir-430c-1, dre-let-7a-1, dre-let-7a-2, dre-let-7a-3, dre-let-7a-4, dre-let-7a-5, dre-let-7a-6, dre-let-7b, dre-let-7c-1, dre-let-7c-2, dre-let-7d-1, dre-let-7d-2, dre-let-7e, dre-let-7f, dre-let-7g-1, dre-let-7g-2, dre-let-7h, dre-let-7i, dre-mir-10c, dre-mir-21-1, dre-mir-21-2, dre-mir-122, dre-mir-135c-1, dre-mir-135c-2, dre-mir-148, dre-mir-430c-2, dre-mir-430c-3, dre-mir-430c-4, dre-mir-430c-5, dre-mir-430c-6, dre-mir-430c-7, dre-mir-430c-8, dre-mir-430c-9, dre-mir-430c-10, dre-mir-430c-11, dre-mir-430c-12, dre-mir-430c-13, dre-mir-430c-14, dre-mir-430c-15, dre-mir-430c-16, dre-mir-430c-17, dre-mir-430c-18, dre-mir-430a-2, dre-mir-430a-3, dre-mir-430a-4, dre-mir-430a-5, dre-mir-430a-6, dre-mir-430a-7, dre-mir-430a-8, dre-mir-430a-9, dre-mir-430a-10, dre-mir-430a-11, dre-mir-430a-12, dre-mir-430a-13, dre-mir-430a-14, dre-mir-430a-15, dre-mir-430a-16, dre-mir-430a-17, dre-mir-430a-18, dre-mir-430i-1, dre-mir-430i-3, dre-mir-430b-2, dre-mir-430b-3, dre-mir-430b-4, dre-mir-430b-6, dre-mir-430b-7, dre-mir-430b-8, dre-mir-430b-9, dre-mir-430b-10, dre-mir-430b-11, dre-mir-430b-12, dre-mir-430b-13, dre-mir-430b-14, dre-mir-430b-15, dre-mir-430b-16, dre-mir-430b-17, dre-mir-430b-18, dre-mir-430b-5, dre-mir-430b-19, dre-mir-430b-20, dre-mir-459, hsa-mir-499a, dre-let-7j, dre-mir-499, dre-mir-34c, dre-mir-734, hsa-mir-499b, dre-mir-7146, dre-mir-7147, dre-mir-7148
The miR-430 family was found to be very highly expressed in embryo with very low to none expression in adult tissues as seen in previous studies [18]. [score:5]
The expression of miR-430 family peaks at the blastula stage (4 h) and dominates the miRNA profile up to the pharyngula stage (24 h) and then decreases. [score:3]
The dre-miR-430 miRNA family was highly enriched in embryo [20] was also detected by us. [score:1]
The miR-430 family has been shown to be involved in maternal RNA degradation during gastrulation [19]. [score:1]
Role of miR-430 in maternal RNA clearance during maternal to zygotic transition has been well documented [19]. [score:1]
Since our samples contained gastrulation stage embryo as well, it is logical to expect abundant presence of miR-430 in the sequence data. [score:1]
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9
[+] score: 12
Other miRNAs from this paper: mmu-let-7g, mmu-let-7i, mmu-mir-124-3, mmu-mir-140, mmu-mir-141, mmu-mir-152, mmu-mir-182, mmu-mir-183, mmu-mir-191, mmu-mir-199a-1, mmu-mir-200b, mmu-mir-205, mmu-let-7d, mmu-mir-200a, mmu-let-7a-1, mmu-let-7a-2, mmu-let-7b, mmu-let-7c-1, mmu-let-7c-2, mmu-let-7e, mmu-let-7f-1, mmu-let-7f-2, mmu-mir-96, mmu-mir-200c, mmu-mir-214, mmu-mir-199a-2, mmu-mir-199b, mmu-mir-124-1, mmu-mir-124-2, mmu-mir-7a-1, mmu-mir-7a-2, mmu-mir-7b, dre-mir-7b, dre-mir-7a-1, dre-mir-7a-2, dre-mir-182, dre-mir-183, dre-mir-199-1, dre-mir-199-2, dre-mir-199-3, dre-mir-205, dre-mir-214, dre-mir-430a-1, dre-mir-430b-1, dre-mir-430c-1, mmu-mir-429, mmu-mir-449a, dre-mir-429a, dre-let-7a-1, dre-let-7a-2, dre-let-7a-3, dre-let-7a-4, dre-let-7a-5, dre-let-7a-6, dre-let-7b, dre-let-7c-1, dre-let-7c-2, dre-let-7d-1, dre-let-7d-2, dre-let-7e, dre-let-7f, dre-let-7g-1, dre-let-7g-2, dre-let-7h, dre-let-7i, dre-mir-7a-3, dre-mir-96, dre-mir-124-1, dre-mir-124-2, dre-mir-124-3, dre-mir-124-4, dre-mir-124-5, dre-mir-124-6, dre-mir-140, dre-mir-141, dre-mir-152, dre-mir-200a, dre-mir-200b, dre-mir-200c, dre-mir-430c-2, dre-mir-430c-3, dre-mir-430c-4, dre-mir-430c-5, dre-mir-430c-6, dre-mir-430c-7, dre-mir-430c-8, dre-mir-430c-9, dre-mir-430c-10, dre-mir-430c-11, dre-mir-430c-12, dre-mir-430c-13, dre-mir-430c-14, dre-mir-430c-15, dre-mir-430c-16, dre-mir-430c-17, dre-mir-430c-18, dre-mir-430a-2, dre-mir-430a-3, dre-mir-430a-4, dre-mir-430a-5, dre-mir-430a-6, dre-mir-430a-7, dre-mir-430a-8, dre-mir-430a-9, dre-mir-430a-10, dre-mir-430a-11, dre-mir-430a-12, dre-mir-430a-13, dre-mir-430a-14, dre-mir-430a-15, dre-mir-430a-16, dre-mir-430a-17, dre-mir-430a-18, dre-mir-430i-1, dre-mir-430i-3, dre-mir-430b-2, dre-mir-430b-3, dre-mir-430b-4, dre-mir-430b-6, dre-mir-430b-7, dre-mir-430b-8, dre-mir-430b-9, dre-mir-430b-10, dre-mir-430b-11, dre-mir-430b-12, dre-mir-430b-13, dre-mir-430b-14, dre-mir-430b-15, dre-mir-430b-16, dre-mir-430b-17, dre-mir-430b-18, dre-mir-430b-5, dre-mir-430b-19, dre-mir-430b-20, dre-let-7j, mmu-mir-449c, mmu-mir-449b, dre-mir-429b, mmu-let-7j, mmu-let-7k, mmu-mir-124b
Injection of miR-430 into MZ dicer mutants rescues early abnormalities, but does not restore the function of microRNAs that are expressed at later stages of development. [score:4]
However, in contrast to control animals, the expression of markers of terminally differentiated olfactory sensory neurons, such as OMP and olfactory receptors, is largely abolished in MZ dicer [+miR-430] mutants at 48 hpf (Figure 7B). [score:3]
We therefore analyzed olfactory development in MZ dicer mutants injected with miR-430 microRNAs. [score:2]
The similarity in the cellular and molecular process of olfactory development in zebrafish and mouse and the parallel olfactory defects observed in MZ dicer [+miR-430] zebrafish embryos and in Foxg1-Cre; Dicer [loxP/loxP] mouse embryos allowed us to use an antisense morpholino -mediated strategy (Flynt et al., 2007; Kloosterman et al., 2007). [score:2]
Early patterning of the nervous system is unperturbed in MZ dicer [+miR430] mutants, e. g., markers for specified optic stalk, forebrain, midbrain-hindbrain boundary, otic vesicles, hindbrain rhombomeres, dorsal neural tube, and ventral neural tube are present (Giraldez et al., 2005). [score:1]
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10
[+] score: 11
Such large copy numbers are consistent with the function of the miR-430 family to target and degrade the wide variety and number of maternally deposited mRNAs coincident with zygotic transcription at the midblastula transition [22]. [score:3]
The largest of these families (the miR-430 family) contains 72 members largely clustered in two main locations along chromosome 4. Thus far, most zebrafish miRNAs exhibit tissue specific patterns of expression. [score:3]
For example, miR-214 is required for proper muscle formation, miR-375 is needed for pancreatic islet development, and the large miR-430 family is needed for deadenylation and clearance of maternal mRNAs at the midblastula transition [21- 23]. [score:2]
Strikingly, the miR-430 family contains two large clusters of 10 and 57 genes on chromosome 4 (Figure 2). [score:1]
As above, the best example of this is the miR-430 family which is the most abundant zebrafish miRNA discovered thus far. [score:1]
The miR-430 family has two large clusters on chromosome 4 consisting of 57 and 10 copies, respectively. [score:1]
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11
[+] score: 11
High concentrations of miRNA mimics have previously been shown to induce off-target effects with similar morphological defects through non-specific downregulation of miR-430 activity. [score:6]
The number of embryos presented with short size, heart edema or curved tail were reduced in the presence of miR-430 -mimics, suggesting that these phenotypes were most likely due to suppression of miR-430 activity. [score:3]
All MOs and synthetic miRNA sequences used in this study are listed in Supplementary Table 1. miR-430 mimics was obtained from Ambion (product ID MC10393) and was co -injected along with miR-137 mimics at 100 pg. [score:1]
[47] To test this hypothesis, we co -injected miR-137 -mimics along with miR-430 mimics (Figure 3h). [score:1]
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12
[+] score: 11
In zebrafish, miR-430 is a miRNA responsible for the downregulation of several hundred targeted mRNAs in soma cells including nanos. [score:6]
By binding to U-rich sequence elements in 3′ UTR of those mRNAs and inhibiting the activity of miR-430, dead end protein protects those mRNAs from degradation in the germ cells, thus, relieving miRNA repression in germline cells by blocking the accessibility of target mRNAs to miRNAs. [score:5]
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[+] score: 9
Thus, this differential post-transcriptional regulation of the two bmi1 onhologs potentially by the micro RNA miR-430, illustrates that various zebrafish pcgf genes are subjected to distinct expression controls during development. [score:5]
Zebrafish microRNA-430 (miR-430) family is highly expressed during early development. [score:4]
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[+] score: 9
So the high expression of miR-430 family here may be the organism's strategy to rescue the impairment or to balance the disorders of miRNAs and their target system caused by MC-RR. [score:5]
In the present studies, expression changes in miR-430 and miR-125 families were quite significant. [score:3]
Giraldez et al. [18] observed that injection of miR-430 rescues the early morphogenesis defects in dicer mutants. [score:1]
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[+] score: 8
miRsmn1-5 -expressing animals were injected with either hsa-SMN1 RNA or miR-430 to test their capacity to rescue the observed motoneuron phenotype. [score:3]
RNA and miR-430 mimics injections. [score:1]
A synthetic form of miR-430 was injected at ∼100–150 pg. [score:1]
When appropriate, the embryos were injected with RNA or miR-430 mimics (one-cell to four-cell stage). [score:1]
RNA and miR-430 mimics injectionsAll pME -RNAi constructs were linearized with BspHI and purified before reaction. [score:1]
miR-430 mimics were obtained from Ambion (product ID MC10393) and were injected between 100 and 150 pg depending on the experiment. [score:1]
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[+] score: 8
Other miRNAs from this paper: dre-mir-10a, dre-mir-10b-1, dre-mir-183, dre-mir-430a-1, dre-mir-430b-1, dre-mir-430c-1, dre-let-7a-1, dre-let-7a-2, dre-let-7a-3, dre-let-7a-4, dre-let-7a-5, dre-let-7a-6, dre-mir-1-2, dre-mir-9-1, dre-mir-9-2, dre-mir-9-4, dre-mir-9-3, dre-mir-9-5, dre-mir-9-6, dre-mir-9-7, dre-mir-10b-2, dre-mir-10c, dre-mir-10d, dre-mir-15a-1, dre-mir-15a-2, dre-mir-17a-1, dre-mir-17a-2, dre-mir-20a, dre-mir-29b-1, dre-mir-29b-2, dre-mir-29a, dre-mir-92a-1, dre-mir-92a-2, dre-mir-92b, dre-mir-101a, dre-mir-101b, dre-mir-124-1, dre-mir-124-2, dre-mir-124-3, dre-mir-124-4, dre-mir-124-5, dre-mir-124-6, dre-mir-145, dre-mir-430c-2, dre-mir-430c-3, dre-mir-430c-4, dre-mir-430c-5, dre-mir-430c-6, dre-mir-430c-7, dre-mir-430c-8, dre-mir-430c-9, dre-mir-430c-10, dre-mir-430c-11, dre-mir-430c-12, dre-mir-430c-13, dre-mir-430c-14, dre-mir-430c-15, dre-mir-430c-16, dre-mir-430c-17, dre-mir-430c-18, dre-mir-430a-2, dre-mir-430a-3, dre-mir-430a-4, dre-mir-430a-5, dre-mir-430a-6, dre-mir-430a-7, dre-mir-430a-8, dre-mir-430a-9, dre-mir-430a-10, dre-mir-430a-11, dre-mir-430a-12, dre-mir-430a-13, dre-mir-430a-14, dre-mir-430a-15, dre-mir-430a-16, dre-mir-430a-17, dre-mir-430a-18, dre-mir-430i-1, dre-mir-430i-3, dre-mir-430b-2, dre-mir-430b-3, dre-mir-430b-4, dre-mir-430b-6, dre-mir-430b-7, dre-mir-430b-8, dre-mir-430b-9, dre-mir-430b-10, dre-mir-430b-11, dre-mir-430b-12, dre-mir-430b-13, dre-mir-430b-14, dre-mir-430b-15, dre-mir-430b-16, dre-mir-430b-17, dre-mir-430b-18, dre-mir-430b-5, dre-mir-430b-19, dre-mir-430b-20, dre-mir-499, ola-mir-430a-1, ola-mir-430c-1, ola-mir-430b-1, ola-mir-430c-2, ola-mir-430c-3, ola-mir-430d-1, ola-mir-430a-2, ola-mir-430c-4, ola-mir-430d-2, ola-mir-430a-3, ola-mir-430a-4, ola-mir-430c-5, ola-mir-430d-3, ola-mir-430b-2, ola-mir-430c-6, ola-mir-430c-7, ola-mir-20a-1, ola-mir-92a-2, ola-mir-9a-2, ola-mir-101a, ola-mir-9b-1, ola-mir-499, ola-let-7a-1, ola-mir-9a-3, ola-mir-183-1, ola-let-7a-2, ola-mir-29b-1, ola-mir-29a, ola-mir-124-1, ola-mir-124-2, ola-mir-9a-4, ola-mir-101b, ola-let-7a-4, ola-mir-10d, ola-mir-9a-1, ola-mir-92b, ola-mir-9b-2, ola-mir-1-2, ola-mir-124-3, ola-mir-15a, ola-mir-10b, ola-mir-92a-1, ola-mir-20a-2, ola-mir-17, ola-mir-29b-2, ola-mir-29c, ola-mir-183-2, ola-let-7a-3, ola-mir-9a-5, ola-mir-145, dre-mir-29b3
It could be possible that multiple loci are required to increase the copy-number and therefore the expression level of specific miRNAs in particular conditions, like miR-430 in the maternal-zygotic transition in zebrafish (Danio rerio) [19]. [score:3]
Not only the number of miR-430 copies within each cluster varies greatly but also the number and organization of the members of this miRNA family. [score:1]
However, unusually large clusters were also found in some species, like the miR-430 cluster in zebrafish, consisting of 57 miRNAs [41, 61, 68]. [score:1]
c Structure comparison of the miR-430 cluster. [score:1]
However, a clear duplication pattern can be observed for the miR-430 cluster in D. rerio (the order miR-430c/b/a is repeated with only a few exceptions) and N. furzeri (the order miR-430c/a/a/c/a/a/a is repeated). [score:1]
For O. latipes and G. aculeatus, the order of miR-430 variants appears to be more random, and T. rubripes has too few copies to show any repeated pattern. [score:1]
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[+] score: 7
The increased expression level of miR-196a near the eight-cell stage of embryogenesis potentially indicates miR-196a involvement in maternal transcript degradation during the maternal-to-zygotic transition, as was observed for miR-430 in zebrafish [10] miR-427 in Xenopus [38] and miR-290 in mouse [20]. [score:3]
At the onset of embryonic genome activation, the level of miR-430 substantially increases and the miRNA targets several hundred maternally provided mRNAs by binding to the complementary sites in their 3' UTR and promotes their deadenylation [10]. [score:3]
In zebrafish, miR-430 has been linked to maternal mRNA decay accompanying the maternal-to-embryonic transition [10]. [score:1]
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[+] score: 7
Other miRNAs from this paper: dre-mir-7b, dre-mir-7a-1, dre-mir-7a-2, dre-mir-34a, dre-mir-181b-1, dre-mir-181b-2, dre-mir-182, dre-mir-183, dre-mir-181a-1, dre-mir-219-1, dre-mir-219-2, dre-mir-221, dre-mir-222a, dre-mir-430a-1, dre-mir-430b-1, dre-mir-430c-1, dre-let-7a-1, dre-let-7a-2, dre-let-7a-3, dre-let-7a-4, dre-let-7a-5, dre-let-7a-6, dre-let-7b, dre-let-7c-1, dre-let-7c-2, dre-let-7d-1, dre-let-7d-2, dre-let-7e, dre-let-7f, dre-let-7g-1, dre-let-7g-2, dre-let-7h, dre-let-7i, dre-mir-7a-3, dre-mir-9-1, dre-mir-9-2, dre-mir-9-4, dre-mir-9-3, dre-mir-9-5, dre-mir-9-6, dre-mir-9-7, dre-mir-92b, dre-mir-96, dre-mir-100-1, dre-mir-100-2, dre-mir-124-1, dre-mir-124-2, dre-mir-124-3, dre-mir-124-4, dre-mir-124-5, dre-mir-124-6, dre-mir-125b-1, dre-mir-125b-2, dre-mir-125b-3, dre-mir-128-1, dre-mir-128-2, dre-mir-132-1, dre-mir-132-2, dre-mir-135c-1, dre-mir-135c-2, dre-mir-137-1, dre-mir-137-2, dre-mir-138-1, dre-mir-153a, dre-mir-181c, dre-mir-200a, dre-mir-218a-1, dre-mir-218a-2, dre-mir-219-3, dre-mir-375-1, dre-mir-375-2, dre-mir-454a, dre-mir-430c-2, dre-mir-430c-3, dre-mir-430c-4, dre-mir-430c-5, dre-mir-430c-6, dre-mir-430c-7, dre-mir-430c-8, dre-mir-430c-9, dre-mir-430c-10, dre-mir-430c-11, dre-mir-430c-12, dre-mir-430c-13, dre-mir-430c-14, dre-mir-430c-15, dre-mir-430c-16, dre-mir-430c-17, dre-mir-430c-18, dre-mir-430a-2, dre-mir-430a-3, dre-mir-430a-4, dre-mir-430a-5, dre-mir-430a-6, dre-mir-430a-7, dre-mir-430a-8, dre-mir-430a-9, dre-mir-430a-10, dre-mir-430a-11, dre-mir-430a-12, dre-mir-430a-13, dre-mir-430a-14, dre-mir-430a-15, dre-mir-430a-16, dre-mir-430a-17, dre-mir-430a-18, dre-mir-430i-1, dre-mir-430i-3, dre-mir-430b-2, dre-mir-430b-3, dre-mir-430b-4, dre-mir-430b-6, dre-mir-430b-7, dre-mir-430b-8, dre-mir-430b-9, dre-mir-430b-10, dre-mir-430b-11, dre-mir-430b-12, dre-mir-430b-13, dre-mir-430b-14, dre-mir-430b-15, dre-mir-430b-16, dre-mir-430b-17, dre-mir-430b-18, dre-mir-430b-5, dre-mir-430b-19, dre-mir-430b-20, dre-let-7j, dre-mir-181a-2, dre-mir-34b, dre-mir-34c, dre-mir-222b, dre-mir-138-2, dre-mir-181a-4, dre-mir-181a-3, dre-mir-181a-5, dre-mir-181b-3, dre-mir-181d, dre-mir-128-3
For instance, in zebrafish, miR-430 targets a large number of maternally deposited transcripts at the onset of zygotic transcription [6] and many examples of spatially exclusive expression domains of miRNAs and their targets have been documented (for example, [12, 53]). [score:7]
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[+] score: 7
Similar mir-430 clusters are found in other teleost genomes, e. g. on medaka chr-4 and stickleback chromosome-IV. [score:1]
The centromere-proximal portion of the heterochromatic region includes a cluster of 55 members of the miRNA family mir-430 at about 28.0 Mb. [score:1]
Only one mir-430 gene is found outside this cluster, on chr-10 (Table 2, Figure 5A, B, row 2). [score:1]
Chr-4q has accumulated numerous special genetic elements, including 5S RNA motifs, miRNA repeats (especially mir-430 see below), a number of repetitive gene families, snRNA genes, tRNA genes, the transposable element Kolobok-1, and pseudogenes. [score:1]
Mir-430 also regulates the production of Sdf1a, a ligand secreted by somatic tissues that specifies the path along which primary germ cells (PGCs) migrate to the site of the future gonad during gastrulation [109]. [score:1]
One interesting repetitive element on chr-4 is the cluster of 55 members of the mir-430 gene family at about 28.0 Mb. [score:1]
B. Distribution of all repeats and Kolobok elements across chr-4. C. Ideogram showing the location of the miRNA-430 gene cluster, the heterochromatic region of chr-4, and sar4. [score:1]
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MiR-430 specific targeting of tdrd7 may thus explain the decrease in expression levels detected at the 50% epiboly stage (Figure 5A-B). [score:4]
In addition, Tdrd7 has been shown to be a target of miR-430, a miRNA involved in the degradation of zebrafish maternal RNAs pre-MBT [31]. [score:3]
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[+] score: 6
However, the maternally inherited miR-17–92 cluster, which has similar seed sites as miR-430 and human embryonic regulator miRNA (miR-302/miR-467) (44), may be of great importance to developmental biologist in studying their potential roles in degradation of maternal transcripts during early embryogenesis. [score:3]
We were unable to detect pri-miRNA promoters for 55 intergenic pre-miRNAs (excluding the miR-430 cluster) and 42 intronic pre-miRNAs residing in protein coding host genes. [score:1]
Counting the 59 copies of miR-430 in a cluster as a single gene, we annotated pri-miRNA TSS for ∼58% of total pre-miRNAs (Figure 1C). [score:1]
Based on Ensembl annotation (coding and non-coding host genes), overlapping pre-miRNAs were classified as intragenic, and remaining 217 (including 59 copies of miR-430 in cluster) pre-miRNAs as intergenic. [score:1]
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[+] score: 6
In our results, the dre-miR-430a family (including dre-miR-430a, dre-miR-430b, dre-miR-430c and dre-miR-430i) is highly expressed from 512-cell to 6-somite stage, and may be functional in MZT (Figure  5C). [score:3]
For example, zebrafish miR-430 family is one of the most abundant miRNA families at early developmental stages and leads to degradation and clearance of maternal mRNAs for facilitating the maternal-to-zygotic transition (MZT) [18]. [score:2]
For example, as one of the most abundant miRNA families, zebrafish miR-430 family facilitates the MZT by the clearance of maternal mRNAs [18]. [score:1]
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[+] score: 6
A similar expression pattern was observed for miR-430 in zebrafish [18], miR-427 in Xenopus [19] and miR-290 in mouse [21], which are known to play a key role in promoting maternal transcript turnover during maternal-zygotic transition. [score:3]
In particular, zebrafish miR-430 and Xenopus miR-427 (an orthologue of miR-430) are highly expressed at the onset of zygotic transcription and facilitate the deadenylation and clearance of maternal mRNAs during early embryogenesis [18, 19]. [score:3]
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[+] score: 6
The mean deletion frequency was 4.2% for the miR-17-92 cluster and 1.9% for the miR-430 cluster in zebrafish embryonic cells respectively (Fig. 2E and Fig. 2H). [score:1]
For the larger miR-430 cluster deletions, we have outcrossed 32 P0 fish but failed to obtain germline deletions, suggesting that large genomic deletions are not efficiently transmitted. [score:1]
While the miR-17-92 cluster encodes 6 miRNAs, the miR-430 cluster is the largest miRNA cluster in vertebrates encoding 57 miRNAs. [score:1]
Using this TALEN- nanos-3′UTR construct, we have demonstrated that large genomic deletions of the miR-430 cluster could be transmitted through the germline. [score:1]
By screening 23 P0 fish raised from the TALEN- nanos-3′UTR injected embryos, we have obtained 1 founder transmitted the miR-430 deletion to 3/32 embryos (Fig. 4C). [score:1]
To test this dual TALEN strategy we designed two pairs of TALENs to delete the zebrafish miR-17-92 cluster (1.2 kb) and the miR-430 cluster (79.8 kb) (Fig. 2B and Fig. 2F). [score:1]
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In relation, Chen et al. found that in zebrafish embryos, a zebrafish-specific miR-430 family started to express at 4 hpf and this family’s clusters dominated the miRNA profile up to 24 hpf [237]. [score:3]
The miR-430 family was related to human and mouse ES cell-specific miRNAs [239, 240]. [score:1]
At the MBT stage, the majority of maternal mRNAs were degraded with the aid of zygotic miRNAs (miR-430) [201]. [score:1]
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26
[+] score: 5
Other miRNAs from this paper: dre-mir-10a, dre-mir-10b-1, dre-mir-204-1, dre-mir-181a-1, dre-mir-214, dre-mir-222a, dre-mir-430a-1, dre-mir-430b-1, dre-mir-430c-1, dre-let-7a-1, dre-let-7a-2, dre-let-7a-3, dre-let-7a-4, dre-let-7a-5, dre-let-7a-6, dre-let-7b, dre-let-7c-1, dre-let-7c-2, dre-let-7d-1, dre-let-7d-2, dre-let-7e, dre-let-7f, dre-let-7g-1, dre-let-7g-2, dre-let-7h, dre-let-7i, dre-mir-10b-2, dre-mir-10c, dre-mir-10d, dre-mir-17a-1, dre-mir-17a-2, dre-mir-21-1, dre-mir-21-2, dre-mir-22a, dre-mir-22b, dre-mir-25, dre-mir-26a-1, dre-mir-26a-2, dre-mir-26a-3, dre-mir-30d, dre-mir-92a-1, dre-mir-92a-2, dre-mir-92b, dre-mir-100-1, dre-mir-100-2, dre-mir-125a-1, dre-mir-125a-2, dre-mir-125b-1, dre-mir-125b-2, dre-mir-125b-3, dre-mir-125c, dre-mir-126a, dre-mir-143, dre-mir-146a, dre-mir-462, dre-mir-202, dre-mir-204-2, dre-mir-430c-2, dre-mir-430c-3, dre-mir-430c-4, dre-mir-430c-5, dre-mir-430c-6, dre-mir-430c-7, dre-mir-430c-8, dre-mir-430c-9, dre-mir-430c-10, dre-mir-430c-11, dre-mir-430c-12, dre-mir-430c-13, dre-mir-430c-14, dre-mir-430c-15, dre-mir-430c-16, dre-mir-430c-17, dre-mir-430c-18, dre-mir-430a-2, dre-mir-430a-3, dre-mir-430a-4, dre-mir-430a-5, dre-mir-430a-6, dre-mir-430a-7, dre-mir-430a-8, dre-mir-430a-9, dre-mir-430a-10, dre-mir-430a-11, dre-mir-430a-12, dre-mir-430a-13, dre-mir-430a-14, dre-mir-430a-15, dre-mir-430a-16, dre-mir-430a-17, dre-mir-430a-18, dre-mir-430i-1, dre-mir-430i-3, dre-mir-430b-2, dre-mir-430b-3, dre-mir-430b-4, dre-mir-430b-6, dre-mir-430b-7, dre-mir-430b-8, dre-mir-430b-9, dre-mir-430b-10, dre-mir-430b-11, dre-mir-430b-12, dre-mir-430b-13, dre-mir-430b-14, dre-mir-430b-15, dre-mir-430b-16, dre-mir-430b-17, dre-mir-430b-18, dre-mir-430b-5, dre-mir-430b-19, dre-mir-430b-20, dre-let-7j, dre-mir-181a-2, dre-mir-1388, dre-mir-222b, dre-mir-126b, dre-mir-181a-4, dre-mir-181a-3, dre-mir-181a-5, dre-mir-204-3
However, zebrafish miR-430-3p only targets a relatively small number (~10%) of maternal mRNAs 60, and many maternal mRNAs are instead regulated through a maternal decay pathway 61 62. [score:4]
The most studied embryonic miRNA in zebrafish is miR-430-3p, which is transcribed before the maternal to zygotic transition and functions as part of the zygotic decay pathway to destabilize maternal mRNAs 60. [score:1]
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27
[+] score: 4
Other miRNAs from this paper: cel-let-7, cel-mir-1, cel-mir-35, cel-mir-52, cel-mir-58a, dme-mir-1, mmu-let-7g, mmu-let-7i, mmu-mir-1a-1, dme-bantam, mmu-let-7d, dme-let-7, mmu-let-7a-1, mmu-let-7a-2, mmu-let-7b, mmu-let-7c-1, mmu-let-7c-2, mmu-let-7e, mmu-let-7f-1, mmu-let-7f-2, mmu-mir-16-1, mmu-mir-16-2, mmu-mir-1a-2, cel-lsy-6, dre-mir-430a-1, dre-mir-430b-1, dre-mir-430c-1, dre-let-7a-1, dre-let-7a-2, dre-let-7a-3, dre-let-7a-4, dre-let-7a-5, dre-let-7a-6, dre-let-7b, dre-let-7c-1, dre-let-7c-2, dre-let-7d-1, dre-let-7d-2, dre-let-7e, dre-let-7f, dre-let-7g-1, dre-let-7g-2, dre-let-7h, dre-let-7i, dre-mir-1-2, dre-mir-1-1, dre-mir-16a, dre-mir-16b, dre-mir-16c, dre-mir-430c-2, dre-mir-430c-3, dre-mir-430c-4, dre-mir-430c-5, dre-mir-430c-6, dre-mir-430c-7, dre-mir-430c-8, dre-mir-430c-9, dre-mir-430c-10, dre-mir-430c-11, dre-mir-430c-12, dre-mir-430c-13, dre-mir-430c-14, dre-mir-430c-15, dre-mir-430c-16, dre-mir-430c-17, dre-mir-430c-18, dre-mir-430a-2, dre-mir-430a-3, dre-mir-430a-4, dre-mir-430a-5, dre-mir-430a-6, dre-mir-430a-7, dre-mir-430a-8, dre-mir-430a-9, dre-mir-430a-10, dre-mir-430a-11, dre-mir-430a-12, dre-mir-430a-13, dre-mir-430a-14, dre-mir-430a-15, dre-mir-430a-16, dre-mir-430a-17, dre-mir-430a-18, dre-mir-430i-1, dre-mir-430i-3, dre-mir-430b-2, dre-mir-430b-3, dre-mir-430b-4, dre-mir-430b-6, dre-mir-430b-7, dre-mir-430b-8, dre-mir-430b-9, dre-mir-430b-10, dre-mir-430b-11, dre-mir-430b-12, dre-mir-430b-13, dre-mir-430b-14, dre-mir-430b-15, dre-mir-430b-16, dre-mir-430b-17, dre-mir-430b-18, dre-mir-430b-5, dre-mir-430b-19, dre-mir-430b-20, dre-let-7j, mmu-mir-1b, cel-mir-58b, mmu-let-7j, mmu-let-7k, cel-mir-58c
Whereas mutations in the PAM2 motif are sufficient for loss of interaction with PABP, only mutations in both motifs lead to a delay in miR-430-dependant deadenylation (40). [score:3]
Using this strategy, Pabpc1 was depleted in vivo to <5%, yet repression of a miR-430 reporter was unaffected, leading to the suggestion that Pabpc1 is dispensable for silencing in zebrafish embryos (40). [score:1]
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[+] score: 3
In zebrafish, Dnd1 -deficient PGCs show a significant decrease in the expression of exogenously delivered nos1, TDRD7 (Kedde et al., 2007), and hub mRNAs (Mickoleit et al., 2011), which have miR-430 seed sequences located in their 3′-UTRs. [score:3]
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[+] score: 3
Other miRNAs from this paper: dre-mir-7b, dre-mir-7a-1, dre-mir-7a-2, dre-mir-182, dre-mir-183, dre-mir-205, dre-mir-214, dre-mir-430a-1, dre-mir-430b-1, dre-mir-430c-1, dre-let-7a-1, dre-let-7a-2, dre-let-7a-3, dre-let-7a-4, dre-let-7a-5, dre-let-7a-6, dre-let-7b, dre-let-7c-1, dre-let-7c-2, dre-let-7d-1, dre-let-7d-2, dre-let-7e, dre-let-7f, dre-let-7g-1, dre-let-7g-2, dre-let-7h, dre-let-7i, dre-mir-1-2, dre-mir-1-1, dre-mir-7a-3, dre-mir-30c, dre-mir-124-1, dre-mir-124-2, dre-mir-124-3, dre-mir-124-4, dre-mir-124-5, dre-mir-124-6, dre-mir-140, dre-mir-206-1, dre-mir-206-2, dre-mir-375-1, dre-mir-375-2, dre-mir-430c-2, dre-mir-430c-3, dre-mir-430c-4, dre-mir-430c-5, dre-mir-430c-6, dre-mir-430c-7, dre-mir-430c-8, dre-mir-430c-9, dre-mir-430c-10, dre-mir-430c-11, dre-mir-430c-12, dre-mir-430c-13, dre-mir-430c-14, dre-mir-430c-15, dre-mir-430c-16, dre-mir-430c-17, dre-mir-430c-18, dre-mir-430a-2, dre-mir-430a-3, dre-mir-430a-4, dre-mir-430a-5, dre-mir-430a-6, dre-mir-430a-7, dre-mir-430a-8, dre-mir-430a-9, dre-mir-430a-10, dre-mir-430a-11, dre-mir-430a-12, dre-mir-430a-13, dre-mir-430a-14, dre-mir-430a-15, dre-mir-430a-16, dre-mir-430a-17, dre-mir-430a-18, dre-mir-430i-1, dre-mir-430i-3, dre-mir-430b-2, dre-mir-430b-3, dre-mir-430b-4, dre-mir-430b-6, dre-mir-430b-7, dre-mir-430b-8, dre-mir-430b-9, dre-mir-430b-10, dre-mir-430b-11, dre-mir-430b-12, dre-mir-430b-13, dre-mir-430b-14, dre-mir-430b-15, dre-mir-430b-16, dre-mir-430b-17, dre-mir-430b-18, dre-mir-430b-5, dre-mir-430b-19, dre-mir-430b-20, dre-let-7j
Strikingly, the brain phenotype of maternal-zygotic Dicer zebrafish can be restored by injection of miR-430, the most abundant miRNA in early zebrafish development. [score:2]
For example, the role of miR-430 in zebrafish brain morphogenesis has become clear from experiments that rescued Dicer null mutants by injection of an miRNA duplex that mimicked a miR-430 family member [2]. [score:1]
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30
[+] score: 3
Dnd protects critical germ plasm mRNA from degradation in germ cells by inhibiting miR-430 11, 12. [score:3]
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31
[+] score: 3
Other miRNAs from this paper: hsa-let-7a-1, hsa-let-7a-2, hsa-let-7a-3, hsa-let-7b, hsa-let-7c, hsa-let-7d, hsa-let-7e, hsa-let-7f-1, hsa-let-7f-2, hsa-mir-17, hsa-mir-24-1, hsa-mir-24-2, hsa-mir-25, mmu-let-7g, mmu-let-7i, mmu-mir-124-3, mmu-mir-9-2, mmu-mir-134, mmu-mir-137, mmu-mir-138-2, mmu-mir-145a, mmu-mir-24-1, hsa-mir-192, mmu-mir-194-1, mmu-mir-200b, hsa-mir-7-1, hsa-mir-7-2, hsa-mir-7-3, hsa-mir-215, hsa-mir-221, hsa-mir-200b, mmu-mir-296, mmu-let-7d, mmu-mir-106b, hsa-let-7g, hsa-let-7i, hsa-mir-124-1, hsa-mir-124-2, hsa-mir-124-3, hsa-mir-137, hsa-mir-138-2, hsa-mir-145, hsa-mir-9-1, hsa-mir-9-2, hsa-mir-9-3, hsa-mir-134, hsa-mir-138-1, hsa-mir-194-1, mmu-mir-192, mmu-mir-200a, mmu-let-7a-1, mmu-let-7a-2, mmu-let-7b, mmu-let-7c-1, mmu-let-7c-2, mmu-let-7e, mmu-let-7f-1, mmu-let-7f-2, mmu-mir-24-2, mmu-mir-346, hsa-mir-200c, mmu-mir-17, mmu-mir-25, mmu-mir-200c, mmu-mir-221, mmu-mir-124-1, mmu-mir-124-2, mmu-mir-9-1, mmu-mir-9-3, mmu-mir-138-1, mmu-mir-7a-1, mmu-mir-7a-2, mmu-mir-7b, hsa-mir-194-2, mmu-mir-194-2, hsa-mir-106b, hsa-mir-200a, hsa-mir-296, hsa-mir-369, hsa-mir-346, mmu-mir-215, gga-let-7i, gga-let-7a-3, gga-let-7b, gga-let-7c, gga-mir-221, gga-mir-17, gga-mir-138-1, gga-mir-124a, gga-mir-194, gga-mir-215, gga-mir-137, gga-mir-7-2, gga-mir-138-2, gga-let-7g, gga-let-7d, gga-let-7f, gga-let-7a-1, gga-mir-200a, gga-mir-200b, gga-mir-124b, gga-let-7a-2, gga-let-7j, gga-let-7k, gga-mir-7-3, gga-mir-7-1, gga-mir-24, gga-mir-7b, gga-mir-9-2, dre-mir-7b, dre-mir-7a-1, dre-mir-7a-2, dre-mir-192, dre-mir-221, dre-mir-430a-1, dre-mir-430b-1, dre-mir-430c-1, dre-let-7a-1, dre-let-7a-2, dre-let-7a-3, dre-let-7a-4, dre-let-7a-5, dre-let-7a-6, dre-let-7b, dre-let-7c-1, dre-let-7c-2, dre-let-7d-1, dre-let-7d-2, dre-let-7e, dre-let-7f, dre-let-7g-1, dre-let-7g-2, dre-let-7h, dre-let-7i, dre-mir-7a-3, dre-mir-9-1, dre-mir-9-2, dre-mir-9-4, dre-mir-9-3, dre-mir-9-5, dre-mir-9-6, dre-mir-9-7, dre-mir-17a-1, dre-mir-17a-2, dre-mir-24-4, dre-mir-24-2, dre-mir-24-3, dre-mir-24-1, dre-mir-25, dre-mir-92b, dre-mir-124-1, dre-mir-124-2, dre-mir-124-3, dre-mir-124-4, dre-mir-124-5, dre-mir-124-6, dre-mir-137-1, dre-mir-137-2, dre-mir-138-1, dre-mir-145, dre-mir-194a, dre-mir-194b, dre-mir-200a, dre-mir-200b, dre-mir-200c, dre-mir-430c-2, dre-mir-430c-3, dre-mir-430c-4, dre-mir-430c-5, dre-mir-430c-6, dre-mir-430c-7, dre-mir-430c-8, dre-mir-430c-9, dre-mir-430c-10, dre-mir-430c-11, dre-mir-430c-12, dre-mir-430c-13, dre-mir-430c-14, dre-mir-430c-15, dre-mir-430c-16, dre-mir-430c-17, dre-mir-430c-18, dre-mir-430a-2, dre-mir-430a-3, dre-mir-430a-4, dre-mir-430a-5, dre-mir-430a-6, dre-mir-430a-7, dre-mir-430a-8, dre-mir-430a-9, dre-mir-430a-10, dre-mir-430a-11, dre-mir-430a-12, dre-mir-430a-13, dre-mir-430a-14, dre-mir-430a-15, dre-mir-430a-16, dre-mir-430a-17, dre-mir-430a-18, dre-mir-430i-1, dre-mir-430i-3, dre-mir-430b-2, dre-mir-430b-3, dre-mir-430b-4, dre-mir-430b-6, dre-mir-430b-7, dre-mir-430b-8, dre-mir-430b-9, dre-mir-430b-10, dre-mir-430b-11, dre-mir-430b-12, dre-mir-430b-13, dre-mir-430b-14, dre-mir-430b-15, dre-mir-430b-16, dre-mir-430b-17, dre-mir-430b-18, dre-mir-430b-5, dre-mir-430b-19, dre-mir-430b-20, mmu-mir-470, hsa-mir-485, hsa-mir-496, dre-let-7j, mmu-mir-485, mmu-mir-543, mmu-mir-369, hsa-mir-92b, gga-mir-9-1, hsa-mir-671, mmu-mir-671, mmu-mir-496a, mmu-mir-92b, hsa-mir-543, gga-mir-124a-2, mmu-mir-145b, mmu-let-7j, mmu-mir-496b, mmu-let-7k, gga-mir-124c, gga-mir-9-3, gga-mir-145, dre-mir-138-2, dre-mir-24b, gga-mir-9-4, mmu-mir-9b-2, mmu-mir-124b, mmu-mir-9b-1, mmu-mir-9b-3, gga-mir-9b-1, gga-let-7l-1, gga-let-7l-2, gga-mir-9b-2
Direct evidence of the essential role of miRNAs in the CNS development became apparent when abnormal neuronal differentiation and neural tube morphological defects observed in Dicer -deficient zebrafish (Giraldez et al., 2005) were rescued by introduction of miR-430 (Giraldez et al., 2005). [score:3]
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[+] score: 3
Target protectors reveal dampening and balancing of Nodal agonist and antagonist by miR-430. [score:3]
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[+] score: 2
MicroRNA injection can also be used in rescue experiments, for example, in a study on the role of miR-430 on zebrafish brain morphogenesis. [score:1]
Injection of miR-430 in an MZ dicer-mutant zebrafish failed to produce endogenous miR-430 and rescued the mutant phenotype [37]. [score:1]
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[+] score: 2
It has been already shown that the 3′UTR is subject to degradation in somatic cells, but is stabilized in PGCs by interaction with the microRNA, miR-430 [20], [21]. [score:1]
This result clearly shows that the mechanism of nanos1 3′UTR, subject to degradation in somatic cells and stabilization in PGCs by interaction with the microRNA, miR-430 [20], [21], is conserved between these two distantly related fish species, as previously shown among other species [15]. [score:1]
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[+] score: 1
In Zebrafish, miR-430 [20] has been shown to silence maternal RNAs, miR-214 is involved in proper somite specification [21] and miR-375 is necessary for the maintenance of embryonic pancreas integrity [22]. [score:1]
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36
[+] score: 1
It contains an accumulation of 5S rRNA, snRNA, tRNA and mir-430 clusters [42, 43], as well as the expanded protein coding gene families described here. [score:1]
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[+] score: 1
Thatcher et al. [25] also confirmed the presence of miR-430 family in two large clusters of 10 and 57 genes on chromosome 4. The reads which did not show any hits in miRBase were analyzed by BLAST against the Rfam database (ftp. [score:1]
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[+] score: 1
The size of the Nodal signaling domain is determined by the interplay between Ndr1/2, the Nodal antagonists Lefty1 and Lefty2 (Lft1 and Lft2, respectively) and the miR-430 family of microRNAs (van Boxtel et al., 2015). [score:1]
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