Stem-loop sequence mmu-mir-30c-2

AccessionMI0000548
Symbol MGI:Mir30c-2
DescriptionMus musculus miR-30c-2 stem-loop
Gene family MIPF0000005; mir-30
Community annotation

This text is a summary paragraph taken from the Wikipedia entry entitled mir-30_microRNA_precursor. miRBase and Rfam are facilitating community annotation of microRNA families and entries in Wikipedia. Read more ...

miR-30 microRNA precursor is a small non-coding RNA that regulates gene expression. Animal microRNAs are transcribed as pri-miRNA (primary miRNA) of varying length which in turns are processed in the nucleus by Drosha into ~70 nucleotide stem-loop precursor called pre-miRNA (preliminary miRNA) and subsequently processed by the Dicer enzyme to give a mature ~22 nucleotide product. In this case the mature sequence comes from both the 3' (miR-30) and 5' (mir-97-6) arms of the precursor. The products are thought to have regulatory roles through complementarity to mRNA. A screen of 17 miRNAs that have been predicted to regulate a number of breast cancer associated genes found variations in the microRNAs miR-17 and miR-30c-1, these patients were noncarriers of BRCA1 or BRCA2 mutations, lending the possibility that familial breast cancer may be caused by variation in these miRNAs. Members of the miR-30 family have been found to be highly expressed in heart cells.

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Stem-loop
      u a    uauu       u   aca         gugaaa 
5' gag g caga    guaaaca ccu   cucucagcu      a
   ||| | ||||    ||||||| |||   |||||||||       
3' uuc c gucu    cauuugu gga   gagggucga      g
      - -    cucu       c   --a         aagaau 
Get sequence
Deep sequencing
353030 reads, 2.09e+03 reads per million, 108 experiments
Confidence Annotation confidence: high
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Comments

miR-30c was cloned and mapped to chromosome 4 in reference [1] (MI0000547). A search of more recent mouse genome assemblies suggests the presence of a second locus encoding miR-30c on chromosome 1, represented by this entry.

Genome context
Coordinates (GRCm38) Overlapping transcripts
chr1: 23291701-23291784 [+]
sense
OTTMUST00000114071 ; RP23-28L24.7-001; exon 1
ENSMUST00000182303 ; Gm27028-001; exon 1
Database links

Mature sequence mmu-miR-30c-5p

Accession MIMAT0000514
Previous IDsmmu-miR-30c
Sequence

14 - 

uguaaacauccuacacucucagc

 - 36

Get sequence
Deep sequencing354310 reads, 92 experiments
Evidence experimental; cloned [1-4], Illumina [5-6]
Validated targets
Predicted targets

Mature sequence mmu-miR-30c-2-3p

Accession MIMAT0005438
Previous IDsmmu-miR-30c-2*
Sequence

54 - 

cugggagaaggcuguuuacucu

 - 75

Get sequence
Deep sequencing14992 reads, 88 experiments
Evidence experimental; cloned [4], Illumina [5-6]
Predicted targets

References

1
PMID:12007417 "Identification of tissue-specific microRNAs from mouse" Lagos-Quintana M, Rauhut R, Yalcin A, Meyer J, Lendeckel W, Tuschl T Curr Biol. 12:735-739(2002).
2
PMID:15538371 "A pancreatic islet-specific microRNA regulates insulin secretion" Poy MN, Eliasson L, Krutzfeldt J, Kuwajima S, Ma X, Macdonald PE, Pfeffer S, Tuschl T, Rajewsky N, Rorsman P, Stoffel M Nature. 432:226-230(2004).
3
4
PMID:17604727 "A mammalian microRNA expression atlas based on small RNA library sequencing" Landgraf P, Rusu M, Sheridan R, Sewer A, Iovino N, Aravin A, Pfeffer S, Rice A, Kamphorst AO, Landthaler M, Lin C, Socci ND, Hermida L, Fulci V, Chiaretti S, Foa R, Schliwka J, Fuchs U, Novosel A, Muller RU, Schermer B, Bissels U, Inman J, Phan Q, Chien M Cell. 129:1401-1414(2007).
5
PMID:20215419 "MicroRNA transcriptome in the newborn mouse ovaries determined by massive parallel sequencing" Ahn HW, Morin RD, Zhao H, Harris RA, Coarfa C, Chen ZJ, Milosavljevic A, Marra MA, Rajkovic A Mol Hum Reprod. 16:463-471(2010).
6
PMID:20413612 "Mammalian microRNAs: experimental evaluation of novel and previously annotated genes" Chiang HR, Schoenfeld LW, Ruby JG, Auyeung VC, Spies N, Baek D, Johnston WK, Russ C, Luo S, Babiarz JE, Blelloch R, Schroth GP, Nusbaum C, Bartel DP Genes Dev. 24:992-1009(2010).