Stem-loop sequence hsa-mir-449c

Accession

MI0003823

Description

Homo sapiens miR-449c stem-loop

Gene family

MIPF0000133; mir-449

Stem-loop

   --g       u   gu   c      uu               u 
gcu   ggaugug cag  agg agugua  gcuagcggcuguuaa g
|||   ||||||| |||  ||| ||||||  ||||||||||||||| a
cga   cuuacgu guc  ucc ucacgu  ugaucguugacaauu u
   aga       u   uc   -      --               u 

Deep sequencing

3 reads, 3 experiments

Comments

This sequence was identified as a miRNA candidate by Berezikov et al. using the RAKE techniques [1]. Expression was later confirmed by Wyman et al. [2].

Genome context

• Coordinates (GRCh37.p5): chr5: 54468090-54468181 [-]
• Overlapping transcripts:

  sense

  OTTHUMT00000369716 ; CDC20B-005; intron 1

  OTTHUMT00000369716 ; CDC20B-005; intron 1

  OTTHUMT00000369716 ; CDC20B-005; intron 1

  OTTHUMT00000253908 ; CDC20B-001; intron 2

  OTTHUMT00000369715 ; CDC20B-004; intron 2

  OTTHUMT00000369714 ; CDC20B-003; intron 2

  OTTHUMT00000369713 ; CDC20B-002; intron 2

  OTTHUMT00000253908 ; CDC20B-001; intron 2

  OTTHUMT00000369715 ; CDC20B-004; intron 2

  OTTHUMT00000369714 ; CDC20B-003; intron 2

  OTTHUMT00000369713 ; CDC20B-002; intron 2

  OTTHUMT00000253908 ; CDC20B-001; intron 2

  OTTHUMT00000369715 ; CDC20B-004; intron 2

  OTTHUMT00000369714 ; CDC20B-003; intron 2

  OTTHUMT00000369713 ; CDC20B-002; intron 2

  ENST00000507931 ; CDC20B-005; intron 1

  ENST00000331730 ; CDC20B-201; intron 1

  ENST00000507931 ; CDC20B-005; intron 1

  ENST00000331730 ; CDC20B-201; intron 1

  ENST00000507931 ; CDC20B-005; intron 1

  ENST00000331730 ; CDC20B-201; intron 1

  ENST00000296733 ; CDC20B-001; intron 2

  ENST00000381375 ; CDC20B-004; intron 2

  ENST00000322374 ; CDC20B-003; intron 2

  ENST00000513180 ; CDC20B-002; intron 2

  ENST00000334206 ; CDC20B-202; intron 2

  ENST00000296733 ; CDC20B-001; intron 2

  ENST00000381375 ; CDC20B-004; intron 2

  ENST00000322374 ; CDC20B-003; intron 2

  ENST00000513180 ; CDC20B-002; intron 2

  ENST00000334206 ; CDC20B-202; intron 2

  ENST00000296733 ; CDC20B-001; intron 2

  ENST00000381375 ; CDC20B-004; intron 2

  ENST00000322374 ; CDC20B-003; intron 2

  ENST00000513180 ; CDC20B-002; intron 2

  ENST00000334206 ; CDC20B-202; intron 2

Clustered miRNAs

< 10kb from hsa-mir-449c

• hsa-mir-449c: chr5: 54468090-54468181 [-]
• hsa-mir-449b: chr5: 54466474-54466570 [-]
• hsa-mir-449a: chr5: 54466360-54466450 [-]

Database links

• ENTREZGENE: 100313923;MIR449C

Mature sequence hsa-miR-449c-5p

• Accession: MIMAT0010251
• Previous IDs: hsa-miR-449c
• Sequence: 17 - uaggcaguguauugcuagcggcugu - 41
• Deep sequencing: 2 reads, 2 experiments
• Evidence: experimental; RAKE [1], 454 [2]
• Predicted targets:
  • DIANA-MICROT:hsa-miR-449c-5p
  • MICRORNA.ORG:hsa-miR-449c-5p
  • MIRDB:hsa-miR-449c-5p
  • RNA22-HSA:hsa-miR-449c-5p
  • TARGETMINER:hsa-miR-449c-5p

Mature sequence hsa-miR-449c-3p

• Accession: MIMAT0013771
• Previous IDs: hsa-miR-449c*
• Sequence: 57 - uugcuaguugcacuccucucugu - 79
• Evidence: experimental; 454 [2]
• Predicted targets:
  • DIANA-MICROT:hsa-miR-449c-3p
  • MICRORNA.ORG:hsa-miR-449c-3p
  • MIRDB:hsa-miR-449c-3p
  • RNA22-HSA:hsa-miR-449c-3p
  • TARGETMINER:hsa-miR-449c-3p

References

1. PMID:16954537 "Many novel mammalian microRNA candidates identified by extensive cloning and RAKE analysis" Berezikov E, van Tetering G, Verheul M, van de Belt J, van Laake L, Vos J, Verloop R, van de Wetering M, Guryev V, Takada S, van Zonneveld AJ, Mano H, Plasterk R, Cuppen E Genome Res. 16:1289-1298(2006).
2. PMID:19390579 "Repertoire of microRNAs in epithelial ovarian cancer as determined by next generation sequencing of small RNA cDNA libraries" Wyman SK, Parkin RK, Mitchell PS, Fritz BR, O'Briant K, Godwin AK, Urban N, Drescher CW, Knudsen BS, Tewari M PLoS One. 4:e5311(2009).