Stem-loop sequence mmu-mir-28a

Accession

MI0000690

Previous IDs

mmu-mir-28

Symbol

MGI:Mir28

Description

Mus musculus miR-28 stem-loop

Gene family

MIPF0000057; mir-28

Stem-loop

   c   a     a  g             ----uu     gcc 
ggu ccu ccuuc ag agcucacagucua      gaguu   u
||| ||| ||||| || |||||||||||||      |||||    
uca gga gggag uc ucgaguguuagau      cuuag   u
   c   c     g  g             cacccu     ucu 

Deep sequencing

121778 reads, 97 experiments

Comments

Mouse mir-28 is predicted [2] based on homology to a cloned miR from human (MI0000086 ) [1]. Its expression was later verified by cloning [3].

Genome context

• Coordinates (GRCm38): chr16: 24827855-24827940 [+]
• Overlapping transcripts:

  sense

  ENSMUST00000115314 ; Lpp-203; intron 3

  ENSMUST00000078988 ; Lpp-202; intron 7

  ENSMUST00000038053 ; Lpp-201; intron 8

Database links

• ENTREZGENE: 723830;Mir28
• MGI: 3619267;Mir28

Mature sequence mmu-miR-28a-5p

• Accession: MIMAT0000653
• Previous IDs: mmu-miR-28;mmu-miR-28-5p
• Sequence: 14 - aaggagcucacagucuauugag - 35
• Deep sequencing: 45808 reads, 82 experiments
• Evidence: experimental; cloned [3], Solexa [4-5]
• Validated targets:
  • TARBASE:mmu-miR-28a-5p
• Predicted targets:
  • DIANA-MICROT:mmu-miR-28a-5p
  • MICRORNA.ORG:mmu-miR-28a-5p
  • MIRDB:mmu-miR-28a-5p
  • RNA22-MMU:mmu-miR-28a-5p
  • TARGETSCAN-VERT:mmu-miR-28

Mature sequence mmu-miR-28a-3p

• Accession: MIMAT0004661
• Previous IDs: mmu-miR-28*;mmu-miR-28-3p
• Sequence: 54 - cacuagauugugagcugcugga - 75
• Deep sequencing: 21363 reads, 82 experiments
• Evidence: experimental; cloned [3], Solexa [4-5]
• Predicted targets:
  • DIANA-MICROT:mmu-miR-28a-3p
  • MICRORNA.ORG:mmu-miR-28a-3p
  • MIRDB:mmu-miR-28a-3p
  • RNA22-MMU:mmu-miR-28a-3p

References

1. PMID:11679670 "Identification of novel genes coding for small expressed RNAs" Lagos-Quintana M, Rauhut R, Lendeckel W, Tuschl T Science. 294:853-858(2001).
2. PMID:15634332 "New human and mouse microRNA genes found by homology search" Weber MJ FEBS J. 272:59-73(2005).
3. PMID:17604727 "A mammalian microRNA expression atlas based on small RNA library sequencing" Landgraf P, Rusu M, Sheridan R, Sewer A, Iovino N, Aravin A, Pfeffer S, Rice A, Kamphorst AO, Landthaler M, Lin C, Socci ND, Hermida L, Fulci V, Chiaretti S, Foa R, Schliwka J, Fuchs U, Novosel A, Muller RU, Schermer B, Bissels U, Inman J, Phan Q, Chien M Cell. 129:1401-1414(2007).
4. PMID:20215419 "MicroRNA transcriptome in the newborn mouse ovaries determined by massive parallel sequencing" Ahn HW, Morin RD, Zhao H, Harris RA, Coarfa C, Chen ZJ, Milosavljevic A, Marra MA, Rajkovic A Mol Hum Reprod. 16:463-471(2010).
5. PMID:20413612 "Mammalian microRNAs: experimental evaluation of novel and previously annotated genes" Chiang HR, Schoenfeld LW, Ruby JG, Auyeung VC, Spies N, Baek D, Johnston WK, Russ C, Luo S, Babiarz JE, Blelloch R, Schroth GP, Nusbaum C, Bartel DP Genes Dev. 24:992-1009(2010).