Stem-loop sequence hsa-mir-376b

Accession

MI0002466

Symbol

HGNC:MIR376B

Description

Homo sapiens miR-376b stem-loop

Gene family

MIPF0000091; mir-368

Stem-loop

    ccuuc         u           a     u     uuuacg    u 
cagu     uuugguauu aaaacguggau uuccu cuaug      ugau c
||||     ||||||||| ||||||||||| ||||| |||||      |||| c
gucg     aaacuauga uuuuguaccua aagga gauac      auug u
    ----u         c           a     -     ----ua    g 

Deep sequencing

279 reads, 21 experiments

Comments

The mature miR-376b products have been shown to be modified by A to I edits [2].

Genome context

• Coordinates (GRCh37.p5): chr14: 101506773-101506872 [+]
• Overlapping transcripts:

  intergenic

Clustered miRNAs

< 10kb from hsa-mir-376b

• hsa-mir-543: chr14: 101498324-101498401 [+]
• hsa-mir-495: chr14: 101500092-101500173 [+]
• hsa-mir-376c: chr14: 101506027-101506092 [+]
• hsa-mir-376a-2: chr14: 101506406-101506485 [+]
• hsa-mir-654: chr14: 101506556-101506636 [+]
• hsa-mir-376b: chr14: 101506773-101506872 [+]
• hsa-mir-376a-1: chr14: 101507119-101507186 [+]
• hsa-mir-300: chr14: 101507700-101507782 [+]
• hsa-mir-1185-1: chr14: 101509314-101509399 [+]
• hsa-mir-1185-2: chr14: 101510535-101510620 [+]
• hsa-mir-381: chr14: 101512257-101512331 [+]
• hsa-mir-487b: chr14: 101512792-101512875 [+]
• hsa-mir-539: chr14: 101513658-101513735 [+]
• hsa-mir-889: chr14: 101514238-101514316 [+]
• hsa-mir-544a: chr14: 101514995-101515085 [+]
• hsa-mir-655: chr14: 101515887-101515983 [+]

Database links

• ENTREZGENE: 574435;MIR376B
• HGNC: 32066;MIR376B

Mature sequence hsa-miR-376b-5p

• Accession: MIMAT0022923
• Sequence: 24 - cguggauauuccuucuauguuu - 45
• Deep sequencing: 14 reads, 8 experiments
• Evidence: experimental; SOLiD [4]

Mature sequence hsa-miR-376b-3p

• Accession: MIMAT0002172
• Previous IDs: hsa-miR-376b
• Sequence: 62 - aucauagaggaaaauccauguu - 83
• Deep sequencing: 265 reads, 20 experiments
• Evidence: experimental; cloned [3], SOLiD [4]
• Validated targets:
  • TARBASE:hsa-miR-376b-3p
• Predicted targets:
  • DIANA-MICROT:hsa-miR-376b-3p
  • MICRORNA.ORG:hsa-miR-376b-3p
  • MIRDB:hsa-miR-376b-3p
  • RNA22-HSA:hsa-miR-376b-3p
  • TARGETSCAN-VERT:hsa-miR-376b

References

1. PMID:15891114 "Clustering and conservation patterns of human microRNAs" Altuvia Y, Landgraf P, Lithwick G, Elefant N, Pfeffer S, Aravin A, Brownstein MJ, Tuschl T, Margalit H Nucleic Acids Res. 33:2697-2706(2005).
2. PMID:17322061 "Redirection of silencing targets by adenosine-to-inosine editing of miRNAs" Kawahara Y, Zinshteyn B, Sethupathy P, Iizasa H, Hatzigeorgiou AG, Nishikura K Science. 315:1137-1140(2007).
3. PMID:17604727 "A mammalian microRNA expression atlas based on small RNA library sequencing" Landgraf P, Rusu M, Sheridan R, Sewer A, Iovino N, Aravin A, Pfeffer S, Rice A, Kamphorst AO, Landthaler M, Lin C, Socci ND, Hermida L, Fulci V, Chiaretti S, Foa R, Schliwka J, Fuchs U, Novosel A, Muller RU, Schermer B, Bissels U, Inman J, Phan Q, Chien M Cell. 129:1401-1414(2007).
4. PMID:22282338 "Deep-sequencing of endothelial cells exposed to hypoxia reveals the complexity of known and novel microRNAs" Voellenkle C, Rooij Jv, Guffanti A, Brini E, Fasanaro P, Isaia E, Croft L, David M, Capogrossi MC, Moles A, Felsani A, Martelli F RNA. 18:472-484(2012).