Stem-loop sequence mmu-mir-494

Accession

MI0003532

Symbol

MGI:Mir494

Description

Mus musculus miR-494 stem-loop

Gene family

MIPF0000018; mir-154

Stem-loop

u       u            gu      -   c   -  uuu 
 ugauacu gaaggagagguu  ccgugu ugu uuc uc   a
 ||||||| ||||||||||||  |||||| ||| ||| ||   u
 acuauga uuuuuucuccaa  ggcaca aca aag ag   u
a       -            ag      u   -   u  uau 

Deep sequencing

16594 reads, 74 experiments

Comments

The mature sequence shown here represents the most commonly cloned form from large-scale cloning studies [3].

Genome context

• Coordinates (GRCm38): chr12: 109715318-109715402 [+]
• Overlapping transcripts:

  intergenic

Clustered miRNAs

< 10kb from mmu-mir-494

• mmu-mir-379: chr12: 109709060-109709125 [+]
• mmu-mir-411: chr12: 109710175-109710256 [+]
• mmu-mir-299a: chr12: 109710638-109710700 [+]
• mmu-mir-299b: chr12: 109710645-109710693 [-]
• mmu-mir-380: chr12: 109711803-109711863 [+]
• mmu-mir-1197: chr12: 109712317-109712436 [+]
• mmu-mir-323: chr12: 109712508-109712593 [+]
• mmu-mir-758: chr12: 109712810-109712890 [+]
• mmu-mir-329: chr12: 109713481-109713577 [+]
• mmu-mir-494: chr12: 109715318-109715402 [+]
• mmu-mir-679: chr12: 109715577-109715650 [+]
• mmu-mir-1193: chr12: 109715671-109715791 [+]
• mmu-mir-666: chr12: 109717085-109717183 [+]
• mmu-mir-543: chr12: 109717258-109717333 [+]
• mmu-mir-495: chr12: 109718754-109718816 [+]
• mmu-mir-667: chr12: 109720006-109720097 [+]
• mmu-mir-376c: chr12: 109722718-109722803 [+]
• mmu-mir-654: chr12: 109723218-109723301 [+]
• mmu-mir-376b: chr12: 109723458-109723539 [+]
• mmu-mir-376a: chr12: 109723781-109723848 [+]
• mmu-mir-300: chr12: 109724313-109724391 [+]

Database links

• ENTREZGENE: 723878;Mir494
• MGI: 3619426;Mir494

Mature sequence mmu-miR-494-5p

• Accession: MIMAT0017215
• Previous IDs: mmu-miR-494*
• Sequence: 17 - agguuguccguguugucuucuc - 38
• Deep sequencing: 70 reads, 19 experiments
• Evidence: experimental; Solexa [5]
• Predicted targets:
  • DIANA-MICROT:mmu-miR-494-5p
  • MIRDB:mmu-miR-494-5p
  • RNA22-MMU:mmu-miR-494-5p

Mature sequence mmu-miR-494-3p

• Accession: MIMAT0003182
• Previous IDs: mmu-miR-494
• Sequence: 50 - ugaaacauacacgggaaaccuc - 71
• Deep sequencing: 16474 reads, 64 experiments
• Evidence: experimental; cloned [1,3], MPSS [2], Solexa [4-5]
• Predicted targets:
  • DIANA-MICROT:mmu-miR-494-3p
  • MICRORNA.ORG:mmu-miR-494-3p
  • MIRDB:mmu-miR-494-3p
  • RNA22-MMU:mmu-miR-494-3p
  • TARGETSCAN-VERT:mmu-miR-494

References

1. PMID:16274478 "Identification of clustered microRNAs using an ab initio prediction method" Sewer A, Paul N, Landgraf P, Aravin A, Pfeffer S, Brownstein MJ, Tuschl T, van Nimwegen E, Zavolan M BMC Bioinformatics. 6:267(2005).
2. PMID:16582102 "The expression profile of microRNAs in mouse embryos" Mineno J, Okamoto S, Ando T, Sato M, Chono H, Izu H, Takayama M, Asada K, Mirochnitchenko O, Inouye M, Kato I Nucleic Acids Res. 34:1765-1771(2006).
3. PMID:17604727 "A mammalian microRNA expression atlas based on small RNA library sequencing" Landgraf P, Rusu M, Sheridan R, Sewer A, Iovino N, Aravin A, Pfeffer S, Rice A, Kamphorst AO, Landthaler M, Lin C, Socci ND, Hermida L, Fulci V, Chiaretti S, Foa R, Schliwka J, Fuchs U, Novosel A, Muller RU, Schermer B, Bissels U, Inman J, Phan Q, Chien M Cell. 129:1401-1414(2007).
4. PMID:20215419 "MicroRNA transcriptome in the newborn mouse ovaries determined by massive parallel sequencing" Ahn HW, Morin RD, Zhao H, Harris RA, Coarfa C, Chen ZJ, Milosavljevic A, Marra MA, Rajkovic A Mol Hum Reprod. 16:463-471(2010).
5. PMID:20413612 "Mammalian microRNAs: experimental evaluation of novel and previously annotated genes" Chiang HR, Schoenfeld LW, Ruby JG, Auyeung VC, Spies N, Baek D, Johnston WK, Russ C, Luo S, Babiarz JE, Blelloch R, Schroth GP, Nusbaum C, Bartel DP Genes Dev. 24:992-1009(2010).