Stem-loop sequence mmu-let-7a-1

AccessionMI0000556
Symbol MGI:Mirlet7a-1
DescriptionMus musculus let-7a-1 stem-loop
Gene family MIPF0000002; let-7
Community annotation

This text is a summary paragraph taken from the Wikipedia entry entitled let-7_microRNA_precursor. miRBase and Rfam are facilitating community annotation of microRNA families and entries in Wikipedia. Read more ...

The Let-7 microRNA precursor was identified from a study of developmental timing in C. elegans, and was later shown to be part of a much larger class of non-coding RNAs termed microRNAs. miR-98 microRNA precursor from human is a let-7 family member. Let-7 miRNAs have now been predicted or experimentally confirmed in a wide range of species (MIPF0000002). miRNAs are initially transcribed in long transcripts (up to several hundred nucleotides) called primary miRNAs (pri-miRNAs), which are processed in the nucleus by Drosha and Pasha to hairpin structures of about ~70 nucleotide. These precursors (pre-miRNAs) are exported to the cytoplasm by exportin5, where they are subsequently processed by the enzyme Dicer to a ~22 nucleotide mature miRNA. The involvement of Dicer in miRNA processing demonstrates a relationship with the phenomenon of RNA interference.

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Stem-loop
   u     g     u   gu                uuagggucacac 
5'  ucacu uggga gag  aguagguuguauaguu            c
    ||||| ||||| |||  ||||||||||||||||            c
3'  agugg auccu uuc  ucaucuaacauaucaa            a
   u     a     -   ug                uagagggucacc 
Get sequence
Deep sequencing
94280304 reads, 2.8e+05 reads per million, 108 experiments
Confidence Annotation confidence: high
Feedback: Do you believe this miRNA is real?
Comments

The mature sequence shown here represents the most commonly cloned form from large-scale cloning studies [4].

Genome context
Coordinates (GRCm38) Overlapping transcripts
chr13: 48538179-48538272 [-]
sense
ENSMUST00000083487 ; Mirlet7a-1-201; exon 1
Clustered miRNAs
< 10kb from mmu-let-7a-1
mmu-let-7a-1chr13: 48538179-48538272 [-]
mmu-let-7f-1chr13: 48537829-48537917 [-]
mmu-let-7dchr13: 48536012-48536114 [-]
Database links

Mature sequence mmu-let-7a-5p

Accession MIMAT0000521
Previous IDsmmu-let-7a
Sequence

13 - 

ugagguaguagguuguauaguu

 - 34

Get sequence
Deep sequencing40462638 reads, 93 experiments
Evidence experimental; cloned [1-4], Illumina [5,7]
Validated targets
Predicted targets

Mature sequence mmu-let-7a-1-3p

Accession MIMAT0004620
Previous IDsmmu-let-7a*;mmu-let-7a-1*
Sequence

64 - 

cuauacaaucuacugucuuucc

 - 85

Get sequence
Deep sequencing2012 reads, 85 experiments
Evidence experimental; cloned [4], Illumina [5-6]
Predicted targets

References

1
PMID:12007417 "Identification of tissue-specific microRNAs from mouse" Lagos-Quintana M, Rauhut R, Yalcin A, Meyer J, Lendeckel W, Tuschl T Curr Biol. 12:735-739(2002).
2
PMID:15538371 "A pancreatic islet-specific microRNA regulates insulin secretion" Poy MN, Eliasson L, Krutzfeldt J, Kuwajima S, Ma X, Macdonald PE, Pfeffer S, Tuschl T, Rajewsky N, Rorsman P, Stoffel M Nature. 432:226-230(2004).
3
4
PMID:17604727 "A mammalian microRNA expression atlas based on small RNA library sequencing" Landgraf P, Rusu M, Sheridan R, Sewer A, Iovino N, Aravin A, Pfeffer S, Rice A, Kamphorst AO, Landthaler M, Lin C, Socci ND, Hermida L, Fulci V, Chiaretti S, Foa R, Schliwka J, Fuchs U, Novosel A, Muller RU, Schermer B, Bissels U, Inman J, Phan Q, Chien M Cell. 129:1401-1414(2007).
5
PMID:20215419 "MicroRNA transcriptome in the newborn mouse ovaries determined by massive parallel sequencing" Ahn HW, Morin RD, Zhao H, Harris RA, Coarfa C, Chen ZJ, Milosavljevic A, Marra MA, Rajkovic A Mol Hum Reprod. 16:463-471(2010).
6
PMID:20413612 "Mammalian microRNAs: experimental evaluation of novel and previously annotated genes" Chiang HR, Schoenfeld LW, Ruby JG, Auyeung VC, Spies N, Baek D, Johnston WK, Russ C, Luo S, Babiarz JE, Blelloch R, Schroth GP, Nusbaum C, Bartel DP Genes Dev. 24:992-1009(2010).