Stem-loop sequence mmu-mir-99b

Accession

MI0000147 (change log)

Symbol

MGI:Mir99b

Description

Mus musculus miR-99b stem-loop

Gene family

MIPF0000033; mir-10

Literature search

54 open access papers mention mmu-mir-99b
(462 sentences)

Stem-loop

        cc         ac   c    c - -   c 
5' ggcac  acccguaga  cga cuug g g ggc u
   |||||  |||||||||  ||| |||| | | |||  
3' cugug  ugggugucu  gcu gaac c c ccg u
        cc         gu   c    a a g   c 

Deep sequencing

3508668 reads, 2.99e+03 reads per million, 109 experiments

Confidence

Annotation confidence: high

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Comments

Expression of this miRNA in mouse was independently verified in the brain [1], embryonic stem cells [2] and testes [3].

Genome context

• Coordinates (GRCm38; GCA_000001635.2): chr17: 17830188-17830257 [+]
• Overlapping transcripts:

  intergenic

Clustered miRNAs

< 10kb from mmu-mir-99b

• mmu-mir-99b: chr17: 17830188-17830257 [+]
• mmu-let-7e: chr17: 17830352-17830444 [+]
• mmu-mir-125a: chr17: 17830812-17830879 [+]

Database links

• EntrezGene:Mirn99b
• MGI:Mir99b
• RNAcentral:URS000075E904_10090
• miRBase Tracker:MI0000147

Mature sequence mmu-miR-99b-5p

• Accession: MIMAT0000132
• Previous IDs: mmu-miR-99b
• Sequence: 7 - cacccguagaaccgaccuugcg - 28
• Deep sequencing: 3489428 reads, 109 experiments
• Evidence: experimental; cloned [1-4], Illumina [5-6]
• Database links:
  • RNAcentral:URS00002C10B3_10090
• Predicted targets:
  • TargetScanVert:mmu-miR-99b-5p
  • miRDB:mmu-miR-99b-5p
  • microrna.org:mmu-miR-99b-5p

Mature sequence mmu-miR-99b-3p

• Accession: MIMAT0004525
• Previous IDs: mmu-miR-99b*
• Sequence: 45 - caagcucgugucuguggguccg - 66
• Deep sequencing: 19240 reads, 96 experiments
• Evidence: experimental; cloned [4], Illumina [5-6]
• Database links:
  • RNAcentral:URS00001C308D_10090
• Predicted targets:
  • TargetScanVert:mmu-miR-99b-3p
  • miRDB:mmu-miR-99b-3p
  • microrna.org:mmu-miR-99b-3p

References

1. PMID:12007417 "Identification of tissue-specific microRNAs from mouse" Lagos-Quintana M, Rauhut R, Yalcin A, Meyer J, Lendeckel W, Tuschl T Curr Biol. 12:735-739(2002).
2. PMID:12919684 "Embryonic stem cell-specific MicroRNAs" Houbaviy HB, Murray MF, Sharp PA Dev Cell. 5:351-358(2003).
3. PMID:16766679 "Identification and characterization of two novel classes of small RNAs in the mouse germline: retrotransposon-derived siRNAs in oocytes and germline small RNAs in testes" Watanabe T, Takeda A, Tsukiyama T, Mise K, Okuno T, Sasaki H, Minami N, Imai H Genes Dev. 20:1732-1743(2006).
4. PMID:17604727 "A mammalian microRNA expression atlas based on small RNA library sequencing" Landgraf P, Rusu M, Sheridan R, Sewer A, Iovino N, Aravin A, Pfeffer S, Rice A, Kamphorst AO, Landthaler M, Lin C, Socci ND, Hermida L, Fulci V, Chiaretti S, Foa R, Schliwka J, Fuchs U, Novosel A, Muller RU, Schermer B, Bissels U, Inman J, Phan Q, Chien M Cell. 129:1401-1414(2007).
5. PMID:20215419 "MicroRNA transcriptome in the newborn mouse ovaries determined by massive parallel sequencing" Ahn HW, Morin RD, Zhao H, Harris RA, Coarfa C, Chen ZJ, Milosavljevic A, Marra MA, Rajkovic A Mol Hum Reprod. 16:463-471(2010).
6. PMID:20413612 "Mammalian microRNAs: experimental evaluation of novel and previously annotated genes" Chiang HR, Schoenfeld LW, Ruby JG, Auyeung VC, Spies N, Baek D, Johnston WK, Russ C, Luo S, Babiarz JE, Blelloch R, Schroth GP, Nusbaum C, Bartel DP Genes Dev. 24:992-1009(2010).