Stem-loop sequence mmu-mir-380

Accession

MI0000797

Symbol

MGI:Mir380

Description

Mus musculus miR-380 stem-loop

Gene family

MIPF0000126; mir-379

Stem-loop

      guu       ga       c   u 
aagaug   gaccaua  acaugcg uac u
||||||   |||||||  ||||||| |||  
uucuac   cugguau  uguaugc gug c
      -ac       ga       u   u 

Deep sequencing

29646 reads, 55 experiments

Comments

Seitz et al. predicted a cluster of 40 miRNAs in the imprinted human 14q32 domain, and confirmed the expression of a subset by Northern blot or primer extension in mouse, including a sequence from the 3' arm of this precursor [1]. Poy et al. cloned a sequence from the 5' arm from mouse pancreatic beta cell line MIN6 [2]. The mature sequence shown here represents the most commonly cloned form from large-scale cloning studies [3]. The 5' end of the miRNA may be offset with respect to previous annotations.

Genome context

• Coordinates (GRCm38): chr12: 109711803-109711863 [+]
• Overlapping transcripts:

  antisense

  ENSMUST00000175483 ; AC121784.3-201; exon 1

Clustered miRNAs

< 10kb from mmu-mir-380

• mmu-mir-379: chr12: 109709060-109709125 [+]
• mmu-mir-411: chr12: 109710175-109710256 [+]
• mmu-mir-299a: chr12: 109710638-109710700 [+]
• mmu-mir-299b: chr12: 109710645-109710693 [-]
• mmu-mir-380: chr12: 109711803-109711863 [+]
• mmu-mir-1197: chr12: 109712317-109712436 [+]
• mmu-mir-323: chr12: 109712508-109712593 [+]
• mmu-mir-758: chr12: 109712810-109712890 [+]
• mmu-mir-329: chr12: 109713481-109713577 [+]
• mmu-mir-494: chr12: 109715318-109715402 [+]
• mmu-mir-679: chr12: 109715577-109715650 [+]
• mmu-mir-1193: chr12: 109715671-109715791 [+]
• mmu-mir-666: chr12: 109717085-109717183 [+]
• mmu-mir-543: chr12: 109717258-109717333 [+]
• mmu-mir-495: chr12: 109718754-109718816 [+]
• mmu-mir-667: chr12: 109720006-109720097 [+]

Database links

• ENTREZGENE: 723859;Mir380
• MGI: 3619389;Mir380

Mature sequence mmu-miR-380-5p

• Accession: MIMAT0000744
• Sequence: 4 - augguugaccauagaacaugcg - 25
• Deep sequencing: 4480 reads, 43 experiments
• Evidence: experimental; cloned [2-3], Solexa [4-5]
• Predicted targets:
  • DIANA-MICROT:mmu-miR-380-5p
  • MICRORNA.ORG:mmu-miR-380-5p
  • MIRDB:mmu-miR-380-5p
  • RNA22-MMU:mmu-miR-380-5p
  • TARGETSCAN-VERT:mmu-miR-380-5p

Mature sequence mmu-miR-380-3p

• Accession: MIMAT0000745
• Sequence: 40 - uauguaguaugguccacaucuu - 61
• Deep sequencing: 25158 reads, 49 experiments
• Evidence: experimental; Northern [1], PCR [1], cloned [3], Solexa [4]
• Predicted targets:
  • DIANA-MICROT:mmu-miR-380-3p
  • MICRORNA.ORG:mmu-miR-380-3p
  • MIRDB:mmu-miR-380-3p
  • RNA22-MMU:mmu-miR-380-3p
  • TARGETSCAN-VERT:mmu-miR-380-3p

References

1. PMID:15310658 "A large imprinted microRNA gene cluster at the mouse Dlk1-Gtl2 domain" Seitz H, Royo H, Bortolin ML, Lin SP, Ferguson-Smith AC, Cavaille J Genome Res. 14:1741-1748(2004).
2. PMID:15538371 "A pancreatic islet-specific microRNA regulates insulin secretion" Poy MN, Eliasson L, Krutzfeldt J, Kuwajima S, Ma X, Macdonald PE, Pfeffer S, Tuschl T, Rajewsky N, Rorsman P, Stoffel M Nature. 432:226-230(2004).
3. PMID:17604727 "A mammalian microRNA expression atlas based on small RNA library sequencing" Landgraf P, Rusu M, Sheridan R, Sewer A, Iovino N, Aravin A, Pfeffer S, Rice A, Kamphorst AO, Landthaler M, Lin C, Socci ND, Hermida L, Fulci V, Chiaretti S, Foa R, Schliwka J, Fuchs U, Novosel A, Muller RU, Schermer B, Bissels U, Inman J, Phan Q, Chien M Cell. 129:1401-1414(2007).
4. PMID:20215419 "MicroRNA transcriptome in the newborn mouse ovaries determined by massive parallel sequencing" Ahn HW, Morin RD, Zhao H, Harris RA, Coarfa C, Chen ZJ, Milosavljevic A, Marra MA, Rajkovic A Mol Hum Reprod. 16:463-471(2010).
5. PMID:20413612 "Mammalian microRNAs: experimental evaluation of novel and previously annotated genes" Chiang HR, Schoenfeld LW, Ruby JG, Auyeung VC, Spies N, Baek D, Johnston WK, Russ C, Luo S, Babiarz JE, Blelloch R, Schroth GP, Nusbaum C, Bartel DP Genes Dev. 24:992-1009(2010).