Stem-loop sequence rno-mir-181a-2

AccessionMI0000925 (change log)
Previous IDsrno-mir-181a
DescriptionRattus norvegicus miR-181a-2 stem-loop
Gene family MIPF0000007; mir-181
Community annotation

This text is a summary paragraph taken from the Wikipedia entry entitled mir-181_microRNA_precursor. miRBase and Rfam are facilitating community annotation of microRNA families and entries in Wikipedia. Read more ...

In molecular biology miR-181 microRNA precursor is a small non-coding RNA molecule. MicroRNAs (miRNAs) are transcribed as ~70 nucleotide precursors and subsequently processed by the RNase-III type enzyme Dicer to give a ~22 nucleotide mature product. In this case the mature sequence comes from the 5' arm of the precursor. They target and modulate protein expression by inhibiting translation and / or inducing degradation of target messenger RNAs. This new class of genes has recently been shown to play a central role in malignant transformation. miRNA are downregulated in many tumors and thus appear to function as tumor suppressor genes. The mature products miR-181a, miR-181b, miR-181c or miR-181d are thought to have regulatory roles at posttranscriptional level, through complementarity to target mRNAs. miR-181 which has been predicted or experimentally confirmed in a wide number of vertebrate species as rat, zebrafish, and in the pufferfish (see below) (MIPF0000007).

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Stem-loop
   agaugggcaaccaaggcagccuuaa     cu   u  a   u      cu  c    a    gggauuca 
5'                          gagga  cca gg aca ucaacg  gu ggug guuu        a
                            |||||  ||| || ||| ||||||  || |||| ||||         
3'                          uucuu  ggu cc ugu aguugc  ca ccac caaa        a
   ------------------------a     ag   u  a   c      --  a    -    aaaaacaa 
Get sequence
Deep sequencing
2679932 reads, 2.05e+04 reads per million, 39 experiments
Confidence Annotation confidence: high
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Genome context
Coordinates (Rnor_6.0; GCA_000001895.4) Overlapping transcripts
chr3: 23150352-23150468 [+]
antisense
ENSRNOT00000072113 ; Nr6a1-203; intron 1
ENSRNOT00000074794 ; Nr6a1-202; intron 2
Clustered miRNAs
< 10kb from rno-mir-181a-2
rno-mir-181a-2chr3: 23150352-23150468 [+]
rno-mir-181b-2chr3: 23151455-23151542 [+]
Database links

Mature sequence rno-miR-181a-5p

Accession MIMAT0000858
Previous IDsrno-miR-181a
Sequence

39 - 

aacauucaacgcugucggugagu

 - 61

Get sequence
Deep sequencing5354449 reads, 39 experiments
Evidence experimental; cloned [3-4], SOLiD [4]
Predicted targets

Mature sequence rno-miR-181a-2-3p

Accession MIMAT0017138
Previous IDsrno-miR-181a-2*
Sequence

84 - 

accaccaaccguugacugu

 - 102

Get sequence
Deep sequencing2787 reads, 37 experiments
Evidence experimental; SOLiD [4]
Predicted targets

References

1
PMID:14691248 "Identification of many microRNAs that copurify with polyribosomes in mammalian neurons" Kim J, Krichevsky A, Grad Y, Hayes GD, Kosik KS, Church GM, Ruvkun G Proc Natl Acad Sci U S A. 101:360-365(2004).
2
PMID:17604727 "A mammalian microRNA expression atlas based on small RNA library sequencing" Landgraf P, Rusu M, Sheridan R, Sewer A, Iovino N, Aravin A, Pfeffer S, Rice A, Kamphorst AO, Landthaler M, Lin C, Socci ND, Hermida L, Fulci V, Chiaretti S, Foa R, Schliwka J, Fuchs U, Novosel A, Muller RU, Schermer B, Bissels U, Inman J, Phan Q, Chien M Cell. 129:1401-1414(2007).
3
PMID:17805466 "Cloning and identification of novel microRNAs from rat hippocampus" He X, Zhang Q, Liu Y, Pan X Acta Biochim Biophys Sin (Shanghai). 39:708-714(2007).
4
PMID:20403161 "Small RNA expression and strain specificity in the rat" Linsen SE, de Wit E, de Bruijn E, Cuppen E BMC Genomics. 11:249(2010).