Stem-loop sequence bna-MIR156b

AccessionMI0006481
DescriptionBrassica napus miR156b stem-loop
Gene family MIPF0000008; MIR156
Community annotation

This text is a summary paragraph taken from the Wikipedia entry entitled mir-156_microRNA_precursor. miRBase and Rfam are facilitating community annotation of microRNA families and entries in Wikipedia. Read more ...

MicroRNA (miRNA) precursor mir-156 is a family of plant non-coding RNA. This microRNA has now been predicted or experimentally confirmed in a range of plant species (MIPF0000008). Animal miRNAs are transcribed as ~70 nucleotide precursors and subsequently processed by the Dicer enzyme to give a ~22 nucleotide product. In plants the precursor sequences may be longer, and the carpel factory (caf) enzyme appears to be involved in processing. In this case the mature sequence comes from the 5' arm of the precursor, and both Arabidopsis thaliana and rice genomes contain a number of related miRNA precursors which give rise to almost identical mature sequences. The extents of the hairpin precursors are not generally known and are estimated based on hairpin prediction. The products are thought to have regulatory roles through complementarity to mRNA.

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Stem-loop
        uu     ------u    c    u  u        -           u     --u        a  a auauguauguaucaucacaccgccuguggaugau 
5' uaggu  gagag       gaug uggu gu gacagaag auagagagcac aagga   gacaugca gu c                                  u
   |||||  |||||       |||| |||| || |||||||| ||||||||||| |||||   |||||||| || |                                   
3' aucca  cuuuc       uuau acca ca cugucuuc uaucucucgug uuucu   cuguacgu cg g                                  a
        uu     uucuucu    u    c  -        a           c     cau        c  a agagagagagagaaaacuuaaccaaaauaaaaac 
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Confidence
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Genome context
Coordinates (BrassicaDB-20080411) Overlapping transcripts
EM:AC189250: 143274-143481 [-]
intergenic

Mature sequence bna-miR156b

Accession MIMAT0005636
Sequence

25 - 

uugacagaagauagagagcac

 - 45

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Evidence experimental; cloned [1]

References

1
PMID:17659282 "Cloning and characterization of microRNAs from Brassica napus" Wang L, Wang MB, Tu JX, Helliwell CA, Waterhouse PM, Dennis ES, Fu TD, Fan YL FEBS Lett. 581:3848-3856(2007).