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5 publications mentioning cel-mir-47

Open access articles that are associated with the species Caenorhabditis elegans and mention the gene name mir-47. Click the [+] symbols to view sentences that include the gene name, or the word cloud on the right for a summary.

1
[+] score: 14
Through this method of data analysis, we found that miR-784, miR-231, miR-86, miR-53, miR-47, and miR-34 are normally upregulated by HSF-1 during HS. [score:4]
mir-47 is expressed constitutively throughout development, however the precise function of miR-47 is unknown. [score:4]
Induction of miR-784, miR-231, miR-86, miR-53, miR-47, and miR-34 by HSF-1 during HS is predicted to have the largest impact on the suppression of genes encoding proteins involved in the regulation of transcription and behavior, as these processes have enrichment scores of 5.49 and 4.93, respectively (S7A Fig). [score:4]
While the functions of miR-784, miR-231, miR-86, miR-53, and miR-47 are currently unknown, miR-34 encodes a microRNA that is highly conserved with orthologues in Drosophila, mouse, and human [30]. [score:1]
miR-47 0.22 miR-47 is conserved in C. briggsae. [score:1]
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2
[+] score: 13
Notably, we found mir-2-5p, mir-46-3p and mir-47-3p to be stably expressed in all conditions (Figures 2 and 3 and Additional file 4: Figure S1, Additional file 5: Figure S2, Additional file 6: Figure S3). [score:3]
All conditions: mir-47-3p (21043,167 reads), mir-46-3p (22186,815 reads) and mir-2-5p (5977,944 reads) were stably expressed in all the conditions and therefore chosen to validate by qPCR (Figure 4). [score:3]
In fact, a previous sequencing study has shown that two of the miRNAs identified in our study, mir-46-3p and mir-47-3p, are remarkably stable when expression levels are compared between young adults and aged animals [11]. [score:2]
I. mir-46-3p, mir-47-3p and mir-2-5p are stable in all conditions tested, II. [score:1]
Click here for file Graphical output files from mirDeep2 showing the reads, counts per read and mapping on the hairpin for mir-2, mir-46 and mir-47. [score:1]
Click here for file miRNAs included in Venn’s diagram of Figure 1. Click here for file Graphical output files from mirDeep2 showing the reads, counts per read and mapping on the hairpin for mir-2, mir-46 and mir-47. [score:1]
Graphical output files from mirDeep2 showing the reads, counts per read and mapping on the hairpin for mir-2, mir-46 and mir-47. [score:1]
Interestingly, we also found that 3 miRNAs, namely mir-2-5p, mir-46-3p and mir-47-3p, are stable in all the above-mentioned conditions suggesting that they might have general functions independent of stress. [score:1]
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3
[+] score: 11
We queried the database with all the miRNAs that were upregulated in young-adult eat-2(ad1116) worms collected on day 1. We found that 65 of the 82 promoters of upregulated miRNA have one or more PHA-4 binding peaks (Table 1), potentially pointing at direct transcriptional regulation of these miRNA by PHA-4. In order to validate this data, we chose 9 of these miRNAs that are induced in eat-2(ad1116) as compared to WT and checked their expression in eat-2(ad1116) mutants, both in presence or absence of PHA-4. We found that all these miRNAs, except cel-mir-47, are regulated in a PHA-4 -dependent manner (Figure 4A). [score:11]
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4
[+] score: 3
Among the dysregulated miRNAs, the further bioinformatics analysis demonstrated that lin-4, mir-228, mir-249, mir-47, mir-355, mir-45, mir-2210, mir-57, mir-1018, mir-360, mir-64, mir-2209, mit-793, mir-1830, mir-2210, mir-83, mir-789, and mir-806 might be involved in the control of MWCNTs toxicity through affecting the functions of identified dysregulated genes in exposed nematodes (Table S6). [score:3]
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5
[+] score: 1
The normalization of miRNA real-time PCRs was performed with geNorm (38) and NormFinder (39) algorithms, whereby the two most stable miRNAs across samples were miR-47 and miR-81 for TGF-β Sma/Mab genes, and miR-81 and miR-82 for the TGF-β Dauer pathway. [score:1]
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