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39 publications mentioning hsa-mir-345

Open access articles that are associated with the species Homo sapiens and mention the gene name mir-345. Click the [+] symbols to view sentences that include the gene name, or the word cloud on the right for a summary.

1
[+] score: 407
Other miRNAs from this paper: hsa-mir-21, hsa-mir-93
To determine that HCV core -induced miR-345 down-regulated endogenous p21 [Waf1/Cip1] gene expression, core expressing HepG2 cells were transfected with or without miR-345 inhibitor, and then p21 [Waf1/Cip1] gene expression at protein level was determined at 24 hours after transfection. [score:12]
Moreover, HCV core -induced miR-345 suppresses p21 [Waf1/Cip1] gene expression in HepG2 cells, and inhibits curcumin -mediated apoptosis through down-regulation of p21 [Waf1/Cip1] gene expression in Huh7 cells. [score:12]
Indeed, we also demonstrated that more truncated core protein (amino acids 1–153) which deletes more residues of hydrophobic C-terminal region up-regulated miR-345 expression, indicating that the up-regulation of miR-345 expression may be associated with the nuclear localization of HCV core protein. [score:11]
MicroRNA-345 down-regulates p21 [Waf1/Cip1] gene expression in human hepatoma cellsIt has been reported that human p21 [Waf1/Cip1] gene expression can be inhibited by miR-345 and miR-93 in HEK 293 cells [33]. [score:10]
In conclusion, our study demonstrates that mature or more truncated HCV core protein (amino acids 1–173 or 1–153) can up-regulate the expression of miR-345 which then suppresses p21 [Waf1/Cip1] gene expression in human hepatoma cells. [score:10]
The HCV core -induced miR-345 suppressed endogenous p21 [Waf1/Cip1] gene expression through targeting its 3′ untranslated region (3′UTR) in HepG2 cells and curcumin-stimulated Huh7 cells. [score:9]
HCV core -induced microRNA-345 suppressed p21 [Waf1/Cip1] gene expression through targeting its 3′ untranslated region in human hepatoma cells. [score:9]
HCV core -induced microRNA-345 inhibits p21 [Waf1/Cip1] gene expression in HepG2 cells and curcumin-stimulated Huh7 cellsIt has been reported that the mature form (amino acids 1–173) of HCV core protein in the nucleus suppresses p21 [Waf1/Cip1] gene expression in HepG2 cells [27]. [score:9]
Similarly, the suppression of p21 [Waf1/Cip1] gene expression was attenuated when mature core -expressing cells were transfected with miR-345 inhibitor (Fig. 4B, upper panel, lane 3, lane 6 and lane 7). [score:9]
These results showed that miR-345 may down-regulate human p21 [Waf1/Cip1] gene expression through targeting its 3′UTR in human hepatoma cells. [score:8]
MicroRNA-345 down-regulates p21 [Waf1/Cip1] gene expression through targeting its 3′UTR but not microRNA-93 in human hepatoma cells. [score:8]
Together, these results demonstrate that miR-345 down-regulates p21 [Waf1/Cip1] gene expression through targeting its 3′UTR in human hepatoma cells. [score:8]
Together, these results demonstrate that miR-345 inhibits curcumin -induced apoptosis through down-regulation of p21 [Waf1/Cip1] gene expression in Huh7 cells. [score:8]
HCV core protein enhances the expression of microRNA-345 which then down-regulates p21 [Waf1/Cip1] expression. [score:8]
In this study, we indicated that two p21 [Waf1/Cip1] -targeting microRNAs, microRNA-345 (miR-345) and microRNA-93 (miR-93), were up-regulated in core -overexpressing Huh7 cells (Fig. 1B). [score:8]
The results showed that miR-345 and miR-93 were overexpressed with more than 3.5- and 2-fold changes, respectively, in mature core (173 a. a. ) and more truncated core (153 a. a. ) -overexpressing Huh7 cells but not in full-length core (191 a. a. ) -overexpressing cells compared to cells nonexpressing core (Fig. 1C, left upper panel). [score:8]
MicroRNA-345 inhibits curcumin -mediated apoptosis through down-regulation of p21 [Waf1/Cip1] gene expression in Huh7 cells. [score:7]
MicroRNA-345 inhibits curcumin -induced apoptosis through down-regulation of p21 [Waf1/Cip1] gene expression in Huh7 cells. [score:7]
MicroRNA-345 inhibits curcumin -induced apoptosis through down-regulation of p21 [Waf1/Cip1] gene expression in Huh7 cellsCurcumin, a potential anticancer agent, has been used in preclinical in vitro and in vivo mo dels of HCC [22]. [score:7]
In conclusion, it is the first time that HCV core protein has ever been demonstrated to inhibit human p21 [Waf1/Cip1] gene expression through miR-345 targeting. [score:7]
MiR-345 has been reported to down-regulate BAG3 gene expression, an anti-apoptosis gene, as a tumor suppressor microRNA in human colorectal cancer [38]. [score:7]
It is the first time that HCV core protein has ever been shown to suppress p21 [Waf1/Cip1] gene expression through miR-345 targeting. [score:7]
However, the suppression of p21 [Waf1/Cip1] gene expression was attenuated in the presence of increased amount of miR-345 inhibitor (Fig. 4A, lane 3, lane 6 and lane 7). [score:7]
Up-regulation of miR-345 expression may involve in cancer development. [score:7]
HCV core protein up-regulates miR-345 and miR-93 expression in human hepatoma cells. [score:6]
The up-regulation of miR-345 expression by HCV core protein may be associated with the demethylation of its promoter. [score:6]
A recent study also reveals that some tumor-related microRNAs including miR-345 are up-regulated from 28 published tumor studies by analyzing microRNA expression microarray datasets, indicating that miR-345 may be an oncomiR in human cancers including HCC [43]. [score:6]
To further verify the down-regulation of endogenous p21 [Waf1/Cip1] gene expression by miR-345, p21 [Waf1/Cip1] mRNA and protein levels were examined in HepG2 cells at 24 hours after transfection with miR-345 mimic. [score:6]
In this study, up-regulation of miR-345 expression by mature form (amino acids 1–173) of the core protein may be also associated with its nuclear localization. [score:6]
The up-regulation of miR-21 expression by mature form (amino acids 1–173) of the core protein may contribute to miR-345 promoter hypomethylation. [score:6]
The HCV core protein was able to up-regulate microRNA-345 (miR-345) expression in human hepatoma cells. [score:6]
Furthermore, we demonstrate that mature HCV core -induced miR-345 involves in anti-apoptosis through down-regulation of p21 [Waf1/Cip1] gene expression in curcumin-stimulated Huh7 cells. [score:6]
Together, these results demonstrate that truncated HCV core proteins (amino acids 1–173 and 1–153) up-regulate cellular miR-345 and miR-93 expression in human hepatoma cells. [score:6]
Our study shows that the up-regulation of miR-345 expression may be related to hepatocarcinogenesis during chronic HCV infection. [score:6]
Some studies have described the up-regulation of miR-345 expression in human cancers including oral squamous cell carcinomas and malignant mesothelioma [41], [42]. [score:6]
However, in our study, miR-345 down-regulates p21 [Waf1/Cip1] gene expression as an oncomiR in human hepatoma cells. [score:6]
MiR-345 is down-regulated in full-length HCV genome -expressing cells as reported by Braconi et al. [19]. [score:5]
Therefore, curcumin was used to induce endogenous p21 [Waf1/Cip1] gene expression to investigate the inhibition of p21 [Waf1/Cip1] gene expression by HCV-core -induced miR-345 in Huh7 cells. [score:5]
As expected, p21 [Waf1/Cip1] gene expression at protein level was suppressed by miR-345 mimic in curcumin-stimulated Huh7 cells in a dose -dependent manner (Fig. 3C, upper panel). [score:5]
Three core gene -expressing vectors for core protein with amino acids 1–191, 1–173 and 1–153, respectively, were transfected into cells, and then the relative expression of miR-345 and miR-93 was determined at 48 hours after transfection. [score:5]
MicroRNA-345 and microRNA-93 are overexpressed in HCV core -overexpressing human hepatoma cells. [score:5]
Moreover, the core protein inhibited curcumin -induced apoptosis through p21 [Waf1/Cip1] -targeting microRNA-345 in Huh7 cells. [score:5]
MicroRNA-345 down-regulates p21 [Waf1/Cip1] gene expression in human hepatoma cells. [score:5]
It has been reported that human p21 [Waf1/Cip1] gene expression can be inhibited by miR-345 and miR-93 in HEK 293 cells [33]. [score:5]
0061089.g004 Figure 4HCV core -induced microRNA-345 inhibits p21 [Waf1/Cip1] gene expression in HepG2 cells and curcumin-stimulated Huh7 cells. [score:5]
We also show that mature HCV core -induced miR-345 can suppress endogenous p21 [Waf1/Cip1] gene expression in HepG2 and curcumin-stimulated Huh7 cells. [score:5]
Together, these results demonstrate that HCV core -induced miR-345 inhibits endogenous p21 [Waf1/Cip1] gene expression in HepG2 cells and curcumin-stimulated Huh7 cells. [score:5]
In addition, the core protein inhibited curcumin -induced apoptosis through p21 [Waf1/Cip1] -targeting miR-345 in Huh7 cells. [score:5]
The relative expressions of miR-345 and miR-93 were determined by TaqMan real-time qPCR in full-length HCV replicon -expressing Huh7 cells (right lower panel). [score:5]
HCV core -induced microRNA-345 inhibits p21 [Waf1/Cip1] gene expression in HepG2 cells and curcumin-stimulated Huh7 cells. [score:5]
Additionally, the suppression of curcumin -induced apoptosis by mature core protein (amino acids 1–173) was significantly attenuated in the presence of increased amount of miR-345 inhibitor (Fig. 4B, middle and lower panels). [score:5]
As expected, p21 [Waf1/Cip1] gene expression at mRNA and protein levels was suppressed with the increased amount of miR-345 mimic in HepG2 cells (Fig. 2C, left and right panels). [score:5]
Figure S2 Curcumin has no effect on endogenous miR-345 expression. [score:3]
Because the different cell types might generate different results, the effects of miR-345 and miR-93 on human p21 [Waf1/Cip1] gene expression were examined in human hepatoma cells. [score:3]
The results showed that wild-type miR-345 mimic can significantly inhibit the luciferase activity in wild-type p21 3′UTR construct -transfected Huh7 cells but not in mutant p21 3′UTR construct -transfected cells (Fig. 2B, right panel, second and third bar pairs). [score:3]
The results showed that the treatment with miR-345 mimic led to a significant reduction of luciferase activity in p21 3′UTR Sense construct -transfected Huh7 cells, but treatment with miR-93 mimic had no significant inhibition in luciferase activity (Fig. 2A, left lower panel, second bar cluster). [score:3]
The relative expressions of miR-345 and miR-93 were determined by TaqMan real-time qPCR. [score:3]
Curcumin -induced apoptosis was inhibited with increased amount of miR-345 mimic (Fig. 3C, left middle panel). [score:3]
However, an additional experiment with mutant miR-345 mimic (Fig. 2B, left panel, mutated sites are underlined) and mutant p21 3′UTR with restoring complementarity was able to show a significant inhibition of luciferase activity (Fig. 2B, right panel, fourth bar pair). [score:3]
Curcumin had no effect on the modulation of cellular miR-345 expression in Huh7 cells (Fig. S2). [score:3]
The relative expression of miR-345 was determined by TaqMan real-time qPCR. [score:3]
At 48 hours after transfection, relative expression of miR-345 or miR-93 was determined by TaqMan real-time qPCR in Huh7 cells (left upper panel) and HepG2 cells (right upper panel). [score:3]
Human miR-345 inhibitor (MH12733) was purchased from Applied Biosystems. [score:3]
The results showed that miR-345 induction was not significantly affected in full-length HCV replicon -expressing system in Huh7 cells compared to control cells and untreated cells (Fig. 1D, right lower panel, third bar pair). [score:2]
Human microRNA-345 (hsa-miR-345, MIMAT0000772) and microRNA-93 (hsa-miR-93, MIMAT0000093) mimics were purchased from Thermo Scientific. [score:1]
Moreover, treatment with a mixture of miR-345 and miR-93 mimics also had no double reduction of luciferase activity (Fig. 2A, left lower panel, second bar cluster). [score:1]
Mutant miR-345 mimic was synthesized. [score:1]
Mutant microRNA-345 mimic was synthesized by Thermo Scientific. [score:1]
Huh7 cells and HepG2 cells were transfected with p21 3′UTR sense or antisense luciferase reporter vector in combination with miR-345 mimic, miR-93 mimic or a mixture of miR-345 and miR-93 mimics. [score:1]
Three microRNAs, miR-21, miR-345 and miR93, of thirty-one microRNAs were indicated. [score:1]
Array data were further confirmed by TaqMan real-time qPCR for miR-345 and miR-93 in Huh7 and HepG2 cells. [score:1]
The mutant p21 [Waf1/Cip1] 3′UTR in the seed sequence of hsa-miR-345 was generated by PCR and ligation of two pieces of DNA fragments, and then cloned into pGL3-Control vector to yield a pGL3-Control-p21 3′UTR Mutant vector. [score:1]
Huh7 cells were transfected with wild-type p21 3′UTR sense or mutant p21 3′UTR luciferase reporter vector in combination with wild-type or mutant miR-345 mimic. [score:1]
As expected, miR-345 and miR-93 mimics had no effect in control vector -transfected and p21 3′UTR Antisense construct -transfected Huh7 cells (Fig. 2A, left lower panel, first and third bar clusters). [score:1]
The number of apoptotic cells (early and late apoptotic cells) had a maximum reduction of about 55% when curcumin-stimulated cells were transfected with 50 nM miR-345 mimic (Fig. 3C, left lower and right lower panels). [score:1]
We further focused on miR-345 to identify the seed sequence in human p21 [Waf1/Cip1] 3′UTR by using computational tools, miRanda (http://www. [score:1]
One luciferase reporter vector, pGL3-Control-p21 3′UTR Mutant (mutated in the seed sequence of miR-345), was constructed. [score:1]
The experiment with mutant miR-345 mimic and mutant p21 3′UTR with restoring complementarity was performed (right panel). [score:1]
Tang et al. has demonstrated that miR-345 with a CpG island in the promoter is a methylation-sensitive microRNA and is highly induced by demethylating agent in human colorectal cancer cell lines [38]. [score:1]
Moreover, the introduction of miR-345 mimic enhanced cell viability in curcumin-stimulated Huh7 cells (Fig. 3C, right middle panel). [score:1]
In this study, we determine the effect of a mature HCV core protein on miR-345 induction in human hepatoma cells. [score:1]
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2
[+] score: 34
Among these upregulated miRNAs were miR-10a, which is a candidate tumor suppressor and suppresses apoptosis in leukemia [39], miR-409 that suppresses tumor cell invasion and metastasis in gastric cancer [40], and miR-206 and miR-345, which are frequently downregulated in various types of cancers and are believed to act as tumor suppressors [41], [42]. [score:15]
As shown in Figure 9C, there was excellent concordance in the data from the miRNA profiling and qPCR, the expression of miR-21, miR-26a, miR-24, miR-30b and miR-29a was down-regulated by EF24 treatment both in vitro and in vivo, while the expression of miR-345, miR-409, miR-10a and miR-206 was upregulated by EF24 treatment. [score:11]
Only four miRNAs (miR-10a, miR-409, miR-206 and miR-345) were upregulated both in vitro and in vivo, which reportedly act as tumor suppressors or inhibitors of cell cycle progression. [score:8]
[1 to 20 of 3 sentences]
3
[+] score: 32
We have shown that in tumor samples compared to normal samples, the majority of miRNAs (miR-216, miR-217, miR-100, miR-345, miR-141, miR-483-3p, miR-26b, miR-150, Let-7b, Let-195 and miR-96) were downregulated, and few were upregulated (miR-146b, miR-205, miR-31, miR-192, miR-194 21, miR-379, miR-431, miR-541, and miR-199b). [score:6]
However, a recent report in colorectal cancer patient tissues has shown significantly methylated mir-345 promoter region compared to the non-cancerous, adenoma, and normal colon tissues; further, ectopic over -expression of mir-345 in colorectal cancer cells significantly reduced cell proliferation by inhibiting the translation of anti-apoptotic BAG3 gene [51]. [score:6]
Similarly, the expression of miR-345 and miR-96 were significantly downregulated during the progression of PC in the mouse mo del (Figure 2D), which is consistent with previous reports [39, 40, 50]. [score:6]
Results showed the downregulation of miR-345, miR-96, and Let-7b in the majority of the PC cell lines compared to the HPDE cells (Figure 3A), indicating that these miRNAs were expressed in ductal cells rather than in tumor stroma. [score:5]
Further, at 50 weeks of age, the expression of miR-216, miR-217, miR-345, miR-141, miR-483-3p, miR-26b, miR-96, Let-7b (p-value = 0.01), miR-100, miR-26a and miR-150 (p-value = 0.094) were further downregulated in KC animals compared to control mice (Figure 2D). [score:5]
These studies suggest a pro-apoptotic function of miR-345; its downregulation during PC progression may be associated with promoter methylation. [score:4]
[1 to 20 of 6 sentences]
4
[+] score: 10
control Expression in case group 1 hsa-mir-1290 0.26 down 2 hsa-mir-342-5p 0.22 down 3 hsa-mir-1224-5p 0.23 down 4 hsa-mir-345 0.38 down 5 hsa-mir-1228 0.38 down 6 hsa-mir-1249 0.32 down 7 hsa-mir-1826 0.26 down 8 hsa-miR-1306 0.38 down 9 hsa-miR-188-5p 0.43 down 10 hsa-miR-320a 0.48 down 11 hsa-miR-320c 0.26 down 12 hsa-miR-365 0.31 down 13 hsa-miR-423-5p 0.35 down 14 hsa-miR-483-5p 0.25 down 15 hsa-miR-634 0.31 down 16 hsa-miR-671-5p 0.23 down 17 hsa-miR-939 0.24 down 18 hsa-miR-1246 2.22 up 19 hsa-miR-150 10.41 up 20 hsa-miR-574-5p 8.04 up Table 3 MiRNAs Target Gene Symbol hsa-miR-345PUM2, PPP2R3A, BCAT1, ZFHX4, CHSY3, ARNT, SHE, SLC7A5, SOS1,. [score:5]
control Expression in case group 1 hsa-mir-1290 0.26 down 2 hsa-mir-342-5p 0.22 down 3 hsa-mir-1224-5p 0.23 down 4 hsa-mir-345 0.38 down 5 hsa-mir-1228 0.38 down 6 hsa-mir-1249 0.32 down 7 hsa-mir-1826 0.26 down 8 hsa-miR-1306 0.38 down 9 hsa-miR-188-5p 0.43 down 10 hsa-miR-320a 0.48 down 11 hsa-miR-320c 0.26 down 12 hsa-miR-365 0.31 down 13 hsa-miR-423-5p 0.35 down 14 hsa-miR-483-5p 0.25 down 15 hsa-miR-634 0.31 down 16 hsa-miR-671-5p 0.23 down 17 hsa-miR-939 0.24 down 18 hsa-miR-1246 2.22 up 19 hsa-miR-150 10.41 up 20 hsa-miR-574-5p 8.04 up Table 3 MiRNAs Target Gene Symbol hsa-miR-345PUM2, PPP2R3A, BCAT1, ZFHX4, CHSY3, ARNT, SHE, SLC7A5, SOS1,. [score:5]
[1 to 20 of 2 sentences]
5
[+] score: 8
With an exceedingly stringent Sidak correction, only 4 differentially regulated miRs were identified; miR-200a, 345, 376c, and 888. miR345 is a methylation-sensitive miR (down-regulated) which is involved in cell proliferation and invasion in colorectal cancer [47], while miR-376c (here up-regulated) enhances ovarian cancer cell survival and has been implicated in chemoresistance [48]. [score:8]
[1 to 20 of 1 sentences]
6
[+] score: 8