Stem-loop sequence hsa-mir-543

Accession MI0005565
Symbol HGNC:MIR543
Description Homo sapiens miR-543 stem-loop
Gene family MIPF0000110; mir-329
Stem-loop 
--    u  u       u  -     ---u    u    uu 
  uacu aa gagaagu gc ccgug    uuuu ucgc  u
  |||| || ||||||| || |||||    |||| ||||  a
  auga uu uucuuca cg ggcgc    aaag agug  u
cu    c  u       -  u     uuac    c    uu
Get sequence
Deep sequencing
2115 reads, 10 experiments
Comments

This sequence was identified as a miRNA candidate by Berezikov et al. using RAKE and MPSS techniques [1]. Expression has been independently confirmed in mouse and rat [2].

Genome context
Coordinates (GRCh37.p5) Overlapping transcripts
chr14: 101498324-101498401 [+]
intergenic
Clustered miRNAs
< 10kb from hsa-mir-543
hsa-mir-379 chr14: 101488403-101488469 [+]
hsa-mir-411 chr14: 101489662-101489757 [+]
hsa-mir-299 chr14: 101490131-101490193 [+]
hsa-mir-380 chr14: 101491354-101491414 [+]
hsa-mir-1197 chr14: 101491901-101491988 [+]
hsa-mir-323a chr14: 101492069-101492154 [+]
hsa-mir-758 chr14: 101492357-101492444 [+]
hsa-mir-329-1 chr14: 101493122-101493201 [+]
hsa-mir-329-2 chr14: 101493437-101493520 [+]
hsa-mir-494 chr14: 101495971-101496051 [+]
hsa-mir-1193 chr14: 101496389-101496466 [+]
hsa-mir-543 chr14: 101498324-101498401 [+]
hsa-mir-495 chr14: 101500092-101500173 [+]
hsa-mir-376c chr14: 101506027-101506092 [+]
hsa-mir-376a-2 chr14: 101506406-101506485 [+]
hsa-mir-654 chr14: 101506556-101506636 [+]
hsa-mir-376b chr14: 101506773-101506872 [+]
hsa-mir-376a-1 chr14: 101507119-101507186 [+]
hsa-mir-300 chr14: 101507700-101507782 [+]
Database links

Mature sequence hsa-miR-543

Accession MIMAT0004954
Sequence

47 -  

aaacauucgcggugcacuucuu

 - 68

Get sequence
Deep sequencing 2111 reads, 7 experiments
Evidence experimental; RAKE [1]
Predicted targets

References

1
PMID:16954537 "Many novel mammalian microRNA candidates identified by extensive cloning and RAKE analysis" Berezikov E, van Tetering G, Verheul M, van de Belt J, van Laake L, Vos J, Verloop R, van de Wetering M, Guryev V, Takada S, van Zonneveld AJ, Mano H, Plasterk R, Cuppen E Genome Res. 16:1289-1298(2006).
2
PMID:17604727 "A mammalian microRNA expression atlas based on small RNA library sequencing" Landgraf P, Rusu M, Sheridan R, Sewer A, Iovino N, Aravin A, Pfeffer S, Rice A, Kamphorst AO, Landthaler M, Lin C, Socci ND, Hermida L, Fulci V, Chiaretti S, Foa R, Schliwka J, Fuchs U, Novosel A, Muller RU, Schermer B, Bissels U, Inman J, Phan Q, Chien M Cell. 129:1401-1414(2007).