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20 publications mentioning zma-MIR162

Open access articles that are associated with the species Zea mays and mention the gene name MIR162. Click the [+] symbols to view sentences that include the gene name, or the word cloud on the right for a summary.

1
[+] score: 24
Other miRNAs from this paper: hsa-mir-604
Maize event MIR162 expresses a Vip3Aa20 protein for control of lepidopteran insect pests and a phosphomannose isomerase (PMI) protein that was used as a selectable marker. [score:3]
As expected, PMI concentrations in Bt11 × MIR162 × MIR604 × GA21 maize grain were higher than those in MIR162 or MIR604 due to the expression of two pmi genes, each contributed by MIR162 and MIR604 (Figure  5). [score:3]
The concentrations of Cry1Ab in Bt11 × MIR162 × GA21 and Vip3Aa20 in Bt11 × MIR162× MIR604 × GA21 were generally higher than in the other stacks (Figures  1 and 4) but were still within the ranges of expression for the single events (Bt11 and MIR162) grown in the same trials and in the same germplasm. [score:3]
Compositional equivalence, protein expression and molecular characterization (insert stability and intactness) data generated for food and feed safety assessments of stacked GM events and the component single events are described for stacks containing two, three and four Syngenta events in six stacked event combinations (Bt11 × GA21, Bt11 × MIR604, MIR604 × GA21, Bt11 × MIR604 × GA21, Bt11 × MIR162 × GA21 and Bt11 × MIR604 × MIR162 × GA21). [score:1]
N =  170 (Bt11), 32 (Bt11 ×  GA21), 5 (Bt11 ×  MIR604), 29 (Bt11 ×  MIR604 ×  GA21), 10 (Bt11 ×  MIR162 ×  GA21) and 9 (Bt11 ×  MIR162 ×  MIR604 ×  GA21); Number of environments (combination of germplasm, year grown and location) = 11 (Bt11), 4 (Bt11 ×  GA21), 1 (Bt11 ×  MIR604), 3 (Bt11 ×  MIR604 ×  GA21), 1 (Bt11 ×  MIR162 ×  GA21), 1 (Bt11 ×  MIR162 ×  MIR604 ×  GA21); × = individual datapoints for Cry1Ab in event Bt11 and Bt11 ×  MIR162 ×  GA21 grown together in one field trial. [score:1]
Data from studies conducted over a 15‐year period for several stacked GM event maize (Zea mays L. ) products (Bt11 ×  GA21, Bt11 ×  MIR604, MIR604 ×  GA21, Bt11 ×  MIR604 ×  GA21, Bt11 ×  MIR162 ×  GA21 and Bt11 ×  MIR604 ×  MIR162 ×  GA21), together with their component single events, are presented. [score:1]
For analysis of Bt11, MIR162 and MIR604 inserts, plasmid DNA representing one copy per maize genome, based on plasmid size, was included to serve as a positive control for each of the genes of interest. [score:1]
The Southern analyses had the expected hybridization patterns of the cry1Ab and pat genes from Bt11, the vip3Aa20 and pmi genes from MIR162, the mcry3A and pmi genes from MIR604 and the mepsps gene from GA21 contained in the stacks. [score:1]
An example Southern blot analysis for pmi is shown in Figure  6. These results demonstrated that inheritance and integrity of the transgenic inserts were preserved during conventional breeding for production of the stacks Bt11 × GA21, Bt11 × MIR604, MIR604 × GA21, Bt11 × MIR604 × GA21, Bt11 × MIR162× GA21 and Bt11 × MIR162 × MIR604 × GA21 maize. [score:1]
The events and stacked GM events described herein consisted of the maize events Bt11, MIR162, MIR604 and GA21, and several stacks created by combining these events through conventional breeding. [score:1]
Figure 4Vip3Aa20 concentrations in event MIR162 (dark grey) and stacks containing event MIR162. [score:1]
Stacked GM events were produced by combining the individual events Bt11, MIR162, MIR604 and GA21 through conventional breeding. [score:1]
N =  52 (MIR162), 10 (Bt11 ×  MIR162 ×  GA21) and 9 (Bt11 ×  MIR162 ×  MIR604 ×  GA21); Number of environments (combination of germplasm, year grown and location) = 6 (MIR162), 1 (Bt11 ×  MIR162 ×  GA21), 1 (Bt11 ×  MIR162 ×  MIR604 ×  GA21); × = individual datapoints for Vip3Aa20 in MIR162 maize and Bt11 ×  MIR162 ×  MIR604 ×  GA21 maize grown together in one field trial. [score:1]
Figure 6Southern blot analysis confirming presence of pmi in event MIR162, event MIR604 and in Bt11 ×  MIR162 ×  MIR604 ×  GA21. [score:1]
Figure 5Phosphomannose isomerase concentrations in event MIR604, event MIR162 (both dark grey) and stacks containing events MIR604 and MIR162. [score:1]
N =  101 (MIR604), 52 (MIR162), 5 (MIR604 ×  GA21), 5 (Bt11 ×  MIR604), 29 (Bt11 ×  MIR604 ×  GA21), 10 (Bt11 ×  MIR162 ×  GA21) and 17 (Bt11 ×  MIR162 ×  MIR604 ×  GA21); Number of environments (combination of germplasm, year grown and location) = 7 (MIR604), 6 (MIR162), 1 (MIR604 ×  GA21), 1 (Bt11 ×  MIR604), 3 (Bt11 ×  MIR604 ×  GA21), 1 (Bt11 ×  MIR162 ×  GA21) and 1 (Bt11 ×  MIR162 ×  MIR604 ×  GA21). [score:1]
N =  208 (GA21), 5 (MIR604 ×  GA21), 30 (Bt11 ×  GA21), 29 (Bt11 ×  MIR604 ×  GA21), 10 (Bt11 ×  MIR162 ×  GA21) and 8 (Bt11 ×  MIR162 ×  MIR604 ×  GA21); Number of environments (combination of germplasm, year grown and location) = 12 (GA21), 1 (MIR604 ×  GA21), 4 (Bt11 ×  GA21), 3 (Bt11 ×  MIR604 ×  GA21), 1 (Bt11 ×  MIR162 ×  GA21) and 1 (Bt11 ×  MIR162 ×  MIR604 ×  GA21). [score:1]
N =  101 (MIR604), 5 (MIR604 ×  GA21), 5 (Bt11 ×  MIR604), 19 (Bt11 ×  MIR604 ×  GA21) and 8 (Bt11 ×  MIR162 ×  MIR604 ×  GA21); Number of environments (combination of germplasm, year grown and location) = 7 (MIR604), 1 (MIR604 ×  GA21), 1 (Bt11 ×  MIR604), 3 (Bt11 ×  MIR604 ×  GA21) and 1 (Bt11 ×  MIR162 ×  MIR604 ×  GA21). [score:1]
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2
[+] score: 17
In shoots, the expression of MIR162, MIR167s, and MIR394s was upregulated by N deficiency, whereas the expression of MIR169s, MIR397s, MIR398s, MIR408s, and MIR528s was significantly downregulated by N deficiency (Figure 3A). [score:11]
MIR162 and MIR167s were also upregulated by N deficiency in roots, and the expressions of MIR169s, MIR397s, MIR408s, MIR528s, MIR395s, and MIR827 were clearly decreased in N -deficient roots (Figure 3B). [score:6]
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3
[+] score: 16
On average, excluding the target at 40 or 20 copies, the mixtures contained the following concentration of target copies per reaction: 56378 of hmgA, 186 of MON863, 136 of MON810, 278 of DP98140, 9735 of MON89034, 1715 of MIR604, 18537 of MIR162 and 1573 of GA21. [score:5]
To show that the tetraplex assays can be combined for multiplex quantification of different targets, two assays were developed, named MTQ1 (targeting GM maize events MON810, DP98140, MON863 and hmgA maize endogene) and MTQ2 (targeting GM maize events MIR162, MIR604, MON89034 and GA21). [score:5]
Linear response in the quantitative range was observed for all the tested targets (R [2] for hmgA, MON863, MON810, DP98140, MIR604, GA21, MON89034 and MIR162 were 0.9885, 0.9920, 0.9922, 0.9913, 0.9954, 0.9988, 0.9901 and 0.9986, respectively). [score:3]
In MTQ2 the aLOD was determined to be at 9 copies for GA21, 11 copies for MIR162, 10 copies for MIR604 and 11 copies for MON89034. [score:1]
In MTQ2 the aLOQ was determined to be at least 31 copies for GA21, 41 copies for MIR162, 36 copies for MIR604 and 42 copies for MON89034 (Table 2). [score:1]
In MTQ2 the aLOQ was determined to be at least 11 copies for GA21, 49 copies for MIR162, 13 copies for MIR604 and 13 copies for MON89034 (Supplementary Table S3). [score:1]
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4
[+] score: 10
Other miRNAs from this paper: zma-MIR156d, zma-MIR156f, zma-MIR156g, zma-MIR156b, zma-MIR156c, zma-MIR156e, zma-MIR156a, zma-MIR156h, zma-MIR156i, zma-MIR160a, zma-MIR160c, zma-MIR160d, zma-MIR160b, zma-MIR164a, zma-MIR164d, zma-MIR164b, zma-MIR164c, zma-MIR169a, zma-MIR169b, zma-MIR167a, zma-MIR167b, zma-MIR167d, zma-MIR167c, zma-MIR160e, zma-MIR166a, zma-MIR166h, zma-MIR166e, zma-MIR166i, zma-MIR166f, zma-MIR166g, zma-MIR166b, zma-MIR166c, zma-MIR166d, zma-MIR171a, zma-MIR171b, zma-MIR172a, zma-MIR172d, zma-MIR172b, zma-MIR172c, zma-MIR171d, zma-MIR171f, zma-MIR394a, zma-MIR394b, zma-MIR395b, zma-MIR395c, zma-MIR395a, zma-MIR396b, zma-MIR396a, zma-MIR399a, zma-MIR399c, zma-MIR399b, zma-MIR399d, zma-MIR399e, zma-MIR399f, zma-MIR156j, zma-MIR159a, zma-MIR159b, zma-MIR159c, zma-MIR159d, zma-MIR319a, zma-MIR319c, zma-MIR319b, zma-MIR319d, zma-MIR166k, zma-MIR166j, zma-MIR167e, zma-MIR167f, zma-MIR167g, zma-MIR167h, zma-MIR167i, zma-MIR168a, zma-MIR168b, zma-MIR169c, zma-MIR169f, zma-MIR169g, zma-MIR169h, zma-MIR169i, zma-MIR169k, zma-MIR169j, zma-MIR169d, zma-MIR169e, zma-MIR171c, zma-MIR171j, zma-MIR171e, zma-MIR171i, zma-MIR171g, zma-MIR172e, zma-MIR166l, zma-MIR166m, zma-MIR171k, zma-MIR171h, zma-MIR393a, zma-MIR408a, zma-MIR156k, zma-MIR160f, zma-MIR396c, zma-MIR396d, zma-MIR156l, zma-MIR159e, zma-MIR159f, zma-MIR159g, zma-MIR159h, zma-MIR159i, zma-MIR159j, zma-MIR159k, zma-MIR160g, zma-MIR164e, zma-MIR164f, zma-MIR164g, zma-MIR164h, zma-MIR166n, zma-MIR167j, zma-MIR169l, zma-MIR169m, zma-MIR169n, zma-MIR169o, zma-MIR169p, zma-MIR169q, zma-MIR169r, zma-MIR171l, zma-MIR171m, zma-MIR171n, zma-MIR393b, zma-MIR393c, zma-MIR395d, zma-MIR395e, zma-MIR395f, zma-MIR395g, zma-MIR395h, zma-MIR395i, zma-MIR395j, zma-MIR395k, zma-MIR395l, zma-MIR395m, zma-MIR395n, zma-MIR395o, zma-MIR395p, zma-MIR396e, zma-MIR396f, zma-MIR396g, zma-MIR396h, zma-MIR397a, zma-MIR397b, zma-MIR398a, zma-MIR398b, zma-MIR399g, zma-MIR399h, zma-MIR399i, zma-MIR399j, zma-MIR408b, zma-MIR482, zma-MIR528a, zma-MIR528b, zma-MIR529, zma-MIR827, zma-MIR1432, zma-MIR444a, zma-MIR444b
We noted that miR482 lacks an expression signature in any of the five tissues, while miR162 miR394, miR395, miR398, miR399, miR408, miR528 and miR1432 had low expression counts (less than 200 RPM). [score:5]
For example, miR162 targets DCL1 [90], but this target (GRMZM2G040762 in maize) was excluded by our pipeline due to the presence of a 1nt bulge in the alignment with miR162. [score:5]
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5
[+] score: 7
For e. g. Attribute (expressing Cry1Ab toxin, event Bt11) and Attribute II (expressing Cry1Ab and Vip3A, event MIR162) hybrids from Syngenta Seeds, and Performance Series (PS) hybrids (expressing the Cry1A. [score:7]
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[+] score: 7
Other miRNAs from this paper: ghr-MIR162a
For sweet corn production, Attribute (expressing Cry1Ab toxin, event Bt11) and Attribute II (expressing Cry1Ab and Vip3A, event MIR162) hybrids from Syngenta Seeds, and Performance Series (PS) hybrids (expressing the Cry1A. [score:7]
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7
[+] score: 7
For example, miR162 was not found in our research; miR156, miR164, miR167, and miR396 were not down-regulated in the roots, but in the leaves, miR164 showed a down-regulation. [score:7]
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8
[+] score: 7
Other miRNAs from this paper: ath-MIR156a, ath-MIR156b, ath-MIR156c, ath-MIR156d, ath-MIR156e, ath-MIR156f, ath-MIR159a, ath-MIR160a, ath-MIR160b, ath-MIR160c, ath-MIR162a, ath-MIR162b, ath-MIR164a, ath-MIR164b, ath-MIR166a, ath-MIR166b, ath-MIR166c, ath-MIR166d, ath-MIR166e, ath-MIR166f, ath-MIR166g, ath-MIR167a, ath-MIR167b, ath-MIR168a, ath-MIR168b, ath-MIR169a, ath-MIR172a, ath-MIR172b, ath-MIR159b, osa-MIR156a, osa-MIR156b, osa-MIR156c, osa-MIR156d, osa-MIR156e, osa-MIR156f, osa-MIR156g, osa-MIR156h, osa-MIR156i, osa-MIR156j, osa-MIR160a, osa-MIR160b, osa-MIR160c, osa-MIR160d, osa-MIR162a, osa-MIR164a, osa-MIR164b, osa-MIR166a, osa-MIR166b, osa-MIR166c, osa-MIR166d, osa-MIR166e, osa-MIR166f, osa-MIR167a, osa-MIR167b, osa-MIR167c, osa-MIR169a, ath-MIR167d, ath-MIR169b, ath-MIR169c, ath-MIR169d, ath-MIR169e, ath-MIR169f, ath-MIR169g, ath-MIR169h, ath-MIR169i, ath-MIR169j, ath-MIR169k, ath-MIR169l, ath-MIR169m, ath-MIR169n, ath-MIR172c, ath-MIR172d, ath-MIR395a, ath-MIR395b, ath-MIR395c, ath-MIR395d, ath-MIR395e, ath-MIR395f, ath-MIR396a, ath-MIR396b, ath-MIR399a, ath-MIR399b, ath-MIR399c, ath-MIR399d, ath-MIR399e, ath-MIR399f, osa-MIR395b, osa-MIR395d, osa-MIR395e, osa-MIR395g, osa-MIR395h, osa-MIR395i, osa-MIR395j, osa-MIR395k, osa-MIR395l, osa-MIR395s, osa-MIR395t, osa-MIR395c, osa-MIR395a, osa-MIR395f, osa-MIR395u, osa-MIR396a, osa-MIR396b, osa-MIR396c, osa-MIR399a, osa-MIR399b, osa-MIR399c, osa-MIR399d, osa-MIR399e, osa-MIR399f, osa-MIR399g, osa-MIR399h, osa-MIR399i, osa-MIR399j, osa-MIR399k, ath-MIR408, ath-MIR156g, ath-MIR156h, ath-MIR159c, ath-MIR164c, ath-MIR167c, ath-MIR172e, osa-MIR156k, osa-MIR156l, osa-MIR159a, osa-MIR159b, osa-MIR159c, osa-MIR159d, osa-MIR159e, osa-MIR159f, osa-MIR160e, osa-MIR160f, osa-MIR162b, osa-MIR164c, osa-MIR164d, osa-MIR164e, osa-MIR166k, osa-MIR166l, osa-MIR167d, osa-MIR167e, osa-MIR167f, osa-MIR167g, osa-MIR167h, osa-MIR167i, osa-MIR168a, osa-MIR168b, osa-MIR169b, osa-MIR169c, osa-MIR169d, osa-MIR169e, osa-MIR169f, osa-MIR169g, osa-MIR169h, osa-MIR169i, osa-MIR169j, osa-MIR169k, osa-MIR169l, osa-MIR169m, osa-MIR169n, osa-MIR169o, osa-MIR169p, osa-MIR169q, osa-MIR172a, osa-MIR172b, osa-MIR172c, osa-MIR166g, osa-MIR166h, osa-MIR166i, osa-MIR171h, osa-MIR408, osa-MIR172d, osa-MIR167j, osa-MIR166m, osa-MIR166j, osa-MIR164f, zma-MIR156d, zma-MIR156f, zma-MIR156g, zma-MIR156b, zma-MIR156c, zma-MIR156e, zma-MIR156a, zma-MIR156h, zma-MIR156i, zma-MIR160a, zma-MIR160c, zma-MIR160d, zma-MIR160b, zma-MIR164a, zma-MIR164d, zma-MIR164b, zma-MIR164c, zma-MIR169a, zma-MIR169b, zma-MIR167a, zma-MIR167b, zma-MIR167d, zma-MIR167c, zma-MIR160e, zma-MIR166a, zma-MIR166h, zma-MIR166e, zma-MIR166i, zma-MIR166f, zma-MIR166g, zma-MIR166b, zma-MIR166c, zma-MIR166d, zma-MIR172a, zma-MIR172d, zma-MIR172b, zma-MIR172c, osa-MIR396e, zma-MIR395b, zma-MIR395c, zma-MIR395a, zma-MIR396b, zma-MIR396a, zma-MIR399a, zma-MIR399c, zma-MIR399b, zma-MIR399d, zma-MIR399e, zma-MIR399f, zma-MIR156j, zma-MIR159a, zma-MIR159b, zma-MIR159c, zma-MIR159d, zma-MIR166k, zma-MIR166j, zma-MIR167e, zma-MIR167f, zma-MIR167g, zma-MIR167h, zma-MIR167i, zma-MIR168a, zma-MIR168b, zma-MIR169c, zma-MIR169f, zma-MIR169g, zma-MIR169h, zma-MIR169i, zma-MIR169k, zma-MIR169j, zma-MIR169d, zma-MIR169e, zma-MIR172e, zma-MIR166l, zma-MIR166m, zma-MIR171h, zma-MIR408a, zma-MIR156k, zma-MIR160f, osa-MIR529a, osa-MIR395m, osa-MIR395n, osa-MIR395o, osa-MIR395p, osa-MIR395q, osa-MIR395v, osa-MIR395w, osa-MIR395r, osa-MIR529b, osa-MIR169r, osa-MIR396f, zma-MIR396c, zma-MIR396d, osa-MIR2118a, osa-MIR2118b, osa-MIR2118c, osa-MIR2118d, osa-MIR2118e, osa-MIR2118f, osa-MIR2118g, osa-MIR2118h, osa-MIR2118i, osa-MIR2118j, osa-MIR2118k, osa-MIR2118l, osa-MIR2118m, osa-MIR2118n, osa-MIR2118o, osa-MIR2118p, osa-MIR2118q, osa-MIR2118r, osa-MIR2275a, osa-MIR2275b, zma-MIR2118a, zma-MIR2118b, zma-MIR2118c, zma-MIR2118d, zma-MIR2118e, zma-MIR2118f, zma-MIR2118g, zma-MIR2275a, zma-MIR2275b, zma-MIR2275c, zma-MIR2275d, osa-MIR396g, osa-MIR396h, osa-MIR396d, zma-MIR156l, zma-MIR159e, zma-MIR159f, zma-MIR159g, zma-MIR159h, zma-MIR159i, zma-MIR159j, zma-MIR159k, zma-MIR160g, zma-MIR164e, zma-MIR164f, zma-MIR164g, zma-MIR164h, zma-MIR166n, zma-MIR167j, zma-MIR169l, zma-MIR169m, zma-MIR169n, zma-MIR169o, zma-MIR169p, zma-MIR169q, zma-MIR169r, zma-MIR395d, zma-MIR395e, zma-MIR395f, zma-MIR395g, zma-MIR395h, zma-MIR395i, zma-MIR395j, zma-MIR395k, zma-MIR395l, zma-MIR395m, zma-MIR395n, zma-MIR395o, zma-MIR395p, zma-MIR396e, zma-MIR396f, zma-MIR396g, zma-MIR396h, zma-MIR399g, zma-MIR399h, zma-MIR399i, zma-MIR399j, zma-MIR408b, zma-MIR529, osa-MIR395x, osa-MIR395y, osa-MIR2275c, osa-MIR2275d, ath-MIR156i, ath-MIR156j
In addition to the miRNAs mentioned above, one miRNA family (miR162) targets a gene central to miRNA genesis; the differentially expressed zma-miR162 targets DICER-LIKE1 (DCL1), a homolog of DCL1 in Arabidopsis that is required for miRNA accumulation [60]. [score:7]
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9
[+] score: 5
A commitment was made to plant the refuge, and it was decided that Bt materials expressing different modes of action (e. g., Cry1Ab or Vip3A protein as in event MIR162) would be used exclusively (as they had not been affected by the resistant biotype). [score:3]
[Leptra is the marketing name for the breeding stack of maize events TC1507 × MON810 × MIR162 × NK603. [score:1]
Viptera3 here refers to the breeding stack of maize events Bt11 × GA21 × MIR162. [score:1]
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[+] score: 4
The detected miRNAs in these samples have a significant variety in relative expression abundance, with over 70,000 RPM of miR168 and 1 RPM of miR162 in the 28 DAP sample. [score:3]
0125800.g002 Fig 2 Six novel maize miRNAs (miRt4, miRt13, miRt15, miRt17 and miRt21, miRt28) and six conserved miRNAs (miR156, miR162, miR172, miR393, miR396 and miR408) were chosen to verify the sequencing results via the qRT-PCR analysis. [score:1]
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11
[+] score: 4
Finally, the Bahia-Bt strain showed 100% mortality in maize hybrids producing Vip3Aa20 (MIR162 and Bt11/MIR162), indicating that selection for resistance to Cry1A. [score:1]
105 + Cry2Ab, event MON89034 (Monsanto DKB390PRO2); Vip, event MIR162 (Syngenta Status Viptera); Vip + Cry1Ab, events MIR162 + Bt11 (Syngenta Status TL Viptera). [score:1]
The other three were pyramided Bt maize hybrids with the following events: MON89034 (DKB390PRO2, Monsanto do Brasil, São Paulo, SP), Bt11/MIR162 (Status Viptera, Syngenta, São Paulo, SP, Brazil), and TC1507/MON810 (30F53YH; Dupont Pioneer, Santa do Cruz do Sul, RS, Brazil), producing respectively the Bt proteins Cry1A. [score:1]
Three of them were single-protein Bt events: TC1507 (30F53H; Dupont Pioneer, Santa do Cruz do Sul, RS, Brazil), MON810 (30F53Y; Dupont Pioneer, Santa do Cruz do Sul, RS, Brazil), and MIR162 (Agrisure Viptera; Syngenta, São Paulo, SP, Brazil), which respectively produce Cry1Fa, Cry1Ab, and Vip3Aa20. [score:1]
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[+] score: 3
In the identified miRNA families (Fig 2), miR166 consisted of 14 members, miR156 and miR167 had 12 and 10 members, respectively, and miR162, miR529, miR827 and miR1432 had only one member. [score:1]
0164026.g002 Fig 2 miR166 consisted of 14 members, miR156 and miR167 had 12 and 10 members, respectively, and miR162, miR529, miR827 and miR1432 had only one member. [score:1]
miR166 consisted of 14 members, miR156 and miR167 had 12 and 10 members, respectively, and miR162, miR529, miR827 and miR1432 had only one member. [score:1]
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[+] score: 3
In particular, miR156 miR162 exhibited pronounced changes in expression under severe drought stress, suggesting that they may contribute to the acclimatization process [15]. [score:3]
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14
[+] score: 2
Methods of transformation Name of deregulated commercial event 1. Chemically mediated introduction into protoplasts and regeneration T14, T25 2. Electroporation MS3, MS6 3. Microparticle bombardment of plant cells or tissue 676, 678, 680, Bt11 (X4334CBR, X4734CBR), Bt176 (176), CBH-351, DBT418, DLL25 (B16), GA21, LY038, MON801 (MON80100), MON802, MON809, MON810, MON832, MON863, NK603, TC1507 4. Whiskers -mediated plant transformation DAS40278 5. Agrobacterium tumefaciens -mediated plant transformation 32138, 3272, 33121, 4114, 5307, 59122, 98140, Bt10, MIR162, MIR604, MON87411, MON87427, MON87460, MON88017, MON89034, TC6275, VCO-Ø1981-5 6. Aerosol Beam Injection HCEM485 Source: ISAAA GM Crops Approval Database. [score:2]
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[+] score: 2
Other miRNAs from this paper: zma-MIR156d, zma-MIR156f, zma-MIR156g, zma-MIR156b, zma-MIR156c, zma-MIR156e, zma-MIR156a, zma-MIR156h, zma-MIR156i, zma-MIR160a, zma-MIR160c, zma-MIR160d, zma-MIR160b, zma-MIR164a, zma-MIR164d, zma-MIR164b, zma-MIR164c, zma-MIR169a, zma-MIR169b, zma-MIR167a, zma-MIR167b, zma-MIR167d, zma-MIR167c, zma-MIR160e, zma-MIR166a, zma-MIR166h, zma-MIR166e, zma-MIR166i, zma-MIR166f, zma-MIR166g, zma-MIR166b, zma-MIR166c, zma-MIR166d, zma-MIR172a, zma-MIR172d, zma-MIR172b, zma-MIR172c, zma-MIR394a, zma-MIR394b, zma-MIR395b, zma-MIR395c, zma-MIR395a, zma-MIR399a, zma-MIR399c, zma-MIR399b, zma-MIR399d, zma-MIR399e, zma-MIR399f, zma-MIR156j, zma-MIR159a, zma-MIR159b, zma-MIR159c, zma-MIR159d, zma-MIR319a, zma-MIR319c, zma-MIR319b, zma-MIR319d, zma-MIR166k, zma-MIR166j, zma-MIR167e, zma-MIR167f, zma-MIR167g, zma-MIR167h, zma-MIR167i, zma-MIR168a, zma-MIR168b, zma-MIR169c, zma-MIR169f, zma-MIR169g, zma-MIR169h, zma-MIR169i, zma-MIR169k, zma-MIR169j, zma-MIR169d, zma-MIR169e, zma-MIR172e, zma-MIR166l, zma-MIR166m, zma-MIR393a, zma-MIR408a, zma-MIR156k, zma-MIR160f, zma-MIR2118a, zma-MIR2118b, zma-MIR2118c, zma-MIR2118d, zma-MIR2118e, zma-MIR2118f, zma-MIR2118g, zma-MIR2275a, zma-MIR2275b, zma-MIR2275c, zma-MIR2275d, zma-MIR156l, zma-MIR159e, zma-MIR159f, zma-MIR159g, zma-MIR159h, zma-MIR159i, zma-MIR159j, zma-MIR159k, zma-MIR160g, zma-MIR164e, zma-MIR164f, zma-MIR164g, zma-MIR164h, zma-MIR166n, zma-MIR167j, zma-MIR169l, zma-MIR169m, zma-MIR169n, zma-MIR169o, zma-MIR169p, zma-MIR169q, zma-MIR169r, zma-MIR393b, zma-MIR393c, zma-MIR395d, zma-MIR395e, zma-MIR395f, zma-MIR395g, zma-MIR395h, zma-MIR395i, zma-MIR395j, zma-MIR395k, zma-MIR395l, zma-MIR395m, zma-MIR395n, zma-MIR395o, zma-MIR395p, zma-MIR397a, zma-MIR397b, zma-MIR398a, zma-MIR398b, zma-MIR399g, zma-MIR399h, zma-MIR399i, zma-MIR399j, zma-MIR408b, zma-MIR482, zma-MIR528a, zma-MIR528b, zma-MIR529, zma-MIR827, zma-MIR1432, zma-MIR444a, zma-MIR444b
The largest miRNA family size identified was miR166 that consisted of 14 members and miR156/157, miR167 and miR169 possessed 12, 10 and 9 members, respectively; whereas miR162, miR529, miR827 and miR1432 had only one member detected in the maize seeds (Figure 4). [score:1]
The most abundant families were miR156/miR157, miR166, miR168 and miR528, and the least abundant families were miR162 and miR399 (Figure 3). [score:1]
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[+] score: 1
PMI enabled Syngenta to successfully develop several commercialized events including enhanced starch processing trait Enogen™ (Event 3272) and insect resistant trait events including Agrisure® RW (Event MIR604), Agrisure Viptera® (Event MIR162), and Agrisure Duracade™ (Event 5307) (Table 1). [score:1]
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[+] score: 1
Other miRNAs from this paper: ath-MIR156a, ath-MIR156b, ath-MIR156c, ath-MIR156d, ath-MIR156e, ath-MIR156f, ath-MIR159a, ath-MIR160a, ath-MIR160b, ath-MIR160c, ath-MIR162a, ath-MIR162b, ath-MIR164a, ath-MIR164b, ath-MIR166a, ath-MIR166b, ath-MIR166c, ath-MIR166d, ath-MIR166e, ath-MIR166f, ath-MIR166g, ath-MIR167a, ath-MIR167b, ath-MIR169a, ath-MIR171a, ath-MIR172a, ath-MIR172b, ath-MIR159b, osa-MIR156a, osa-MIR156b, osa-MIR156c, osa-MIR156d, osa-MIR156e, osa-MIR156f, osa-MIR156g, osa-MIR156h, osa-MIR156i, osa-MIR156j, osa-MIR160a, osa-MIR160b, osa-MIR160c, osa-MIR160d, osa-MIR162a, osa-MIR164a, osa-MIR164b, osa-MIR166a, osa-MIR166b, osa-MIR166c, osa-MIR166d, osa-MIR166e, osa-MIR166f, osa-MIR167a, osa-MIR167b, osa-MIR167c, osa-MIR169a, osa-MIR171a, ath-MIR167d, ath-MIR169b, ath-MIR169c, ath-MIR169d, ath-MIR169e, ath-MIR169f, ath-MIR169g, ath-MIR169h, ath-MIR169i, ath-MIR169j, ath-MIR169k, ath-MIR169l, ath-MIR169m, ath-MIR169n, ath-MIR171b, ath-MIR171c, ath-MIR172c, ath-MIR172d, ath-MIR393a, ath-MIR393b, ath-MIR394a, ath-MIR394b, ath-MIR395a, ath-MIR395b, ath-MIR395c, ath-MIR395d, ath-MIR395e, ath-MIR395f, osa-MIR393a, osa-MIR394, osa-MIR395b, osa-MIR395d, osa-MIR395e, osa-MIR395g, osa-MIR395h, osa-MIR395i, osa-MIR395j, osa-MIR395k, osa-MIR395l, osa-MIR395s, osa-MIR395t, osa-MIR395c, osa-MIR395a, osa-MIR395f, osa-MIR395u, ath-MIR156g, ath-MIR156h, ath-MIR159c, ath-MIR164c, ath-MIR167c, ath-MIR172e, osa-MIR156k, osa-MIR156l, osa-MIR159a, osa-MIR159b, osa-MIR159c, osa-MIR159d, osa-MIR159e, osa-MIR159f, osa-MIR160e, osa-MIR160f, osa-MIR162b, osa-MIR164c, osa-MIR164d, osa-MIR164e, osa-MIR166k, osa-MIR166l, osa-MIR167d, osa-MIR167e, osa-MIR167f, osa-MIR167g, osa-MIR167h, osa-MIR167i, osa-MIR169b, osa-MIR169c, osa-MIR169d, osa-MIR169e, osa-MIR169f, osa-MIR169g, osa-MIR169h, osa-MIR169i, osa-MIR169j, osa-MIR169k, osa-MIR169l, osa-MIR169m, osa-MIR169n, osa-MIR169o, osa-MIR169p, osa-MIR169q, osa-MIR171b, osa-MIR171c, osa-MIR171d, osa-MIR171e, osa-MIR171f, osa-MIR171g, osa-MIR172a, osa-MIR172b, osa-MIR172c, osa-MIR166g, osa-MIR166h, osa-MIR166i, osa-MIR171h, osa-MIR393b, osa-MIR172d, osa-MIR171i, osa-MIR167j, osa-MIR166m, osa-MIR166j, osa-MIR164f, zma-MIR156d, zma-MIR156f, zma-MIR156g, zma-MIR156b, zma-MIR156c, zma-MIR156e, zma-MIR156a, zma-MIR156h, zma-MIR156i, zma-MIR160a, zma-MIR160c, zma-MIR160d, zma-MIR160b, zma-MIR164a, zma-MIR164d, zma-MIR164b, zma-MIR164c, zma-MIR169a, zma-MIR169b, zma-MIR167a, zma-MIR167b, zma-MIR167d, zma-MIR167c, zma-MIR160e, zma-MIR166a, zma-MIR166h, zma-MIR166e, zma-MIR166i, zma-MIR166f, zma-MIR166g, zma-MIR166b, zma-MIR166c, zma-MIR166d, zma-MIR171a, zma-MIR171b, zma-MIR172a, zma-MIR172d, zma-MIR172b, zma-MIR172c, zma-MIR171d, zma-MIR171f, zma-MIR394a, zma-MIR394b, zma-MIR395b, zma-MIR395c, zma-MIR395a, zma-MIR156j, zma-MIR159a, zma-MIR159b, zma-MIR159c, zma-MIR159d, zma-MIR166k, zma-MIR166j, zma-MIR167e, zma-MIR167f, zma-MIR167g, zma-MIR167h, zma-MIR167i, zma-MIR169c, zma-MIR169f, zma-MIR169g, zma-MIR169h, zma-MIR169i, zma-MIR169k, zma-MIR169j, zma-MIR169d, zma-MIR169e, zma-MIR171c, zma-MIR171j, zma-MIR171e, zma-MIR171i, zma-MIR171g, zma-MIR172e, zma-MIR166l, zma-MIR166m, zma-MIR171k, zma-MIR171h, zma-MIR393a, zma-MIR156k, zma-MIR160f, osa-MIR528, osa-MIR529a, osa-MIR395m, osa-MIR395n, osa-MIR395o, osa-MIR395p, osa-MIR395q, osa-MIR395v, osa-MIR395w, osa-MIR395r, ath-MIR827, osa-MIR529b, osa-MIR1432, osa-MIR169r, osa-MIR827, osa-MIR2118a, osa-MIR2118b, osa-MIR2118c, osa-MIR2118d, osa-MIR2118e, osa-MIR2118f, osa-MIR2118g, osa-MIR2118h, osa-MIR2118i, osa-MIR2118j, osa-MIR2118k, osa-MIR2118l, osa-MIR2118m, osa-MIR2118n, osa-MIR2118o, osa-MIR2118p, osa-MIR2118q, osa-MIR2118r, osa-MIR2275a, osa-MIR2275b, zma-MIR2118a, zma-MIR2118b, zma-MIR2118c, zma-MIR2118d, zma-MIR2118e, zma-MIR2118f, zma-MIR2118g, zma-MIR2275a, zma-MIR2275b, zma-MIR2275c, zma-MIR2275d, zma-MIR156l, zma-MIR159e, zma-MIR159f, zma-MIR159g, zma-MIR159h, zma-MIR159i, zma-MIR159j, zma-MIR159k, zma-MIR160g, zma-MIR164e, zma-MIR164f, zma-MIR164g, zma-MIR164h, zma-MIR166n, zma-MIR167j, zma-MIR169l, zma-MIR169m, zma-MIR169n, zma-MIR169o, zma-MIR169p, zma-MIR169q, zma-MIR169r, zma-MIR171l, zma-MIR171m, zma-MIR171n, zma-MIR393b, zma-MIR393c, zma-MIR395d, zma-MIR395e, zma-MIR395f, zma-MIR395g, zma-MIR395h, zma-MIR395i, zma-MIR395j, zma-MIR395k, zma-MIR395l, zma-MIR395m, zma-MIR395n, zma-MIR395o, zma-MIR395p, zma-MIR482, zma-MIR528a, zma-MIR528b, zma-MIR529, zma-MIR827, zma-MIR1432, osa-MIR395x, osa-MIR395y, osa-MIR2275c, osa-MIR2275d, ath-MIR156i, ath-MIR156j
The largest miRNA family size identified was miR166 that consisted of 14 members and miR156, miR169 and miR167 possessed 12, 12 and 10 members, respectively; whereas other miRNA families such as miR162, miR529, miR827 and miR1432 had only one member detected in this period. [score:1]
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[+] score: 1
Other miRNAs from this paper: osa-MIR162a, osa-MIR162b
npcRNA78 gene contained the miR162 sequence in an alternative intron and corresponded to the MIR162a locus [24]. [score:1]
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[+] score: 1
The best matches indicating likely true homology were obtained for seven miRNA species: miR162, 164, 167, 168, 169, 171, and 172. [score:1]
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[+] score: 1
Trade name Hybrid Lepidopteran-specific insertion event Lepidopteran-specific Bt proteins Non-Bt1P1319R [a] - -Herculex [®]IP1319HR [a] TC1507 Cry1FOptimum [®] Leptra [®]P1319VYHR [a] TC1507 + MON810 + MIR162 Cry1F + Cry1Ab + Vip3Aa20 Non-Bt2DKC6482R [b] - -Genuity [®]VT Double Pro [™]DKC6489VT2P [b] MON89034 Cry1A. [score:1]
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