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13 publications mentioning dre-mir-7a-1

Open access articles that are associated with the species Danio rerio and mention the gene name mir-7a-1. Click the [+] symbols to view sentences that include the gene name, or the word cloud on the right for a summary.

1
[+] score: 239
Intriguingly, miR-7 expression was significantly decreased by these secretagogues (Supplementary Fig. 4), suggesting an unrecognized molecule or pathway that suppressed the expression of miR-7. This inhibitory effect on miR-7 expression, however, is beneficial for insulin secretion because miR-7 overexpression was found to reduce insulin secretion. [score:13]
Cdr1as upregulates miR-7 target genes expressionFurthermore, we examined whether Cdr1as and/or miR-7 are capable of modulating the expression of endogenous Myrip and Pax6. [score:10]
This observation confirmed that the inhibitory role of Cdr1as in miR-7 is associated with the binding of the target, not the degradation of miR-7 6. Since miR-7 interacts with Cdr1as, we examined whether endogenous miR-7 expression level is affected by forskolin, PMA and glucose treatment under the same condition as used in the stimulation of Cdr1as expression. [score:9]
As shown in the working mo del (Fig. 7), we found that Cdr1as or miR-7 expression was upregulated or downregulated respectively by forskolin and PMA, indicating that Cdr1as/miR-7 is involved in the cAMP and PKC signal pathway. [score:9]
This observation confirmed that the inhibitory role of Cdr1as in miR-7 is associated with the binding of the target, not the degradation of miR-7 6. Since miR-7 interacts with Cdr1as, we examined whether endogenous miR-7 expression level is affected by forskolin, PMA and glucose treatment under the same condition as used in the stimulation of Cdr1as expression. [score:9]
Cdr1as upregulates miR-7 target genes expression. [score:8]
Overexpression of exogenous Cdr1as or stimulation of forskolin or PMA can significantly increase the expression level of Cdr1as in islet cells, which in turn inhibits miR-7’s function in insulin biosynthesis and secretion. [score:7]
These findings clearly showed that Pax-6 levels, as a major target of miR-7, is likely to be upregulated by Cdr1as in islet cells. [score:6]
Although many miR-7 target genes were predicted by miRNA software, only a dozen of them have been experimentally demonstrated to be direct targets in insulin pathway of adult islet β cells. [score:6]
MiR-7 (miR-7a is dominantly expressed in islets) expression plasmid, miRVec-miR-7 and its control plasmid (harboring a scrambled sequence, named as “Ctrl1”) were kindly provided by Dr. [score:5]
miR-7 expression level in islet cells, suggesting most if not all of miR-7 will be inhibited by Cdr1as. [score:5]
Myrip and Pax6 are miR-7 targetsTo explore possible molecular interactions of Cdr1as/miR-7 in islet β cells, we analyzed potential miR-7 targets with two wi dely-used bioinformatics tools, PicTar (http://pictar. [score:5]
Similar results were also observed in freshly isolated mouse islets, (Fig. 3b) i. e., ~30% decrease of insulin secretion in miR-7 overexpression group and ~40% increase in Cdr1as overexpression group (Fig. 3b). [score:5]
Since miR-7 is abundantly expressed in islet cells, we assumed that Cdr1as is also expressed in islet cells and other neuroendocrine tissues. [score:5]
In fact, miR-7 expression was reduced in either glucose or forskolin, whereas Cdr1as expression showed positive response to both secretagogues. [score:5]
Decreased insulin content by miR-7 expression or increased insulin protein by Cdr1as expression was confirmed in MIN6 cells and islet cells (Fig. 4c,d). [score:5]
In order to demonstrate that Myrip is a novel direct target of miR-7, we established two dual-luciferase reporter constructs that contain either a wildtype or a mutated 3′-UTR of Myrip. [score:4]
How to cite this article: Xu, H. et al. The circular RNA Cdr1as, via miR-7 and its targets, regulates insulin transcription and secretion in islet cells. [score:4]
Thereby it was ascertained that miR-7 had an inhibitory effect on Myrip and Pax6 through direct binding of their 3′-UTR. [score:4]
In mouse islets cells, however, overexpression of ciRS-7 reached ~90% increase of insulin content compared to the control, while the miR-7 expression resulted in ~20% decrease of insulin content (Fig. 4b). [score:4]
These results demonstrate that miR-7 could regulate the expression of Myrip and Pax6. [score:4]
For the sake of strong effects on miR-7 function, Cdr1as could be an important regulator to prevent miR-7 from interacting with target transcripts in islet cells. [score:4]
We first confirmed that the Cdr1as expression has direct inhibition on miR-7 activity in islet cells by measuring luciferase reporter activities with constructs either containing miR-7 binding site or the entire ciRS-7 sequence (Supplementary Fig. 1). [score:4]
Also, Pax6 (paired box 6) is a transcriptional factor regulating insulin biosynthesis and secretion 33 34, and a target of miR-7 reported previously 29. [score:4]
To explore possible molecular interactions of Cdr1as/miR-7 in islet β cells, we analyzed potential miR-7 targets with two wi dely-used bioinformatics tools, PicTar (http://pictar. [score:3]
Also, the identification of miR-7 target genes related to insulin signaling pathway in islet β cells may reveal the molecular network responsible for the insulin secretion and homeostasis. [score:3]
Expression levels of Cdr1as and miR-7 are normalized to Gapdh mRNA levels. [score:3]
As expected, miR-7 overexpression reduced ~20% insulin secretion in MIN6 cells. [score:3]
Furthermore, we examined whether Cdr1as and/or miR-7 are capable of modulating the expression of endogenous Myrip and Pax6. [score:3]
Using the insulin ELISA assay, we found that ~25% reduction of insulin content in miR-7 overexpressed MIN6 cells compared to a ~70% increase of insulin content in Cdr1as overexpressed MIN6 cells (Fig. 4a). [score:3]
Each of the construct DNAs was co -transfected with miR-7 expression vector or control vector into 293T cells. [score:3]
Since insulin content was increased by the Cdr1as treatment, we examined whether overexpression of Cdr1as and/or miR-7 affects endogenous insulin1 and insulin2 mRNA levels. [score:3]
Concomitantly, the expression pattern of miR-7 was largely similar to that of Cdr1as, but its transcriptional level in islet or pituitary gland was much higher than Cdr1as (Fig. 1a). [score:3]
In addition, the interaction of Cdr1as with miR-7 could be targeted by another miRNA, miR-671, which triggers endonucleolytic cleavage of Cdr1as 25. [score:3]
Altogether, our results indicate that the effect of Cdr1as on insulin content is through insulin biosynthesis, in which potential target genes of miR-7 may actually play an important role. [score:3]
Myrip and Pax6 are miR-7 targets. [score:3]
As we mentioned earlier, transgenic mice overexpressing miR-7 in β cells developed diabetes due to impaired insulin secretion and β cell dedifferentiation. [score:3]
Apparently, the inactivation of miR-7 in mouse islets showed broad effects on insulin pathway due to alterations of hundreds of potential targets. [score:3]
Overexpression of Cdr1as in islet cells was predicted to result in alterations of insulin secretion because of the function of miR-7 as we discussed above. [score:3]
As determined by qRT-PCR, ~30% and ~20% reduction of insulin 1 and insulin 2 gene respectively were observed in the miR-7 overexpressed MIN6 cells (Fig. 5a). [score:3]
Western blots confirmed that miR-7 reduced Myrip and Pax6 protein levels, while Cdr1as dramatically increased their expression in MIN6 cells (Fig. 6c). [score:3]
Therefore, additional miR-7 targets involving insulin granule metabolism as well as insulin homeostasis should be identified. [score:3]
To identify new targets of miR-7 in insulin secretion pathway, we screened hundreds of candidate genes that were predicted by multiple bioinformatic tools and extensively analyzed the candidate’s function in islet cells. [score:3]
In particular, physiological and biological functions of two major target genes (e. g., Myrip and Pax6) of miR-7 have been well studied in islet cells. [score:3]
Similarly, the endogenous Cdr1as expression level was not altered by exogenous transfection of the miRVec-miR-7 (Supplementary Fig. 3b). [score:3]
This finding is in agreement with the inhibitory role of miR-7 on Pax-6 by ours and others 29. [score:3]
Four plasmids, including Cdr1as expression plasmid, pCDNA3-ciRS-7 and its scrambled sequence plasmid (which inserted a Cdr1as sequence only but no invert repeat flanking introns, resulting in no circular Cdr1as production; named as “Ctrl2”), psiCheck-miR-7 and psiCheck-CiRS-7, were kindly given by Dr. [score:3]
While the forced expression of miR-7 in mouse islet cells showed even more decrease of Myrip mRNA (~50%) and Pax6 mRNA (~60%) compared to their controls (Fig. 6b). [score:2]
Whether these responses are derived from associated promoter elements like CREB binding sites or from indirect elements of the Cdr1as/miR-7 network remains to be studied. [score:2]
MiR-7 expression also responded in a similar way to these secretagogues. [score:2]
Forced expression of miR-7 in MIN6 cells was found to result in ~40% decrease of Myrip mRNA and 50% reduction of Pax6 mRNA compared to the control plasmids -treated cells (Fig. 6b). [score:2]
Furthermore, after miR-7 or Cdr1as plasmid DNA was transfected into MIN6 cells and pancreatic islet cells for 48 h, miR-7 or Cdr1as expression was found to be increased ~70 folds or ~180 folds respectively, compared to the control (Supplementary Fig. 2). [score:2]
However, luciferase activities in the mutant Myrip or the mutant Pax6 did not show significant alterations because the mutations within the seed sequence of Myrip or Pax6 abrogated the binding site of miR-7 (Fig. 6a). [score:2]
In particular, Cdr1as, which is derived from an antisense transcript of the CDR1 protein-coding gene at chromosome Xq27.1, contains 71 binding sites or 26 clusters corresponding to miR-7 sites. [score:1]
Cdr1as and miR-7 plasmid DNAs were separately transfected into MIN6 cells and also dissociated mouse islet cells in culture plates (see details in). [score:1]
Furthermore, an interesting observation is that inactivation of miR-7 in obese mice might be sufficient to rescue β cell failure and glycemia 24. [score:1]
Working mo del of Cdr1as/miR-7 -associated network in β cells. [score:1]
Among multiple hits of miR-7, two interesting genes were prioritized because of their role in insulin biosynthesis and exocytosis. [score:1]
We observed that 40% reduction in the wildtype Myrip transfected cells and 50% reduction in Pax6 transfected cells when co -transfected with miR-7, but not co -transfected with control (Fig. 6a). [score:1]
Interestingly, this interaction between Myrip and MyosinVa was activated by cAMP pathway 39, which is inversely correlated with miR-7 by forskolin treatment observed in this study. [score:1]
293T cells were co -transfected with Myrip or Pax6 3′-UTR luciferase reporter plasmid DNA and miRVec-miR-7 or its control plasmid DNAs at 1:10 ratio and were then harvested after 48 h in culture. [score:1]
Importantly, genetic inactivation of miR-7 in β cells was found to result in increased insulin secretion but not affecting proliferation and apoptosis, indicating that miR-7 is dispensable for the maintenance of endocrine β cell mass 24. [score:1]
Islets cells were transfected with plasmid Ctrl2 or pcDNA3-ciRS-7, together with or without miRVec-miR-7 and psiCheck reporter plasmids. [score:1]
After measuring 3′-UTR luciferase reporter activity of the selected candidate gene, we further confirmed that Myrip expression level was decreased by miR-7 but increased by Cdr1as in the islet cells (Fig. 7). [score:1]
In fact, the increased insulin secretion was shown in both acute phase and second phase in the miR-7 deletion mouse 24. [score:1]
However, the endogenous miR-7 expression level, as measured by qRT-PCR, was found to be unaffected by exogenous transfection of the pCDNA3-CiRS-7 (Supplementary Fig. 3a). [score:1]
These results showed that Cdr1as, as a specific repressor of miR-7, is indeed implicated in the insulin pathway. [score:1]
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[+] score: 54
Other miRNAs from this paper: hsa-let-7a-1, hsa-let-7a-2, hsa-let-7a-3, hsa-let-7b, hsa-let-7c, hsa-let-7d, hsa-let-7e, hsa-let-7f-1, hsa-let-7f-2, hsa-mir-17, hsa-mir-24-1, hsa-mir-24-2, hsa-mir-25, mmu-let-7g, mmu-let-7i, mmu-mir-124-3, mmu-mir-9-2, mmu-mir-134, mmu-mir-137, mmu-mir-138-2, mmu-mir-145a, mmu-mir-24-1, hsa-mir-192, mmu-mir-194-1, mmu-mir-200b, hsa-mir-7-1, hsa-mir-7-2, hsa-mir-7-3, hsa-mir-215, hsa-mir-221, hsa-mir-200b, mmu-mir-296, mmu-let-7d, mmu-mir-106b, hsa-let-7g, hsa-let-7i, hsa-mir-124-1, hsa-mir-124-2, hsa-mir-124-3, hsa-mir-137, hsa-mir-138-2, hsa-mir-145, hsa-mir-9-1, hsa-mir-9-2, hsa-mir-9-3, hsa-mir-134, hsa-mir-138-1, hsa-mir-194-1, mmu-mir-192, mmu-mir-200a, mmu-let-7a-1, mmu-let-7a-2, mmu-let-7b, mmu-let-7c-1, mmu-let-7c-2, mmu-let-7e, mmu-let-7f-1, mmu-let-7f-2, mmu-mir-24-2, mmu-mir-346, hsa-mir-200c, mmu-mir-17, mmu-mir-25, mmu-mir-200c, mmu-mir-221, mmu-mir-124-1, mmu-mir-124-2, mmu-mir-9-1, mmu-mir-9-3, mmu-mir-138-1, mmu-mir-7a-1, mmu-mir-7a-2, mmu-mir-7b, hsa-mir-194-2, mmu-mir-194-2, hsa-mir-106b, hsa-mir-200a, hsa-mir-296, hsa-mir-369, hsa-mir-346, mmu-mir-215, gga-let-7i, gga-let-7a-3, gga-let-7b, gga-let-7c, gga-mir-221, gga-mir-17, gga-mir-138-1, gga-mir-124a, gga-mir-194, gga-mir-215, gga-mir-137, gga-mir-7-2, gga-mir-138-2, gga-let-7g, gga-let-7d, gga-let-7f, gga-let-7a-1, gga-mir-200a, gga-mir-200b, gga-mir-124b, gga-let-7a-2, gga-let-7j, gga-let-7k, gga-mir-7-3, gga-mir-7-1, gga-mir-24, gga-mir-7b, gga-mir-9-2, dre-mir-7b, dre-mir-7a-2, dre-mir-192, dre-mir-221, dre-mir-430a-1, dre-mir-430b-1, dre-mir-430c-1, dre-let-7a-1, dre-let-7a-2, dre-let-7a-3, dre-let-7a-4, dre-let-7a-5, dre-let-7a-6, dre-let-7b, dre-let-7c-1, dre-let-7c-2, dre-let-7d-1, dre-let-7d-2, dre-let-7e, dre-let-7f, dre-let-7g-1, dre-let-7g-2, dre-let-7h, dre-let-7i, dre-mir-7a-3, dre-mir-9-1, dre-mir-9-2, dre-mir-9-4, dre-mir-9-3, dre-mir-9-5, dre-mir-9-6, dre-mir-9-7, dre-mir-17a-1, dre-mir-17a-2, dre-mir-24-4, dre-mir-24-2, dre-mir-24-3, dre-mir-24-1, dre-mir-25, dre-mir-92b, dre-mir-124-1, dre-mir-124-2, dre-mir-124-3, dre-mir-124-4, dre-mir-124-5, dre-mir-124-6, dre-mir-137-1, dre-mir-137-2, dre-mir-138-1, dre-mir-145, dre-mir-194a, dre-mir-194b, dre-mir-200a, dre-mir-200b, dre-mir-200c, dre-mir-430c-2, dre-mir-430c-3, dre-mir-430c-4, dre-mir-430c-5, dre-mir-430c-6, dre-mir-430c-7, dre-mir-430c-8, dre-mir-430c-9, dre-mir-430c-10, dre-mir-430c-11, dre-mir-430c-12, dre-mir-430c-13, dre-mir-430c-14, dre-mir-430c-15, dre-mir-430c-16, dre-mir-430c-17, dre-mir-430c-18, dre-mir-430a-2, dre-mir-430a-3, dre-mir-430a-4, dre-mir-430a-5, dre-mir-430a-6, dre-mir-430a-7, dre-mir-430a-8, dre-mir-430a-9, dre-mir-430a-10, dre-mir-430a-11, dre-mir-430a-12, dre-mir-430a-13, dre-mir-430a-14, dre-mir-430a-15, dre-mir-430a-16, dre-mir-430a-17, dre-mir-430a-18, dre-mir-430i-1, dre-mir-430i-2, dre-mir-430i-3, dre-mir-430b-2, dre-mir-430b-3, dre-mir-430b-4, dre-mir-430b-6, dre-mir-430b-7, dre-mir-430b-8, dre-mir-430b-9, dre-mir-430b-10, dre-mir-430b-11, dre-mir-430b-12, dre-mir-430b-13, dre-mir-430b-14, dre-mir-430b-15, dre-mir-430b-16, dre-mir-430b-17, dre-mir-430b-18, dre-mir-430b-5, dre-mir-430b-19, dre-mir-430b-20, mmu-mir-470, hsa-mir-485, hsa-mir-496, dre-let-7j, mmu-mir-485, mmu-mir-543, mmu-mir-369, hsa-mir-92b, gga-mir-9-1, hsa-mir-671, mmu-mir-671, mmu-mir-496a, mmu-mir-92b, hsa-mir-543, gga-mir-124a-2, mmu-mir-145b, mmu-let-7j, mmu-mir-496b, mmu-let-7k, gga-mir-124c, gga-mir-9-3, gga-mir-145, dre-mir-138-2, dre-mir-24b, gga-mir-9-4, mmu-mir-9b-2, mmu-mir-124b, mmu-mir-9b-1, mmu-mir-9b-3, gga-mir-9b-1, gga-let-7l-1, gga-let-7l-2, gga-mir-9b-2
However, more functional analysis of naturally expressed circRNAs within the CNS will provide useful information regarding the precise role of circRNAs and other long-ncRNAs in the regulation of gene expression required through the different developmental stages of the CNS and also, whether miRNAs other than miR-7 are regulated by long-ncRNAs. [score:8]
This observation suggest that miR-671 might function as an indirect regulator of miR-7 activity by targeting and reducing ciRS-7 levels; however, the exact function of the ciRS-7:miR-671 interaction during the development of the CNS is still unknown. [score:6]
Moreover, due to the high degree of conservation of miR-7, the binding sites in the human CDR1as are functional when it is expressed in zebrafish resulting in impaired midbrain development which is similar to the phenotype of knocking-down miR-7 (Memczak et al., 2013). [score:5]
Interestingly, the concentration gradient of a single miRNA is capable of determining specific zones of neuronal differentiation as it is the case of miR-7 that maintains the proper localization of dopaminergic neuronal differentiation regions within the mouse olfactory bulb by having an opposite concentration/expression gradient to that of its target gene, Pax6 (De Chevigny et al., 2012). [score:5]
Silencing QKI in the U343 glioblastoma cell line, results in miR-7 expression and cell cycle arrest, through a mechanism involving miR-7 negative regulation of epidermal growth factor (EGF) receptor (EGFR) protein levels, thus blunting the EGF -dependent ERK activation (Wang et al., 2013). [score:4]
In particular, the human circRNA antisense to the cerebellar degeneration-related protein 1 transcript (CDR1as) contains 63 conserved binding sites for miR-7 and specifically regulates miR-7 expression in neuronal tissues (Memczak et al., 2013). [score:4]
ciRS-7 contains more than 70 conserved binding sites for miR-7 and when miR-7 binds to it, AGO is recruited and binds to ciRS-7:miR-7 complexes however, ciRS-7 is resistant to miR-7 -mediated destabilization resulting in miR-7 activity blockage and derepression of miR-7 target genes (Hansen et al., 2013). [score:3]
Moreover, knock-out mice for MSI2 present higher levels of mature miR-7 without a change in pri-miR-7 abundance confirming that RBPs are key players in the regulatory mechanism controlling miRNA biogenesis (Choudhury et al., 2013). [score:3]
Locked nucleic acid -based in situ hybridisation reveals miR-7a as a hypothalamus-enriched microRNA with a distinct expression pattern. [score:3]
A recent report, demonstrated that the processing of the miR-7 pre-miRNA generated from the heterogeneous nuclear ribonucleoprotein K (hnRNP K) pre-mRNA transcript, is inhibited in non-brain human and mouse cells due to the binding of the RNA binding proteins (RBPs) Musashi homolog 2 (MSI2) and Hu antigen R (HuR) to the terminal loop of the pri-miR-7 and the stabilization of the pri-miRNA structure (Choudhury et al., 2013). [score:3]
Absence of QKI-5 and QKI-6 results in increased mature miR-7 levels due to the fact that these proteins negatively regulate pri-miR-7 to miR-7 processing by maintaining the pri-miR-7 at the nucleus and tightly bounded by Drosha (Wang et al., 2013). [score:2]
In addition, miR-7 biogenesis regulation also occurs via MSI2 and HuR binding during the in vitro neuronal differentiation of the SH-SY5Y cell line (Choudhury et al., 2013). [score:2]
miR-7a regulation of Pax6 controls spatial origin of forebrain dopaminergic neurons. [score:2]
Another study showed that the control of miR-7 biogenesis by the quaking (QKI) RBPs, isoforms QKI-5 and QKI-6 that are localized at the nucleus and throughout the cell respectively, contribute to regulate the proliferation rate of glioblastoma cells cultures (Wang et al., 2013). [score:2]
An independent study, described ciRS-7, another circRNA, as a miR-7 sponge in the human brain and in mouse neocortical and hippocampal neurons (Hansen et al., 2013). [score:1]
Tissue-specific control of brain-enriched miR-7 biogenesis. [score:1]
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[+] score: 29
Moreover, miR-7 silencing experiments, performed with anti-miR-7 morpholinos, demonstrate an increase in the expression of spata2 mRNA (in ex vivo cultured islets or in the pancreatic β-cell line MIN6) thus confirming that spata2 is down-regulated by miR-7. Since spata2 and miR-7 transcript expression seems to be inversely correlated, it has been proposed that spata2/miR-7 could be involved in islet biogenesis [20]. [score:8]
Bravo-Egana et al. performed a differential expression analysis of miRNAs in human adult acinar and islets pancreatic tissue and identified miR-7 to be the most highly and selectively expressed miRNA in islets, with an islet/acinar ratio of expression greater than 200 [17]. [score:7]
The RNA target predictive algorithms TargetScan, Miranda and PicTar identified spata2 gene as the miR-7 target with the highest rank. [score:7]
Other studies confirmed the major expression of miR-7 during human pancreatic islet development and differentiation and revealed the correlation between its expression and the observed increase in pro-insulin gene transcripts [18, 19]. [score:6]
THE LAST PART OF THE STORY: SPATA2, MIR-7 AND PANCREATIC ISLETS. [score:1]
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[+] score: 20
Other miRNAs from this paper: hsa-let-7a-1, hsa-let-7a-2, hsa-let-7a-3, hsa-let-7b, hsa-let-7e, hsa-mir-20a, hsa-mir-21, hsa-mir-23a, hsa-mir-24-1, hsa-mir-24-2, hsa-mir-26b, hsa-mir-27a, hsa-mir-29a, hsa-mir-31, hsa-mir-29b-1, hsa-mir-29b-2, hsa-mir-103a-2, hsa-mir-103a-1, hsa-mir-199a-1, hsa-mir-148a, hsa-mir-7-1, hsa-mir-7-2, hsa-mir-7-3, hsa-mir-10b, hsa-mir-181a-2, hsa-mir-181b-1, hsa-mir-181c, hsa-mir-199a-2, hsa-mir-199b, hsa-mir-203a, hsa-mir-204, hsa-mir-212, hsa-mir-181a-1, hsa-mir-221, hsa-mir-23b, hsa-mir-27b, hsa-mir-128-1, hsa-mir-132, hsa-mir-133a-1, hsa-mir-133a-2, hsa-mir-143, hsa-mir-200c, hsa-mir-181b-2, hsa-mir-128-2, hsa-mir-200a, hsa-mir-30e, hsa-mir-148b, hsa-mir-338, hsa-mir-133b, dre-mir-7b, dre-mir-7a-2, dre-mir-10b-1, dre-mir-181b-1, dre-mir-181b-2, dre-mir-199-1, dre-mir-199-2, dre-mir-199-3, dre-mir-203a, dre-mir-204-1, dre-mir-181a-1, dre-mir-221, dre-mir-222a, dre-let-7a-1, dre-let-7a-2, dre-let-7a-3, dre-let-7a-4, dre-let-7a-5, dre-let-7a-6, dre-let-7b, dre-let-7e, dre-mir-7a-3, dre-mir-10b-2, dre-mir-20a, dre-mir-21-1, dre-mir-21-2, dre-mir-23a-1, dre-mir-23a-2, dre-mir-23a-3, dre-mir-23b, dre-mir-24-4, dre-mir-24-2, dre-mir-24-3, dre-mir-24-1, dre-mir-26b, dre-mir-27a, dre-mir-27b, dre-mir-29b-1, dre-mir-29b-2, dre-mir-29a, dre-mir-30e-2, dre-mir-101b, dre-mir-103, dre-mir-128-1, dre-mir-128-2, dre-mir-132-1, dre-mir-132-2, dre-mir-133a-2, dre-mir-133a-1, dre-mir-133b, dre-mir-133c, dre-mir-143, dre-mir-148, dre-mir-181c, dre-mir-200a, dre-mir-200c, dre-mir-203b, dre-mir-204-2, dre-mir-338-1, dre-mir-338-2, dre-mir-454b, hsa-mir-181d, dre-mir-212, dre-mir-181a-2, hsa-mir-551a, hsa-mir-551b, dre-mir-31, dre-mir-722, dre-mir-724, dre-mir-725, dre-mir-735, dre-mir-740, hsa-mir-103b-1, hsa-mir-103b-2, dre-mir-2184, hsa-mir-203b, dre-mir-7146, dre-mir-181a-4, dre-mir-181a-3, dre-mir-181a-5, dre-mir-181b-3, dre-mir-181d, dre-mir-204-3, dre-mir-24b, dre-mir-7133, dre-mir-128-3, dre-mir-7132, dre-mir-338-3
For instance, while miR-181b and miR-7 levels were highly upregulated in injured zebrafish and bichir fins, analysis of regenerating axolotl forelimbs showed expression levels were significantly downregulated. [score:9]
S22 Table Zebrafish Ensembl gene identifiers for 58 genes downregulated in three mo dels with predicted miRNA binding sites for miR-21, miR-181c, miR-181b, miR-31 and miR-7 and members of the network of commonly up- and downregulated genes with functional interactions to 11 blastema -associated genes. [score:7]
S21 Table Zebrafish Ensembl gene identifiers for 136 genes downregulated in three mo dels with predicted miRNA binding sites for miR-21, miR-181c, miR-181b, miR-31 or miR-7 in all three mo dels. [score:4]
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[+] score: 9
Specifically, tumor suppressor miRNAs, such as miR-23a, miR-26a/b, miR-29a/b and miR-101a, were found upregulated, whereas oncogenic miRNAs, like miR-7a and members of the miR cluster 17∼92, were downregulated [34]. [score:9]
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6
[+] score: 9
Other miRNAs from this paper: dre-mir-7b, dre-mir-7a-2, dre-mir-34a, dre-mir-181b-1, dre-mir-181b-2, dre-mir-182, dre-mir-183, dre-mir-181a-1, dre-mir-219-1, dre-mir-219-2, dre-mir-221, dre-mir-222a, dre-mir-430a-1, dre-mir-430b-1, dre-mir-430c-1, dre-let-7a-1, dre-let-7a-2, dre-let-7a-3, dre-let-7a-4, dre-let-7a-5, dre-let-7a-6, dre-let-7b, dre-let-7c-1, dre-let-7c-2, dre-let-7d-1, dre-let-7d-2, dre-let-7e, dre-let-7f, dre-let-7g-1, dre-let-7g-2, dre-let-7h, dre-let-7i, dre-mir-7a-3, dre-mir-9-1, dre-mir-9-2, dre-mir-9-4, dre-mir-9-3, dre-mir-9-5, dre-mir-9-6, dre-mir-9-7, dre-mir-92b, dre-mir-96, dre-mir-100-1, dre-mir-100-2, dre-mir-124-1, dre-mir-124-2, dre-mir-124-3, dre-mir-124-4, dre-mir-124-5, dre-mir-124-6, dre-mir-125b-1, dre-mir-125b-2, dre-mir-125b-3, dre-mir-128-1, dre-mir-128-2, dre-mir-132-1, dre-mir-132-2, dre-mir-135c-1, dre-mir-135c-2, dre-mir-137-1, dre-mir-137-2, dre-mir-138-1, dre-mir-153a, dre-mir-181c, dre-mir-200a, dre-mir-218a-1, dre-mir-218a-2, dre-mir-219-3, dre-mir-375-1, dre-mir-375-2, dre-mir-454a, dre-mir-430c-2, dre-mir-430c-3, dre-mir-430c-4, dre-mir-430c-5, dre-mir-430c-6, dre-mir-430c-7, dre-mir-430c-8, dre-mir-430c-9, dre-mir-430c-10, dre-mir-430c-11, dre-mir-430c-12, dre-mir-430c-13, dre-mir-430c-14, dre-mir-430c-15, dre-mir-430c-16, dre-mir-430c-17, dre-mir-430c-18, dre-mir-430a-2, dre-mir-430a-3, dre-mir-430a-4, dre-mir-430a-5, dre-mir-430a-6, dre-mir-430a-7, dre-mir-430a-8, dre-mir-430a-9, dre-mir-430a-10, dre-mir-430a-11, dre-mir-430a-12, dre-mir-430a-13, dre-mir-430a-14, dre-mir-430a-15, dre-mir-430a-16, dre-mir-430a-17, dre-mir-430a-18, dre-mir-430i-1, dre-mir-430i-2, dre-mir-430i-3, dre-mir-430b-2, dre-mir-430b-3, dre-mir-430b-4, dre-mir-430b-6, dre-mir-430b-7, dre-mir-430b-8, dre-mir-430b-9, dre-mir-430b-10, dre-mir-430b-11, dre-mir-430b-12, dre-mir-430b-13, dre-mir-430b-14, dre-mir-430b-15, dre-mir-430b-16, dre-mir-430b-17, dre-mir-430b-18, dre-mir-430b-5, dre-mir-430b-19, dre-mir-430b-20, dre-let-7j, dre-mir-181a-2, dre-mir-34b, dre-mir-34c, dre-mir-222b, dre-mir-138-2, dre-mir-181a-4, dre-mir-181a-3, dre-mir-181a-5, dre-mir-181b-3, dre-mir-181d, dre-mir-128-3
Additional data file 1 is a figure showing miR-7 expression in the zebrafish brain. [score:3]
Click here for file miR-7 expression in the zebrafish brain. [score:3]
miR-7 expression in the zebrafish brain. [score:3]
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7
[+] score: 5
We showed by qRT-PCR analysis that the expression levels of miR-219, miR-190b, miR-19a, miR-7a and miR-7b significantly increase as a consequence of Tbx5 overexpression (Fig. 1E). [score:5]
[1 to 20 of 1 sentences]
8
[+] score: 5
The expression level of miR-137, miR-724, miR-7a and miR-734 was more than 10 fold higher in silver than in bighead carp, while for miR-196b, the expression level was more than 10 fold higher in bighead carp than in silver carp. [score:5]
[1 to 20 of 1 sentences]
9
[+] score: 4
Other miRNAs from this paper: dre-mir-7b, dre-mir-7a-2, dre-mir-182, dre-mir-183, dre-mir-205, dre-mir-214, dre-mir-430a-1, dre-mir-430b-1, dre-mir-430c-1, dre-let-7a-1, dre-let-7a-2, dre-let-7a-3, dre-let-7a-4, dre-let-7a-5, dre-let-7a-6, dre-let-7b, dre-let-7c-1, dre-let-7c-2, dre-let-7d-1, dre-let-7d-2, dre-let-7e, dre-let-7f, dre-let-7g-1, dre-let-7g-2, dre-let-7h, dre-let-7i, dre-mir-1-2, dre-mir-1-1, dre-mir-7a-3, dre-mir-30c, dre-mir-124-1, dre-mir-124-2, dre-mir-124-3, dre-mir-124-4, dre-mir-124-5, dre-mir-124-6, dre-mir-140, dre-mir-206-1, dre-mir-206-2, dre-mir-375-1, dre-mir-375-2, dre-mir-430c-2, dre-mir-430c-3, dre-mir-430c-4, dre-mir-430c-5, dre-mir-430c-6, dre-mir-430c-7, dre-mir-430c-8, dre-mir-430c-9, dre-mir-430c-10, dre-mir-430c-11, dre-mir-430c-12, dre-mir-430c-13, dre-mir-430c-14, dre-mir-430c-15, dre-mir-430c-16, dre-mir-430c-17, dre-mir-430c-18, dre-mir-430a-2, dre-mir-430a-3, dre-mir-430a-4, dre-mir-430a-5, dre-mir-430a-6, dre-mir-430a-7, dre-mir-430a-8, dre-mir-430a-9, dre-mir-430a-10, dre-mir-430a-11, dre-mir-430a-12, dre-mir-430a-13, dre-mir-430a-14, dre-mir-430a-15, dre-mir-430a-16, dre-mir-430a-17, dre-mir-430a-18, dre-mir-430i-1, dre-mir-430i-2, dre-mir-430i-3, dre-mir-430b-2, dre-mir-430b-3, dre-mir-430b-4, dre-mir-430b-6, dre-mir-430b-7, dre-mir-430b-8, dre-mir-430b-9, dre-mir-430b-10, dre-mir-430b-11, dre-mir-430b-12, dre-mir-430b-13, dre-mir-430b-14, dre-mir-430b-15, dre-mir-430b-16, dre-mir-430b-17, dre-mir-430b-18, dre-mir-430b-5, dre-mir-430b-19, dre-mir-430b-20, dre-let-7j
Thus, knockdown of such miRNAs might not dramatically affect gene expression, but ensure robustness of protein interaction networks as for example miR-7 in Drosophila [33]. [score:4]
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10
[+] score: 3
CDR1 antisense transcript (CDR1as) is a circular RNA in mouse and human brain that contains more than 70 binding sites for the microRNA miR-7 and may suppress its activity. [score:3]
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11
[+] score: 2
Other miRNAs from this paper: mmu-let-7g, mmu-let-7i, mmu-mir-124-3, mmu-mir-140, mmu-mir-141, mmu-mir-152, mmu-mir-182, mmu-mir-183, mmu-mir-191, mmu-mir-199a-1, mmu-mir-200b, mmu-mir-205, mmu-let-7d, mmu-mir-200a, mmu-let-7a-1, mmu-let-7a-2, mmu-let-7b, mmu-let-7c-1, mmu-let-7c-2, mmu-let-7e, mmu-let-7f-1, mmu-let-7f-2, mmu-mir-96, mmu-mir-200c, mmu-mir-214, mmu-mir-199a-2, mmu-mir-199b, mmu-mir-124-1, mmu-mir-124-2, mmu-mir-7a-1, mmu-mir-7a-2, mmu-mir-7b, dre-mir-7b, dre-mir-7a-2, dre-mir-182, dre-mir-183, dre-mir-199-1, dre-mir-199-2, dre-mir-199-3, dre-mir-205, dre-mir-214, dre-mir-430a-1, dre-mir-430b-1, dre-mir-430c-1, mmu-mir-429, mmu-mir-449a, dre-mir-429a, dre-let-7a-1, dre-let-7a-2, dre-let-7a-3, dre-let-7a-4, dre-let-7a-5, dre-let-7a-6, dre-let-7b, dre-let-7c-1, dre-let-7c-2, dre-let-7d-1, dre-let-7d-2, dre-let-7e, dre-let-7f, dre-let-7g-1, dre-let-7g-2, dre-let-7h, dre-let-7i, dre-mir-7a-3, dre-mir-96, dre-mir-124-1, dre-mir-124-2, dre-mir-124-3, dre-mir-124-4, dre-mir-124-5, dre-mir-124-6, dre-mir-140, dre-mir-141, dre-mir-152, dre-mir-200a, dre-mir-200b, dre-mir-200c, dre-mir-430c-2, dre-mir-430c-3, dre-mir-430c-4, dre-mir-430c-5, dre-mir-430c-6, dre-mir-430c-7, dre-mir-430c-8, dre-mir-430c-9, dre-mir-430c-10, dre-mir-430c-11, dre-mir-430c-12, dre-mir-430c-13, dre-mir-430c-14, dre-mir-430c-15, dre-mir-430c-16, dre-mir-430c-17, dre-mir-430c-18, dre-mir-430a-2, dre-mir-430a-3, dre-mir-430a-4, dre-mir-430a-5, dre-mir-430a-6, dre-mir-430a-7, dre-mir-430a-8, dre-mir-430a-9, dre-mir-430a-10, dre-mir-430a-11, dre-mir-430a-12, dre-mir-430a-13, dre-mir-430a-14, dre-mir-430a-15, dre-mir-430a-16, dre-mir-430a-17, dre-mir-430a-18, dre-mir-430i-1, dre-mir-430i-2, dre-mir-430i-3, dre-mir-430b-2, dre-mir-430b-3, dre-mir-430b-4, dre-mir-430b-6, dre-mir-430b-7, dre-mir-430b-8, dre-mir-430b-9, dre-mir-430b-10, dre-mir-430b-11, dre-mir-430b-12, dre-mir-430b-13, dre-mir-430b-14, dre-mir-430b-15, dre-mir-430b-16, dre-mir-430b-17, dre-mir-430b-18, dre-mir-430b-5, dre-mir-430b-19, dre-mir-430b-20, dre-let-7j, mmu-mir-449c, mmu-mir-449b, dre-mir-429b, mmu-let-7j, mmu-let-7k, mmu-mir-124b
In Drosophila, miR-7 has been implicated in photoreceptor cell differentiation through regulation of local EGF receptor signaling (Li and Carthew, 2005). [score:2]
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12
[+] score: 2
Other miRNAs from this paper: hsa-let-7a-1, hsa-let-7a-2, hsa-let-7a-3, hsa-let-7b, hsa-let-7c, hsa-let-7d, hsa-let-7e, hsa-let-7f-1, hsa-let-7f-2, hsa-mir-96, mmu-let-7g, mmu-let-7i, mmu-mir-124-3, mmu-mir-9-2, mmu-mir-141, mmu-mir-152, mmu-mir-182, mmu-mir-183, mmu-mir-199a-1, hsa-mir-199a-1, mmu-mir-200b, mmu-mir-205, hsa-mir-7-1, hsa-mir-7-2, hsa-mir-7-3, hsa-mir-182, hsa-mir-183, hsa-mir-199a-2, hsa-mir-199b, hsa-mir-205, hsa-mir-214, hsa-mir-200b, mmu-let-7d, mmu-mir-130b, hsa-let-7g, hsa-let-7i, hsa-mir-124-1, hsa-mir-124-2, hsa-mir-124-3, hsa-mir-141, hsa-mir-152, hsa-mir-9-1, hsa-mir-9-2, hsa-mir-9-3, mmu-mir-200a, mmu-let-7a-1, mmu-let-7a-2, mmu-let-7b, mmu-let-7c-1, mmu-let-7c-2, mmu-let-7e, mmu-let-7f-1, mmu-let-7f-2, mmu-mir-96, hsa-mir-200c, mmu-mir-200c, mmu-mir-214, mmu-mir-199a-2, mmu-mir-199b, mmu-mir-124-1, mmu-mir-124-2, mmu-mir-9-1, mmu-mir-9-3, mmu-mir-7a-1, mmu-mir-7a-2, mmu-mir-7b, hsa-mir-200a, hsa-mir-130b, hsa-mir-376a-1, mmu-mir-376a, dre-mir-7b, dre-mir-7a-2, dre-mir-182, dre-mir-183, dre-mir-199-1, dre-mir-199-2, dre-mir-199-3, dre-mir-205, dre-mir-214, hsa-mir-429, mmu-mir-429, hsa-mir-450a-1, mmu-mir-450a-1, dre-mir-429a, dre-let-7a-1, dre-let-7a-2, dre-let-7a-3, dre-let-7a-4, dre-let-7a-5, dre-let-7a-6, dre-let-7b, dre-let-7c-1, dre-let-7c-2, dre-let-7d-1, dre-let-7d-2, dre-let-7e, dre-let-7f, dre-let-7g-1, dre-let-7g-2, dre-let-7h, dre-let-7i, dre-mir-7a-3, dre-mir-9-1, dre-mir-9-2, dre-mir-9-4, dre-mir-9-3, dre-mir-9-5, dre-mir-9-6, dre-mir-9-7, dre-mir-96, dre-mir-124-1, dre-mir-124-2, dre-mir-124-3, dre-mir-124-4, dre-mir-124-5, dre-mir-124-6, dre-mir-130b, dre-mir-141, dre-mir-152, dre-mir-200a, dre-mir-200b, dre-mir-200c, hsa-mir-450a-2, dre-let-7j, hsa-mir-376a-2, mmu-mir-450a-2, dre-mir-429b, mmu-let-7j, mmu-let-7k, mmu-mir-9b-2, mmu-mir-124b, mmu-mir-9b-1, mmu-mir-9b-3
In Drosophila, miR-7 has been implicated in the differentiation of photoreceptor cells via regulation of the EGF receptor signalling (Li and Carthew, 2005). [score:2]
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13
[+] score: 1
The mutant version of miR-27b-SP (miR-27b-SP-mut) was generated with three nucleotides of the seed binding site sequence for the miR-7 binding site (AC TGTGA) were substituted (AC AAAGA). [score:1]
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