Stem-loop sequence mmu-mir-200c

AccessionMI0000694
Symbol MGI:Mir200c
DescriptionMus musculus miR-200c stem-loop
Gene family MIPF0000019; mir-8
Community annotation

This text is a summary paragraph taken from the Wikipedia entry entitled mir-8/mir-141/mir-200_microRNA_precursor_family. miRBase and Rfam are facilitating community annotation of microRNA families and entries in Wikipedia. Read more ...

The miR-8 microRNA precursor (homologous to miR-141, miR-200, miR-236), is a short non-coding RNA gene involved in gene regulation. miR-8 in Drosophila melanogaster is expressed from the 3' arm of related precursor hairpins (represented here), along with miR-200, miR-236, miR-429 and human and mouse homolog miR-141. Members of this precursor family have now been predicted or experimentally confirmed in a wide range of species. The bounds of the precursors are predicted based on conservation and base pairing and are not generally known. The miR-200 family is highly conserved in bilaterian animals, with miR-8 as the sole homolog in Drosophila. This species has accordingly been used heavily in work looking to uncover the biological function of the miR-200 family. miR-8 has been observed at all developmental stages of the embryo, with a complete absence from cultured S2 cells of D. melanogaster. Its expression has been seen to be strongly upregulated in larvae and this expression then sustained through to adulthood.

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Stem-loop
     cu    -      a        u   u   gg 
5' cc  cguc uuaccc gcaguguu ggg gcu  u
   ||  |||| |||||| |||||||| ||| |||  u
3' gg  guag aauggg cgucauaa cuc uga  g
     ag    u      c        u   -   gg 
Get sequence
Deep sequencing
116605 reads, 1.05e+03 reads per million, 107 experiments
Confidence Annotation confidence: high
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Comments

Mouse mir-200c is predicted [3] based on homology to a cloned miR from human (MI0000650) [1]. Its expression in mouse was later verified independently [2,4]. The mature sequence shown here represents the most commonly cloned form from large-scale cloning studies [4].

Genome context
Coordinates (GRCm38) Overlapping transcripts
chr6: 124718322-124718390 [-]
sense
OTTMUST00000067268 ; RP24-297C1.9-001; intron 1
ENSMUST00000143765 ; Gm15884-001; intron 1
ENSMUST00000083528 ; Mir200c-201; exon 1
Clustered miRNAs
< 10kb from mmu-mir-200c
mmu-mir-200cchr6: 124718322-124718390 [-]
mmu-mir-141chr6: 124717914-124717985 [-]
Database links

Mature sequence mmu-miR-200c-5p

Accession MIMAT0004663
Previous IDsmmu-miR-200c*
Sequence

5 - 

cgucuuacccagcaguguuugg

 - 26

Get sequence
Deep sequencing95 reads, 19 experiments
Evidence experimental; cloned [4], Illumina [5-6]
Predicted targets

Mature sequence mmu-miR-200c-3p

Accession MIMAT0000657
Previous IDsmmu-miR-200c
Sequence

45 - 

uaauacugccggguaaugaugga

 - 67

Get sequence
Deep sequencing50754 reads, 86 experiments
Evidence experimental; cloned [2,4], Illumina [5-6]
Validated targets
Predicted targets

References

1
PMID:14573789 "Reduced accumulation of specific microRNAs in colorectal neoplasia" Michael MZ, O' Connor SM, van Holst Pellekaan NG, Young GP, James RJ Mol Cancer Res. 1:882-891(2003).
2
PMID:15538371 "A pancreatic islet-specific microRNA regulates insulin secretion" Poy MN, Eliasson L, Krutzfeldt J, Kuwajima S, Ma X, Macdonald PE, Pfeffer S, Tuschl T, Rajewsky N, Rorsman P, Stoffel M Nature. 432:226-230(2004).
3
4
PMID:17604727 "A mammalian microRNA expression atlas based on small RNA library sequencing" Landgraf P, Rusu M, Sheridan R, Sewer A, Iovino N, Aravin A, Pfeffer S, Rice A, Kamphorst AO, Landthaler M, Lin C, Socci ND, Hermida L, Fulci V, Chiaretti S, Foa R, Schliwka J, Fuchs U, Novosel A, Muller RU, Schermer B, Bissels U, Inman J, Phan Q, Chien M Cell. 129:1401-1414(2007).
5
PMID:20215419 "MicroRNA transcriptome in the newborn mouse ovaries determined by massive parallel sequencing" Ahn HW, Morin RD, Zhao H, Harris RA, Coarfa C, Chen ZJ, Milosavljevic A, Marra MA, Rajkovic A Mol Hum Reprod. 16:463-471(2010).
6
PMID:20413612 "Mammalian microRNAs: experimental evaluation of novel and previously annotated genes" Chiang HR, Schoenfeld LW, Ruby JG, Auyeung VC, Spies N, Baek D, Johnston WK, Russ C, Luo S, Babiarz JE, Blelloch R, Schroth GP, Nusbaum C, Bartel DP Genes Dev. 24:992-1009(2010).