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8 publications mentioning dme-mir-286

Open access articles that are associated with the species Drosophila melanogaster and mention the gene name mir-286. Click the [+] symbols to view sentences that include the gene name, or the word cloud on the right for a summary.

1
[+] score: 27
Differentially expressed miRNAs were identified at the threshold [FDR < 0.05 and log [2](fold change) ≥|1.0|] of 91-C/ 91-R. [b] Fold change was calculated as 91-C/ 91-R. The RT-qPCR validation of the predicted significant quantitative differences in miRNA levels among eight known and four novel miRNAs were amplified showing that the expression levels of miR-986-5p were highly up-regulated in 91-R, whereas miR-286-3p, miR-4919-3p, miR-311-3p, miR-312-3p, and miR-313-3p were significantly down-regulated in 91-R (Fig 1). [score:9]
Differentially expressed miRNAs were identified at the threshold [FDR < 0.05 and log [2](fold change) ≥|1.0|] of 91-C/ 91-R. [b] Fold change was calculated as 91-C/ 91-R. The RT-qPCR validation of the predicted significant quantitative differences in miRNA levels among eight known and four novel miRNAs were amplified showing that the expression levels of miR-986-5p were highly up-regulated in 91-R, whereas miR-286-3p, miR-4919-3p, miR-311-3p, miR-312-3p, and miR-313-3p were significantly down-regulated in 91-R (Fig 1). [score:9]
Furthermore, other corresponding predicted detoxification targets, Esterase8, Esterase10, GstD1, GstE10, GstS1, GABA-R, mAChR-A, nAcRalpha-A and GluR-IB were significantly up-regulated with the down-regulation of miR-286-3p, miR-2a-3p, miR-311-3p, miR-312-3p, and miR-313-3p in 91-R strain (Fig 4). [score:9]
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2
[+] score: 23
Mating causes a reduction in expression of mir-34, mir-92b and mir-988 and an increase in expression of mir-286 (Figure  3; Additional file 4). [score:5]
Females that overexpress mir-34 and mir-92b lay 34% and 37% fewer eggs after mating, whereas females that overexpress mir-286 and mir-988 lay 16% and 33% more eggs than controls (Figure  7). [score:5]
To test the connectivity of the computational networks (Figures  4, 5 and 6) and evaluate causality, we overexpressed six miRNAs for which overexpression lines were available (mir-281-1, mir-286, mir-34, mir-92b, mir-310 and mir-988) using the binary GAL4-UAS expression system [38] under a universal ubiquitin promoter. [score:5]
To assess causality between post-mating modulation of miRNA expression and oviposition, we measured egg laying after mating by control females and females overexpressing mir-286, mir-34, mir-92b, or mir-988. [score:3]
Number of eggs laid by five young mated females over 18 hours between control and mir-286, mir-34, mir-92b and mir-988 overexpression lines. [score:3]
We obtained UAS-mir-281-1, UAS-mir-286, UAS-mir-34, UAS-mir-92b, UAS-mir-988, mir-310 and the co-isogenic control lines from the Bloomington Stock Center. [score:1]
Of particular interest is the miRNA 309 cluster, which, in addition to mir-309, comprises mir-286, mir-3, mir-4, mir-5 and mir-6. The latter gives rise to three alternative stem-loop configurations with similar conserved seed regions [31, 32]. [score:1]
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3
[+] score: 19
In contrast, the other groups comprised a large number of targets; a common group of levelled miRNAs (miR-286-3p and miR-5-5p) may target 394 genes involved in most of the cellular processes regulated by levelled miRNAs (figure 3 b; electronic supplementary material, S3). [score:6]
As qPCR experiments showed, the levels of pri-mir-6 and pri-mir-286 decreased after HS, apparently owing to downregulation of the entire 6–309 cluster. [score:4]
However, mature miR-5-5p, miR-286-3p and miR-6-3p were concurrently upregulated after HS. [score:4]
Using qPCR, we found that HS exposure led to a decrease in the expression (two- to 2.5-fold, p ≤ 0.05) of several selected pri-miRNAs, specifically, pri-mir-14, pri-mir-6, pri-mir-286, pri-mir-311 and pri-mir-308 in the w [1118] strain (figure 5 a). [score:3]
The same applies to miR-6 and miR-286, which are encoded in the genome as miRNA cluster 6–309 and transcribed as a single pri-miRNA transcript. [score:1]
After a 24 h recovery period, pri-mir-14, pri-mir-6 and pri-mir-286 gradually returned to their original levels. [score:1]
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4
[+] score: 9
Strikingly, out of nine computationally predicted targets that were experimentally assayed but did not show any repression activity (likely false positives) [12], we only predicted one microRNA–target regulatory relationship (miR-286/boss). [score:5]
The highest scoring gene from all single microRNA target site predictions was nerfin-1, with two anchor sites for miR-286 conserved in all flies and many additional, non-aligned sites present in all flies. [score:3]
The only gene with a higher score (40.5) is nerfin-1, which contains two anchor sites for miR-286 (or equivalently miR-279) conserved in all flies, and many additional sites for the same microRNA (see ). [score:1]
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5
[+] score: 4
Finally, of the four conserved genomic miRNA clusters identified in B. dorsalis, only the mir-309-6 cluster (dme-mir-4, dme-mir-6-3, dme-mir-5, dme-mir-286, and dme-mir-309) had an analogous expression pattern across life stages. [score:3]
Six out of the 11 novel B. dorsalis miRNAs belong to this group, as did miRNAs belonging to the D. melanogaster miR-309-6 cluster (i. e. mir-4, mir-5 and mir-286), (Fig.   2, group 3). [score:1]
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6
[+] score: 4
Other miRNAs from this paper: dme-mir-279, dme-let-7, dme-mir-996
The mature sequences of three members of the miR-279 seed family are shown; note that miR-286 is encoded elsewhere in the genome but is more related to miR-279 than is miR-996. [score:1]
Nevertheless, mature miR-279 is more similar to miR-286 than it is to miR-996 (Fig 1A). [score:1]
A third seed member, mir-286, is genomically unlinked from the mir-279/996 cluster and moreover deployed in a spatially and temporally distinct manner, being essentially restricted to early embryogenesis [26, 29, 30] (S1 Fig). [score:1]
The accumulation of miR-286 is largely restricted to the early to mid embryo stages, and is nearly absent thereafter. [score:1]
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7
[+] score: 1
Similarly, “seed paralogs” were found for Drosophila mir-279, mir-285 and mir-286. [score:1]
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8
[+] score: 1
In the 15 missed reference pre-miRNAs, 4 did not pass the pre-processing filter due to their predicted double-loop structures (mir-2c, mir-31a, mir-31b, mir-286) and another 2 due to their low predicted free energy (mir-309, mir-311). [score:1]
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