sort by

15 publications mentioning dme-mir-276a

Open access articles that are associated with the species Drosophila melanogaster and mention the gene name mir-276a. Click the [+] symbols to view sentences that include the gene name, or the word cloud on the right for a summary.

[+] score: 47
In the case of miR-252, the-3p strand is expressed at much lower levels than those of the-5p strand, whereas in the case of miR-276, the situation is the reverse; therefore, in each case the pattern of expression of one strand is clearly different to the expression of the other (Figure  2). [score:7]
For expression pattern studies and hormonal treatments, we selected the three miRNAs more highly expressed (M-value between 1.6 and 5.3) in the N5 library (miR-252-3p, miR-276-5p and miR-190-5p) and the four more differentially expressed (M-value between −1.4 and −2.0) in the N6 library (bantam-3p, miR-100-5p, miR-125-5p and let-7-5p). [score:7]
In this case, however, the 3’ strand (miR-276-3p) is the predominant one in both libraries, being clearly more highly expressed in N6 with respect to N5, whereas the 5’ strand (miR-276-5p) is more highly expressed in the N5 with respect to N6. [score:5]
In support of this notion, Liu et al. [35] reported that both strands of miR-276 are differentially expressed in different tissues and stages of B. mori, although miR-276-3p is always the most highly expressed strand. [score:5]
Minor discrepancies, like in the case of miR-276-5p, miR-276-3p, miR-190-5p, can be due to individual variability, given that sequencing data come from a pool of nine specimens, whereas data of expression patterns is based on three particular specimens. [score:3]
These data do not correlate with quantitative sequencing data, which predicted that miR-276-5p and miR-190-5p should have shown much higher expression levels around the 20E peak of N5, whereas those of miR-252-5p and bantam-3p should have been much lower at this stage. [score:3]
Two expression bursts of miR-1-3p, miR-34-5p and mir-276-3p occurred in N6, the first just after emergence and the other coinciding or close to the peak of 20E of this stage (Figure  2). [score:3]
We also added three miRNAs that are similarly represented in N5 and N6: miR-252-5p and miR-276-3p, whose respective partner strands were chosen due to their higher expression in the N5 library, and miR-1-3p, which was the most abundant miRNA (accounting for more than 50% of total reads). [score:3]
However, the expression levels of miR-1-3p and miR-100-5p were significantly increased after treatment with 20E, whereas those of bantam-3p, miR-125-5p, miR-14-3p, miR-276-3p, miR-34-5p and let-7-5p showed a tendency to increase with respect to controls, although differences were not statistically significant. [score:3]
In the cases of miR-276-5p, miR-190-5p, miR-252-5p and bantam-3p, there were expression bursts seen both in N5 and in N6, approximately corresponding to the peaks of 20E (Figure  2). [score:3]
Of note, among the miRNAs selected to study the expression pattern, we included miR-252 and miR-276, which were represented by both the-3p and-5p strands. [score:3]
miR-276 represents a similar case, with both strands represented in both libraries. [score:1]
Some of the miRNAs (miR-8, miR-9a, miR-10, miR-71, miR-252, miR-276, miR-281) are represented by both strands,-3p and-5p, as was also observed in the previous N6 library [14]. [score:1]
[1 to 20 of 13 sentences]
[+] score: 16
Overexpression of non-mammalian (drosophila, C. elegans) and endogenous mouse miRNAsIt was previously reported that miRNAs derived from C. elegans or other lower species are difficult to express efficiently in mammalian cells in vitro 1. We therefore sought to compare the expression efficiency of endogenous mouse miRNAs (mir-107, mir-122, mir-675) and exogenous miRNAs from two non-mammalian species, namely drosophila (mir-14 and mir-276), and C. elegans (mir-77, mir-230). [score:7]
It was previously reported that miRNAs derived from C. elegans or other lower species are difficult to express efficiently in mammalian cells in vitro 1. We therefore sought to compare the expression efficiency of endogenous mouse miRNAs (mir-107, mir-122, mir-675) and exogenous miRNAs from two non-mammalian species, namely drosophila (mir-14 and mir-276), and C. elegans (mir-77, mir-230). [score:5]
We were able to recapitulate the lower expression level of C. elegans (miR-77-3p and miR-230-3p) miRNA and, to a lesser extent, of drosophila miRNA (miR-14-3p and miR-276a-3p), using reverse pri-mir or pre-mir strategy. [score:3]
This included the drosophila miR-14 (n = 5–9) and miR-276a (n = 5–15), the C. elegans miR-77 (n = 5–12) and miR-230 (n = 6–10), and mouse miR-107-3p (n = 6–15), miR-122-5p (n = 3–12), and miR-675-3p (n = 6–11). [score:1]
[1 to 20 of 4 sentences]
[+] score: 11
Mir-276a, had an equivalent trend in expression from embryo to pupa; however, the high expression of this miRNA in D. melanogaster ovary SSC was not observed in the fertilized B. dorsalis ovary library. [score:5]
[¥]Maximum count per million readsVisually inspecting each of the miRNA trend plots, there were 12 miRNAs with nearly identical expression patterns, and an additional miRNA (mir-276a), with a pattern varying only in the libraries derived from ovary tissue, which were the libraries with lowest correlation between the species. [score:3]
[¥]Maximum count per million reads Visually inspecting each of the miRNA trend plots, there were 12 miRNAs with nearly identical expression patterns, and an additional miRNA (mir-276a), with a pattern varying only in the libraries derived from ovary tissue, which were the libraries with lowest correlation between the species. [score:3]
[1 to 20 of 3 sentences]
[+] score: 11
Our analyses in the very early stages of embryo development (NFE and ED0) of B. germanica, T. castaneum, D. melanogaster and D. virilis indicated that miRNAs from MIR-276 and MIR-279 families are differentially expressed in short germ-band species, whereas those of MIR-92 family are differentially expressed in long germ-band species. [score:6]
The only reported function for MIR-276 is to promote egg-hatching synchrony by upregulating the transcription coactivator gene brahma in the locust Locusta migratoria [36]. [score:4]
PCA (Fig. 5b) showed this grouping to be mostly driven by the MIR-276, MIR-279 and MIR-92 families. [score:1]
[1 to 20 of 3 sentences]
[+] score: 5
Our data shows that MiR-276 was the most highly expressed miRNA in both morphs, wherein it represented 473,103 reads in the winged adults and 369,649 reads in the wingless adults. [score:3]
To date, miR-276 has been identified in over 34 organisms and may have a critical role in development among various organisms, but the function of this miRNA in insects remains unknown 40. [score:2]
[1 to 20 of 2 sentences]
[+] score: 4
bmo-miR-1, bmo-let-7a, bmo-miR-8, bmo-miR-14, bmo-miR-276a, bmo-miR-279 were strongly expressed in all developmental stages (larva, pupa and moth). [score:4]
[1 to 20 of 1 sentences]
[+] score: 4
The other case is mir-276a/b. [score:1]
The most likely explanation is that mir-276a/b in Daphnia resulted from an independent, lineage-specific, gene duplication. [score:1]
Not clustered in other insects Duplication mir-276a/bClustered together in Drosophila lineage >10 kb in D. melanogaster Duplication mir-285 Tandem duplication in vertebrates Duplication mir-285Clustered with mir-3556 and mir-3587 in R. norvegicus New hairpin [a]As annotated in miRBase (http://mirbase. [score:1]
We have found two instances of microRNA clusters in animals whose individual microRNAs are apparently not clustered in Drosophila (mir-1/mir-133 and mir-276a/mir-276b; Table 2). [score:1]
[1 to 20 of 4 sentences]
[+] score: 3
Four microRNA families were affected by multiple matching reads: mir-983, mir-281, mir-276 and mir-2. The first three did not show any differential expression between sexes. [score:3]
[1 to 20 of 1 sentences]
[+] score: 3
Other miRNAs from this paper: hsa-mir-33a, hsa-mir-92a-1, hsa-mir-92a-2, dme-mir-1, dme-mir-8, dme-mir-11, hsa-mir-34a, hsa-mir-210, dme-mir-184, dme-mir-275, dme-mir-92a, dme-mir-277, dme-mir-33, dme-mir-281-1, dme-mir-281-2, dme-mir-34, dme-mir-276b, dme-mir-210, dme-mir-92b, dme-bantam, dme-mir-309, dme-mir-317, hsa-mir-1-2, hsa-mir-184, hsa-mir-190a, hsa-mir-1-1, hsa-mir-34b, hsa-mir-34c, aga-bantam, aga-mir-1, aga-mir-184, aga-mir-210, aga-mir-275, aga-mir-276, aga-mir-277, aga-mir-281, aga-mir-317, aga-mir-8, aga-mir-92a, aga-mir-92b, hsa-mir-92b, hsa-mir-33b, hsa-mir-190b, dme-mir-190, dme-mir-957, dme-mir-970, dme-mir-980, dme-mir-981, dme-mir-927, dme-mir-989, dme-mir-252, dme-mir-1000, aga-mir-1174, aga-mir-1175, aga-mir-34, aga-mir-989, aga-mir-11, aga-mir-981, aga-mir-1889, aga-mir-1890, aga-mir-1891, aga-mir-190, aga-mir-927, aga-mir-970, aga-mir-957, aga-mir-1000, aga-mir-309, cqu-mir-1174, cqu-mir-281-1, cqu-mir-1, cqu-mir-275, cqu-mir-957, cqu-mir-277, cqu-mir-252-1, cqu-mir-970, cqu-mir-317-1, cqu-mir-981, cqu-mir-989, cqu-mir-1175, cqu-mir-276-1, cqu-mir-276-2, cqu-mir-276-3, cqu-mir-210, cqu-mir-92, cqu-mir-190-2, cqu-mir-190-1, cqu-mir-1000, cqu-mir-11, cqu-mir-8, cqu-bantam, cqu-mir-1891, cqu-mir-184, cqu-mir-1890, cqu-mir-980, cqu-mir-33, cqu-mir-2951, cqu-mir-2941-1, cqu-mir-2941-2, cqu-mir-2952, cqu-mir-1889, cqu-mir-309, cqu-mir-252-2, cqu-mir-281-2, cqu-mir-317-2, aga-mir-2944a-1, aga-mir-2944a-2, aga-mir-2944b, aga-mir-2945, aga-mir-33, aga-mir-980
Five miRNAs, miR-184, miR-275, miR-277, miR-276, and miR-92, were sequenced >500 times and were readily detectable in total RNA isolated from C7/10 cells (Figure 3A). [score:1]
quinquefasciatus miRNAs, miR-317, miR-252, miR-276, miR-190, miR-981, and miR-2944, arise from at least two possible hairpin precursors (Table 2). [score:1]
In Aedes, four miRNAs, miR-276, miR-317, miR-1000, and miR-309 arise from two potential hairpin precursors (Table 1). [score:1]
[1 to 20 of 3 sentences]
[+] score: 2
Importantly, levels of several miRNAs, including miR-33, miR-34, miR-276a, miR-317, miR-2b, miR-184 and miR-bantam, were significantly reduced upon SmD1 knockdown (Fig 1B–1G), reminiscent of the phenotype elicited by the loss of the canonical miRNA biogenesis enzyme Drosha. [score:2]
[1 to 20 of 1 sentences]
[+] score: 2
P. tepidariorum also contains panarthropod (mir-276 and mir-305), arthropod (iab-4/8 and mir-275), and chelicerate-specific (mir-3931) microRNAs (Rota-Stabelli et al. 2011; Tarver et al. 2013). [score:1]
Comparing P. tepidariorum with D. melanogaster, showed that mir-993b-2, both copies of mir-276, and again mir-2b-1 exhibited arm switching (fig. 4 B). [score:1]
[1 to 20 of 2 sentences]
[+] score: 1
elegans, Drosophila,Mouse, Humans miR-279 upd Rhythmicity N Insects miR-276a timeless Rhythmicity Peak at ZT10 Trough at ZT18 Insects miR-124 – Phase Peak at ZT19 Trough at ZT7C. [score:1]
[1 to 20 of 1 sentences]
[+] score: 1
In both strains, miR-184-3p miRNA was the most abundant among the means of non-normalized reads across triplicate libraries, with miR-8-3p, miR-276a-3p, bantam-3p, and miR-33-5p as the next most abundant miRNAs in both strains (Table 2). [score:1]
[1 to 20 of 1 sentences]
[+] score: 1
Other miRNAs from this paper: dme-mir-2a-1, dme-mir-2a-2, dme-mir-2b-1, dme-mir-2b-2, dme-mir-9a, dme-mir-10, dme-mir-12, dme-mir-13a, dme-mir-13b-1, dme-mir-13b-2, dme-mir-133, dme-mir-276b, dme-mir-210, dme-mir-31b, dme-mir-9c, dme-mir-306, dme-mir-9b, dme-mir-31a, dme-mir-309, dme-mir-316, dme-mir-317, dme-mir-2c, ame-mir-12, ame-mir-133, ame-mir-210, ame-mir-276, ame-mir-2-1, ame-mir-2-2, ame-mir-317, ame-mir-9a, ame-mir-9b, bmo-mir-9a, bmo-mir-10, bmo-mir-276, bmo-mir-31, bmo-mir-71, ame-mir-10, ame-mir-137, ame-mir-13a, ame-mir-2-3, ame-mir-29b, ame-mir-31a, ame-mir-375, ame-mir-71, ame-mir-932, dme-mir-193, dme-mir-375, dme-mir-932, dme-mir-970, dme-mir-971, dme-mir-989, dme-mir-137, dme-mir-1006, dme-mir-1007, bmo-mir-2a-1, bmo-mir-2a-2, bmo-mir-2b, bmo-mir-13a, bmo-mir-13b, bmo-mir-133, bmo-mir-210, bmo-mir-317, tca-mir-2-3, tca-mir-2-1, tca-mir-2-2, tca-mir-10, tca-mir-12, tca-mir-13a, tca-mir-13b, tca-mir-31, tca-mir-71, tca-mir-133, tca-mir-137, tca-mir-210, tca-mir-276, tca-mir-317, tca-mir-932, tca-mir-9b, bmo-mir-12, bmo-mir-137, bmo-mir-932, bmo-mir-9b, tca-mir-9a, tca-mir-970, ame-mir-13b, ame-mir-1006, ame-mir-316, bmo-mir-970, lmi-mir-276, lmi-mir-210, lmi-mir-10, lmi-mir-9a, bmo-mir-9c, bmo-mir-306a, bmo-mir-989a, bmo-mir-316, bmo-mir-1175, bmo-mir-9d, bmo-mir-750, bmo-mir-375, bmo-mir-306b, api-mir-137, api-mir-10, api-mir-276, api-mir-13a, api-mir-210, api-mir-29, api-mir-2a, api-mir-2b, api-mir-2c, api-mir-316, api-mir-317, api-mir-71, api-mir-971, api-mir-9a, api-mir-9b, api-mir-306, api-mir-3049, bmo-mir-989b, ame-mir-1175, ame-mir-193, ame-mir-989, ame-mir-3049, ame-mir-971, ame-mir-3770, ame-mir-9c, ame-mir-306, ame-mir-750, tca-mir-9c, tca-mir-316, tca-mir-9d, tca-mir-309a, tca-mir-3049, tca-mir-375, tca-mir-29, tca-mir-1175, tca-mir-750, tca-mir-989, tca-mir-309b, tca-mir-193, tca-mir-6012, tca-mir-9e, ame-mir-6037, ame-mir-6012, ame-mir-2b, tca-mir-309c, tca-mir-971b
With this approach, we discarded three miRNA loci that would correspond to MIR-137, MIR-29 and MIR-276 families, as they resulted from misassembling artefacts. [score:1]
[1 to 20 of 1 sentences]
[+] score: 1
The SLS output contained five predictions with significant similarity to the HOM output (ame-mir-13a, ame-mir-276, ame-mir-305, ame-mir-92 and ame-mir-9a) and only two predictions with significant similarity to the top 25 MCE candidates, both of which were variants of C5152. [score:1]
[1 to 20 of 1 sentences]