Stem-loop sequence hsa-mir-654

Accession

MI0003676

Symbol

HGNC:MIR654

Description

Homo sapiens miR-654 stem-loop

Gene family

MIPF0000409; mir-654

Stem-loop

    aagugg    a      g c     a      - -  a 
gggu      aaag uggugg c gcaga caugug c ug g
||||      |||| |||||| | ||||| |||||| | ||  
cccg      uuuc acuacc g cgucu guauac g gc u
    --aaga    c      a u     -      c u  u 

Deep sequencing

304 reads, 12 experiments

Genome context

• Coordinates (GRCh37.p5): chr14: 101506556-101506636 [+]
• Overlapping transcripts:

  intergenic

Clustered miRNAs

< 10kb from hsa-mir-654

• hsa-mir-543: chr14: 101498324-101498401 [+]
• hsa-mir-495: chr14: 101500092-101500173 [+]
• hsa-mir-376c: chr14: 101506027-101506092 [+]
• hsa-mir-376a-2: chr14: 101506406-101506485 [+]
• hsa-mir-654: chr14: 101506556-101506636 [+]
• hsa-mir-376b: chr14: 101506773-101506872 [+]
• hsa-mir-376a-1: chr14: 101507119-101507186 [+]
• hsa-mir-300: chr14: 101507700-101507782 [+]
• hsa-mir-1185-1: chr14: 101509314-101509399 [+]
• hsa-mir-1185-2: chr14: 101510535-101510620 [+]
• hsa-mir-381: chr14: 101512257-101512331 [+]
• hsa-mir-487b: chr14: 101512792-101512875 [+]
• hsa-mir-539: chr14: 101513658-101513735 [+]
• hsa-mir-889: chr14: 101514238-101514316 [+]
• hsa-mir-544a: chr14: 101514995-101515085 [+]
• hsa-mir-655: chr14: 101515887-101515983 [+]

Database links

• ENTREZGENE: 724024;MIR654
• HGNC: 32910;MIR654

Mature sequence hsa-miR-654-5p

• Accession: MIMAT0003330
• Previous IDs: hsa-miR-654
• Sequence: 16 - uggugggccgcagaacaugugc - 37
• Deep sequencing: 52 reads, 4 experiments
• Evidence: experimental; Microarray [1], SAGE [1], cloned [2]
• Predicted targets:
  • DIANA-MICROT:hsa-miR-654-5p
  • MICRORNA.ORG:hsa-miR-654-5p
  • MIRDB:hsa-miR-654-5p
  • RNA22-HSA:hsa-miR-654-5p
  • TARGETMINER:hsa-miR-654-5p
  • TARGETSCAN-VERT:hsa-miR-654-5p

Mature sequence hsa-miR-654-3p

• Accession: MIMAT0004814
• Sequence: 51 - uaugucugcugaccaucaccuu - 72
• Deep sequencing: 252 reads, 9 experiments
• Evidence: experimental; cloned [2]
• Validated targets:
  • MIRTARBASE:hsa-miR-654-3p
  • TARBASE:hsa-miR-654-3p
• Predicted targets:
  • DIANA-MICROT:hsa-miR-654-3p
  • MICRORNA.ORG:hsa-miR-654-3p
  • MIRDB:hsa-miR-654-3p
  • RNA22-HSA:hsa-miR-654-3p
  • TARGETMINER:hsa-miR-654-3p
  • TARGETSCAN-VERT:hsa-miR-654-3p

References

1. PMID:16505370 "The colorectal microRNAome" Cummins JM, He Y, Leary RJ, Pagliarini R, Diaz LA Jr, Sjoblom T, Barad O, Bentwich Z, Szafranska AE, Labourier E, Raymond CK, Roberts BS, Juhl H, Kinzler KW, Vogelstein B, Velculescu VE Proc Natl Acad Sci U S A. 103:3687-3692(2006).
2. PMID:17604727 "A mammalian microRNA expression atlas based on small RNA library sequencing" Landgraf P, Rusu M, Sheridan R, Sewer A, Iovino N, Aravin A, Pfeffer S, Rice A, Kamphorst AO, Landthaler M, Lin C, Socci ND, Hermida L, Fulci V, Chiaretti S, Foa R, Schliwka J, Fuchs U, Novosel A, Muller RU, Schermer B, Bissels U, Inman J, Phan Q, Chien M Cell. 129:1401-1414(2007).