Stem-loop sequence mmu-mir-412

Accession

MI0001164

Symbol

MGI:Mir412

Description

Mus musculus miR-412 stem-loop

Gene family

MIPF0000192; mir-412

Stem-loop

      g g    a    c      c   aa    -    u 
ggguau g acgg uggu gaccag ugg  agua auug u
|||||| | |||| |||| |||||| |||  |||| ||||  
cccgug c ugcc auca cugguc acu  ucau uaau u
      g -    g    c      c   --    g    c 

Deep sequencing

2198 reads, 35 experiments

Comments

Seitz et al. predicted a cluster of 40 miRNAs in the imprinted human 14q32 domain, and confirmed the expression of a subset by Northern blot or primer extension in mouse [1]. The mature sequence shown here represents the most commonly cloned form from large-scale cloning studies [3]. The 5' end of the miRNA may be offset with respect to previous annotations.

Genome context

• Coordinates (GRCm38): chr12: 109743289-109743368 [+]
• Overlapping transcripts:

  intergenic

Clustered miRNAs

< 10kb from mmu-mir-412

• mmu-mir-382: chr12: 109733771-109733846 [+]
• mmu-mir-134: chr12: 109734139-109734209 [+]
• mmu-mir-668: chr12: 109734732-109734797 [+]
• mmu-mir-485: chr12: 109734902-109734974 [+]
• mmu-mir-453: chr12: 109735619-109735700 [+]
• mmu-mir-154: chr12: 109738433-109738498 [+]
• mmu-mir-496a: chr12: 109739119-109739197 [+]
• mmu-mir-377: chr12: 109740510-109740577 [+]
• mmu-mir-541: chr12: 109742409-109742498 [+]
• mmu-mir-409: chr12: 109743158-109743236 [+]
• mmu-mir-412: chr12: 109743289-109743368 [+]
• mmu-mir-369: chr12: 109743418-109743496 [+]
• mmu-mir-410: chr12: 109743715-109743795 [+]
• mmu-mir-3072: chr12: 109747878-109747960 [+]

Database links

• ENTREZGENE: 723913;Mir412
• MGI: 3619400;Mir412

Mature sequence mmu-miR-412-5p

• Accession: MIMAT0017173
• Sequence: 15 - uggucgaccagcuggaaaguaau - 37
• Deep sequencing: 1557 reads, 33 experiments
• Evidence: experimental; Solexa [5]
• Predicted targets:
  • DIANA-MICROT:mmu-miR-412-5p
  • MIRDB:mmu-miR-412-5p
  • RNA22-MMU:mmu-miR-412-5p

Mature sequence mmu-miR-412-3p

• Accession: MIMAT0001094
• Previous IDs: mmu-miR-412
• Sequence: 52 - uucaccugguccacuagccg - 71
• Deep sequencing: 641 reads, 30 experiments
• Evidence: experimental; PCR [1], cloned [2-3], Solexa [4-5]
• Predicted targets:
  • DIANA-MICROT:mmu-miR-412-3p
  • MICRORNA.ORG:mmu-miR-412-3p
  • MIRDB:mmu-miR-412-3p
  • RNA22-MMU:mmu-miR-412-3p
  • TARGETSCAN-VERT:mmu-miR-412

References

1. PMID:15310658 "A large imprinted microRNA gene cluster at the mouse Dlk1-Gtl2 domain" Seitz H, Royo H, Bortolin ML, Lin SP, Ferguson-Smith AC, Cavaille J Genome Res. 14:1741-1748(2004).
2. PMID:16274478 "Identification of clustered microRNAs using an ab initio prediction method" Sewer A, Paul N, Landgraf P, Aravin A, Pfeffer S, Brownstein MJ, Tuschl T, van Nimwegen E, Zavolan M BMC Bioinformatics. 6:267(2005).
3. PMID:17604727 "A mammalian microRNA expression atlas based on small RNA library sequencing" Landgraf P, Rusu M, Sheridan R, Sewer A, Iovino N, Aravin A, Pfeffer S, Rice A, Kamphorst AO, Landthaler M, Lin C, Socci ND, Hermida L, Fulci V, Chiaretti S, Foa R, Schliwka J, Fuchs U, Novosel A, Muller RU, Schermer B, Bissels U, Inman J, Phan Q, Chien M Cell. 129:1401-1414(2007).
4. PMID:20215419 "MicroRNA transcriptome in the newborn mouse ovaries determined by massive parallel sequencing" Ahn HW, Morin RD, Zhao H, Harris RA, Coarfa C, Chen ZJ, Milosavljevic A, Marra MA, Rajkovic A Mol Hum Reprod. 16:463-471(2010).
5. PMID:20413612 "Mammalian microRNAs: experimental evaluation of novel and previously annotated genes" Chiang HR, Schoenfeld LW, Ruby JG, Auyeung VC, Spies N, Baek D, Johnston WK, Russ C, Luo S, Babiarz JE, Blelloch R, Schroth GP, Nusbaum C, Bartel DP Genes Dev. 24:992-1009(2010).