Stem-loop sequence osa-MIR156a

AccessionMI0000653
DescriptionOryza sativa miR156a stem-loop
Gene family MIPF0000008; MIR156
Community annotation

This text is a summary paragraph taken from the Wikipedia entry entitled mir-156_microRNA_precursor. miRBase and Rfam are facilitating community annotation of microRNA families and entries in Wikipedia. Read more ...

MicroRNA (miRNA) precursor mir-156 is a family of plant non-coding RNA. This microRNA has now been predicted or experimentally confirmed in a range of plant species (MIPF0000008). Animal miRNAs are transcribed as ~70 nucleotide precursors and subsequently processed by the Dicer enzyme to give a ~22 nucleotide product. In plants the precursor sequences may be longer, and the carpel factory (caf) enzyme appears to be involved in processing. In this case the mature sequence comes from the 5' arm of the precursor, and both Arabidopsis thaliana and rice genomes contain a number of related miRNA precursors which give rise to almost identical mature sequences. The extents of the hairpin precursors are not generally known and are estimated based on hairpin prediction. The products are thought to have regulatory roles through complementarity to mRNA.

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Stem-loop
        g       -     -        a      uguu   uu     aau 
5' ggagg ugacaga agaga gugagcac cguggu    ucc  gcaua   g
   ||||| ||||||| ||||| |||||||| ||||||    |||  |||||   a
3' ccucc acugucu ucucu cacucgug gcaucg    agg  cguau   u
        -       c     u        c      ----   uu     ccg 
Get sequence
Deep sequencing
21161280 reads, 2.72e+05 reads per million, 68 experiments
Confidence Annotation confidence: high
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Comments

The stem-loop sequence represented here is predicted based on homology to miRNAs cloned from Arabidopsis [1]. Its expression has not been verified in rice.

Genome context
Coordinates (MSU7) Overlapping transcripts
Chr1: 22524147-22524246 [-]
intergenic

Mature sequence osa-miR156a

Accession MIMAT0000618
Sequence

7 - 

ugacagaagagagugagcac

 - 26

Get sequence
Deep sequencing13863815 reads, 68 experiments
Evidence by similarity; MI0000183

References

1
PMID:12101121 "MicroRNAs in plants" Reinhart BJ, Weinstein EG, Rhoades MW, Bartel B, Bartel DP Genes Dev. 16:1616-1626(2002).