Stem-loop sequence mml-mir-127

Accession

MI0002582 (change log)

Description

Macaca mulatta miR-127 stem-loop

Gene family

MIPF0000080; mir-127

Stem-loop

   -----ugu   ca   ucu      ugcug         g  c      --  a 
5'         gau  cug   ccagcc     aagcucaga gg ucugau  uc g
           |||  |||   ||||||     ||||||||| || ||||||  || a
3'         cug  ggc   ggucgg     uucgagucu cc aggcua  ag a
   cuacuccu   aa   --u      -----         g  u      cu  a 

Deep sequencing

273107 reads, 1.33e+03 reads per million, 9 experiments

Confidence

Annotation confidence: high

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Comments

Berezikov et al. used primers designed from human miRNA gene flanking sequence to amplify miRNA precursor regions in primates [1]. The expression of the mature miRNA was not validated.

Genome context

• Coordinates (Mmul_8.0.1; GCA_000772875.3): chr7: 163217177-163217273 [+]
• Overlapping transcripts:

  intergenic

Clustered miRNAs

< 10kb from mml-mir-127

• mml-mir-337: chr7: 163207975-163208067 [+]
• mml-mir-665: chr7: 163208516-163208587 [+]
• mml-mir-431: chr7: 163215205-163215318 [+]
• mml-mir-433: chr7: 163216084-163216176 [+]
• mml-mir-127: chr7: 163217177-163217273 [+]
• mml-mir-432: chr7: 163218684-163218777 [+]
• mml-mir-136: chr7: 163218898-163218989 [+]

Database links

• EntrezGene:MIR127
• RNAcentral:URS000061020A_9544
• miRBase Tracker:MI0002582

Mature sequence mml-miR-127-5p

• Accession: MIMAT0026570
• Sequence: 24 - ugaagcucagagggcucugauu - 45
• Deep sequencing: 14371 reads, 9 experiments
• Evidence: experimental; Illumina [2]
• Database links:
  • RNAcentral:URS0000780B3D_9544
• Predicted targets:
  • TargetScanVert:mml-miR-127-5p

Mature sequence mml-miR-127-3p

• Accession: MIMAT0002281
• Sequence: 57 - ucggauccgucugagcuuggcu - 78
• Deep sequencing: 258735 reads, 9 experiments
• Evidence: experimental; Illumina [2]
• Database links:
  • RNAcentral:URS00001E3DAA_9544
• Predicted targets:
  • TargetScanVert:mml-miR-127-3p

References

1. PMID:15652478 "Phylogenetic shadowing and computational identification of human microRNA genes" Berezikov E, Guryev V, van de Belt J, Wienholds E, Plasterk RH, Cuppen E Cell. 120:21-24(2005).
2. PMID:23034410 "Birth and expression evolution of mammalian microRNA genes" Meunier J, Lemoine F, Soumillon M, Liechti A, Weier M, Guschanski K, Hu H, Khaitovich P, Kaessmann H Genome Res. 23:34-45(2013).