Stem-loop sequence hsa-mir-376a-2

Accession

MI0003529

Symbol

HGNC:MIR376A2

Description

Homo sapiens miR-376a-2 stem-loop

Gene family

MIPF0000091; mir-368

Stem-loop

      u    g  a         u         --   g 
gguauu aaaa gu gauuuuccu cuaugguua  cgu u
|||||| |||| || ||||||||| |||||||||  ||| u
cuauga uuuu ca cuaaaagga gauacuaau  gua u
      c    g  c         -         ug   g 

Deep sequencing

269 reads, 21 experiments

Comments

The mature miR-376a products have been shown to be modified by A to I edits [2].

Genome context

• Coordinates (GRCh37.p5): chr14: 101506406-101506485 [+]
• Overlapping transcripts:

  intergenic

Clustered miRNAs

< 10kb from hsa-mir-376a-2

• hsa-mir-543: chr14: 101498324-101498401 [+]
• hsa-mir-495: chr14: 101500092-101500173 [+]
• hsa-mir-376c: chr14: 101506027-101506092 [+]
• hsa-mir-376a-2: chr14: 101506406-101506485 [+]
• hsa-mir-654: chr14: 101506556-101506636 [+]
• hsa-mir-376b: chr14: 101506773-101506872 [+]
• hsa-mir-376a-1: chr14: 101507119-101507186 [+]
• hsa-mir-300: chr14: 101507700-101507782 [+]
• hsa-mir-1185-1: chr14: 101509314-101509399 [+]
• hsa-mir-1185-2: chr14: 101510535-101510620 [+]
• hsa-mir-381: chr14: 101512257-101512331 [+]
• hsa-mir-487b: chr14: 101512792-101512875 [+]
• hsa-mir-539: chr14: 101513658-101513735 [+]
• hsa-mir-889: chr14: 101514238-101514316 [+]
• hsa-mir-544a: chr14: 101514995-101515085 [+]
• hsa-mir-655: chr14: 101515887-101515983 [+]

Database links

• EMBL: AY785912;AY785912
• ENTREZGENE: 664615;MIR376A2
• HGNC: 32532;MIR376A2

Mature sequence hsa-miR-376a-2-5p

• Accession: MIMAT0022928
• Sequence: 12 - gguagauuuuccuucuauggu - 32
• Deep sequencing: 5 reads, 3 experiments
• Evidence: experimental; SOLiD [4]

Mature sequence hsa-miR-376a-3p

• Accession: MIMAT0000729
• Previous IDs: hsa-miR-376a
• Sequence: 50 - aucauagaggaaaauccacgu - 70
• Deep sequencing: 527 reads, 20 experiments
• Evidence: experimental; cloned [1,3]
• Validated targets:
  • TARBASE:hsa-miR-376a-3p
• Predicted targets:
  • DIANA-MICROT:hsa-miR-376a-3p
  • MICRORNA.ORG:hsa-miR-376a-3p
  • MIRDB:hsa-miR-376a-3p
  • RNA22-HSA:hsa-miR-376a-3p
  • TARGETMINER:hsa-miR-376a-3p
  • TARGETSCAN-VERT:hsa-miR-376a

References

1. PMID:15891114 "Clustering and conservation patterns of human microRNAs" Altuvia Y, Landgraf P, Lithwick G, Elefant N, Pfeffer S, Aravin A, Brownstein MJ, Tuschl T, Margalit H Nucleic Acids Res. 33:2697-2706(2005).
2. PMID:17322061 "Redirection of silencing targets by adenosine-to-inosine editing of miRNAs" Kawahara Y, Zinshteyn B, Sethupathy P, Iizasa H, Hatzigeorgiou AG, Nishikura K Science. 315:1137-1140(2007).
3. PMID:17604727 "A mammalian microRNA expression atlas based on small RNA library sequencing" Landgraf P, Rusu M, Sheridan R, Sewer A, Iovino N, Aravin A, Pfeffer S, Rice A, Kamphorst AO, Landthaler M, Lin C, Socci ND, Hermida L, Fulci V, Chiaretti S, Foa R, Schliwka J, Fuchs U, Novosel A, Muller RU, Schermer B, Bissels U, Inman J, Phan Q, Chien M Cell. 129:1401-1414(2007).
4. PMID:22282338 "Deep-sequencing of endothelial cells exposed to hypoxia reveals the complexity of known and novel microRNAs" Voellenkle C, Rooij Jv, Guffanti A, Brini E, Fasanaro P, Isaia E, Croft L, David M, Capogrossi MC, Moles A, Felsani A, Martelli F RNA. 18:472-484(2012).