Stem-loop sequence mmu-mir-376b

Accession

MI0001162

Symbol

MGI:Mir376b

Description

Mus musculus miR-376b stem-loop

Gene family

MIPF0000091; mir-368

Stem-loop

u      u           a     u         cgug  u 
 gguauu aaaagguggau uuccu cuaugguua    cu c
 |||||| ||||||||||| ||||| |||||||||    ||  
 cuauga uuuuucaccua aagga gauacuaau    gg c
a      c           c     -         ---a  u 

Deep sequencing

52185 reads, 63 experiments

Comments

Seitz et al. predicted a cluster of 40 miRNAs in the imprinted human 14q32 domain, and confirmed the expression of a subset by Northern blot or primer extension in mouse [1]. The mature miR-376b products have been shown to be modified by A to I edits [3]. The mature sequence shown here represents the most commonly cloned form from large-scale cloning studies [4].

Genome context

• Coordinates (GRCm38): chr12: 109723458-109723539 [+]
• Overlapping transcripts:

  sense

  ENSMUST00000102052 ; Mir376b-201; exon 1

Clustered miRNAs

< 10kb from mmu-mir-376b

• mmu-mir-329: chr12: 109713481-109713577 [+]
• mmu-mir-494: chr12: 109715318-109715402 [+]
• mmu-mir-679: chr12: 109715577-109715650 [+]
• mmu-mir-1193: chr12: 109715671-109715791 [+]
• mmu-mir-666: chr12: 109717085-109717183 [+]
• mmu-mir-543: chr12: 109717258-109717333 [+]
• mmu-mir-495: chr12: 109718754-109718816 [+]
• mmu-mir-667: chr12: 109720006-109720097 [+]
• mmu-mir-376c: chr12: 109722718-109722803 [+]
• mmu-mir-654: chr12: 109723218-109723301 [+]
• mmu-mir-376b: chr12: 109723458-109723539 [+]
• mmu-mir-376a: chr12: 109723781-109723848 [+]
• mmu-mir-300: chr12: 109724313-109724391 [+]
• mmu-mir-381: chr12: 109726822-109726896 [+]
• mmu-mir-487b: chr12: 109727333-109727414 [+]
• mmu-mir-539: chr12: 109728129-109728202 [+]
• mmu-mir-544: chr12: 109729325-109729402 [+]

Database links

• ENTREZGENE: 723934;Mir376b
• MGI: 3619378;Mir376b

Mature sequence mmu-miR-376b-5p

• Accession: MIMAT0003388
• Previous IDs: mmu-miR-376b*
• Sequence: 14 - guggauauuccuucuaugguua - 35
• Deep sequencing: 18720 reads, 45 experiments
• Evidence: experimental; cloned [2,4], Solexa [5-6]
• Predicted targets:
  • DIANA-MICROT:mmu-miR-376b-5p
  • MICRORNA.ORG:mmu-miR-376b-5p
  • MIRDB:mmu-miR-376b-5p
  • RNA22-MMU:mmu-miR-376b-5p

Mature sequence mmu-miR-376b-3p

• Accession: MIMAT0001092
• Previous IDs: mmu-miR-376b
• Sequence: 51 - aucauagaggaacauccacuu - 71
• Deep sequencing: 33446 reads, 55 experiments
• Evidence: experimental; Northern [1], PCR [1], cloned [2,4], Solexa [5-6]
• Predicted targets:
  • DIANA-MICROT:mmu-miR-376b-3p
  • MICRORNA.ORG:mmu-miR-376b-3p
  • MIRDB:mmu-miR-376b-3p
  • RNA22-MMU:mmu-miR-376b-3p
  • TARGETSCAN-VERT:mmu-miR-376b

References

1. PMID:15310658 "A large imprinted microRNA gene cluster at the mouse Dlk1-Gtl2 domain" Seitz H, Royo H, Bortolin ML, Lin SP, Ferguson-Smith AC, Cavaille J Genome Res. 14:1741-1748(2004).
2. PMID:16274478 "Identification of clustered microRNAs using an ab initio prediction method" Sewer A, Paul N, Landgraf P, Aravin A, Pfeffer S, Brownstein MJ, Tuschl T, van Nimwegen E, Zavolan M BMC Bioinformatics. 6:267(2005).
3. PMID:17322061 "Redirection of silencing targets by adenosine-to-inosine editing of miRNAs" Kawahara Y, Zinshteyn B, Sethupathy P, Iizasa H, Hatzigeorgiou AG, Nishikura K Science. 315:1137-1140(2007).
4. PMID:17604727 "A mammalian microRNA expression atlas based on small RNA library sequencing" Landgraf P, Rusu M, Sheridan R, Sewer A, Iovino N, Aravin A, Pfeffer S, Rice A, Kamphorst AO, Landthaler M, Lin C, Socci ND, Hermida L, Fulci V, Chiaretti S, Foa R, Schliwka J, Fuchs U, Novosel A, Muller RU, Schermer B, Bissels U, Inman J, Phan Q, Chien M Cell. 129:1401-1414(2007).
5. PMID:20215419 "MicroRNA transcriptome in the newborn mouse ovaries determined by massive parallel sequencing" Ahn HW, Morin RD, Zhao H, Harris RA, Coarfa C, Chen ZJ, Milosavljevic A, Marra MA, Rajkovic A Mol Hum Reprod. 16:463-471(2010).
6. PMID:20413612 "Mammalian microRNAs: experimental evaluation of novel and previously annotated genes" Chiang HR, Schoenfeld LW, Ruby JG, Auyeung VC, Spies N, Baek D, Johnston WK, Russ C, Luo S, Babiarz JE, Blelloch R, Schroth GP, Nusbaum C, Bartel DP Genes Dev. 24:992-1009(2010).