Stem-loop sequence mmu-mir-199a-1
|Description||Mus musculus miR-199a-1 stem-loop|
|Gene family||MIPF0000040; mir-199|
This text is a summary paragraph taken from the Wikipedia entry entitled mir-199_microRNA_precursor. miRBase and Rfam are facilitating community annotation of microRNA families and entries in Wikipedia. Read more ...
The miR-199 microRNA precursor (homologous to miR-215), is a short non-coding RNA gene involved in gene regulation. miR-192 and miR-215 have now been predicted or experimentally confirmed in mouse, human and a further 21 other species. (MIPF0000040). microRNAs are transcribed as ~70 nucleotide precursors and subsequently processed by the Dicer enzyme to give a ~22 nucleotide product. The mature products are thought to have regulatory roles through complementarity to mRNA.
The miR-199 microRNA precursor (homologous to miR-215), is a short non-coding RNA gene involved in gene regulation. miR-192 and miR-215 have now been predicted or experimentally confirmed in mouse, human and a further 21 other species.    (MIPF0000040). microRNAs are transcribed as ~70 nucleotide precursors and subsequently processed by the Dicer enzyme to give a ~22 nucleotide product. The mature products are thought to have regulatory roles through complementarity to mRNA.
 Targets of miR-199
MicroRNA hsa-miR-199a is a regulator of IκB kinase-β (IKKβ) expression.
 Expression patterns of miR-199
Using TaqMan real-time quantitative PCR array methods, miRNA expression has been profiled. miR-199a, one of the most significantly overexpressed in invasive squamous cell carcinomas (ISCCs), was evaluated by transfecting cervical cancer cells (SiHa and ME-180) with anti-miR-199a oligonucleotides and the cell viability assessed. mirR-199a*, mir199a and mirR-199b were significantly overexpressed in ISCCs.
Comparative miRNA array led to the isolation of several Bone Morphogenic Protein 2 (BMP2)-responsive miRNAs. Among them, miR-199a* is of particular interest, because it was reported to be specifically expressed in the skeletal system.
 Clinical relevance of miR-199
Alcoholic liver disease is a common medical consequence of chronic alcohol abuse. Activation of hypoxia-Inducible Factor-1α (HIF-1α) is an indicator of hypoxia. Endothelin-1 (ET-1) is a protein that constricts blood vessels and raises blood pressure. It has been shown that ethanol-induced miR-199 down-regulation may contribute to augmented HIF-1α and ET-1 expression.
 Further reading
auc u c ------- g gcc ccagugu cagacuac ugu ucag a ||| ||||||| |||||||| ||| |||| cgg gguuaca gucugaug aca gguc g auu c - uguacag g
The hairpin precursor sequence mir-199 maps to two loci within 50 kb on mouse chromosome 9. This sequence was named mir-199 in  and , but is renamed here to avoid overlap with the predicted homologues of human mir-199a-2 and mir-199b. Landgraf et al. demonstrate comparable expression for products from both arms of the hairpin, which are therefore renamed miR-199a-5p and miR-199a-3p here . The mature sequences shown here represent the most commonly cloned forms from large-scale cloning studies . The 5' end of the miRNA may be offset with respect to previous annotations.
Mature sequence mmu-miR-199a-5p
|Deep sequencing||188578 reads, 67 experiments|
|Evidence||experimental; cloned [2,4], Northern , Solexa [5-6]|
Mature sequence mmu-miR-199a-3p
|Deep sequencing||2125160 reads, 82 experiments|
|Evidence||experimental; cloned [1-4], Northern , Solexa [5-6]|
PMID:12554860 "Numerous microRNPs in neuronal cells containing novel microRNAs" RNA. 9:180-186(2003).
PMID:17604727 "A mammalian microRNA expression atlas based on small RNA library sequencing" Cell. 129:1401-1414(2007).
PMID:20215419 "MicroRNA transcriptome in the newborn mouse ovaries determined by massive parallel sequencing" Mol Hum Reprod. 16:463-471(2010).
PMID:20413612 "Mammalian microRNAs: experimental evaluation of novel and previously annotated genes" Genes Dev. 24:992-1009(2010).