Stem-loop sequence mmu-mir-329

Accession MI0000605
Symbol MGI:Mir329
Description Mus musculus miR-329 stem-loop
Gene family MIPF0000110; mir-329
Stem-loop 
uguucgcuuc      c            uu      cuc      -   g 
          ugguac ggaagagagguu  cugggu   uguuuc uuu a
          |||||| ||||||||||||  ||||||   |||||| ||| u
          acuaug cuuuuuuuccaa  gaccca   acaaag aag g
-----ccuaa      a            uc      --c      u   a
Get sequence
Deep sequencing
12065 reads, 62 experiments
Comments

Kim et al. cloned 40 new miRNAs from rat E18 primary cortical neurons [1]. This mouse miRNA is predicted based on homology to the verified rat sequence (MI0000604 ). Seitz et al. independently predicted the miRNA hairpin precursor sequence by conservation between mouse and human [2]. Landgraf et al. verified expression by cloining [3].

Genome context
Coordinates (GRCm38) Overlapping transcripts
chr12: 109713481-109713577 [+]
intergenic
Clustered miRNAs
< 10kb from mmu-mir-329
mmu-mir-379 chr12: 109709060-109709125 [+]
mmu-mir-411 chr12: 109710175-109710256 [+]
mmu-mir-299a chr12: 109710638-109710700 [+]
mmu-mir-299b chr12: 109710645-109710693 [-]
mmu-mir-380 chr12: 109711803-109711863 [+]
mmu-mir-1197 chr12: 109712317-109712436 [+]
mmu-mir-323 chr12: 109712508-109712593 [+]
mmu-mir-758 chr12: 109712810-109712890 [+]
mmu-mir-329 chr12: 109713481-109713577 [+]
mmu-mir-494 chr12: 109715318-109715402 [+]
mmu-mir-679 chr12: 109715577-109715650 [+]
mmu-mir-1193 chr12: 109715671-109715791 [+]
mmu-mir-666 chr12: 109717085-109717183 [+]
mmu-mir-543 chr12: 109717258-109717333 [+]
mmu-mir-495 chr12: 109718754-109718816 [+]
mmu-mir-667 chr12: 109720006-109720097 [+]
mmu-mir-376c chr12: 109722718-109722803 [+]
mmu-mir-654 chr12: 109723218-109723301 [+]
mmu-mir-376b chr12: 109723458-109723539 [+]
Database links

Mature sequence mmu-miR-329-5p

Accession MIMAT0017032
Previous IDs mmu-miR-329*
Sequence

23 -  

agagguuuucugggucucuguu

 - 44

Get sequence
Deep sequencing 8017 reads, 57 experiments
Evidence experimental; 454 [5], Solexa [6]
Predicted targets

Mature sequence mmu-miR-329-3p

Accession MIMAT0000567
Previous IDs mmu-miR-329
Sequence

61 -  

aacacacccagcuaaccuuuuu

 - 82

Get sequence
Deep sequencing 4046 reads, 37 experiments
Evidence experimental; cloned [3], Solexa [4,6]
Predicted targets

References

1
PMID:14691248 "Identification of many microRNAs that copurify with polyribosomes in mammalian neurons" Kim J, Krichevsky A, Grad Y, Hayes GD, Kosik KS, Church GM, Ruvkun G Proc Natl Acad Sci U S A. 101:360-365(2004).
2
PMID:15310658 "A large imprinted microRNA gene cluster at the mouse Dlk1-Gtl2 domain" Seitz H, Royo H, Bortolin ML, Lin SP, Ferguson-Smith AC, Cavaille J Genome Res. 14:1741-1748(2004).
3
PMID:17604727 "A mammalian microRNA expression atlas based on small RNA library sequencing" Landgraf P, Rusu M, Sheridan R, Sewer A, Iovino N, Aravin A, Pfeffer S, Rice A, Kamphorst AO, Landthaler M, Lin C, Socci ND, Hermida L, Fulci V, Chiaretti S, Foa R, Schliwka J, Fuchs U, Novosel A, Muller RU, Schermer B, Bissels U, Inman J, Phan Q, Chien M Cell. 129:1401-1414(2007).
4
PMID:20215419 "MicroRNA transcriptome in the newborn mouse ovaries determined by massive parallel sequencing" Ahn HW, Morin RD, Zhao H, Harris RA, Coarfa C, Chen ZJ, Milosavljevic A, Marra MA, Rajkovic A Mol Hum Reprod. 16:463-471(2010).
5
PMID:20668074 "Identification and analysis of expression of novel microRNAs of murine gammaherpesvirus 68" Zhu JY, Strehle M, Frohn A, Kremmer E, Hofig KP, Meister G, Adler H J Virol. 84:10266-10275(2010).
6
PMID:20413612 "Mammalian microRNAs: experimental evaluation of novel and previously annotated genes" Chiang HR, Schoenfeld LW, Ruby JG, Auyeung VC, Spies N, Baek D, Johnston WK, Russ C, Luo S, Babiarz JE, Blelloch R, Schroth GP, Nusbaum C, Bartel DP Genes Dev. 24:992-1009(2010).