Stem-loop sequence hsa-mir-195

AccessionMI0000489
Symbol HGNC:MIR195
DescriptionHomo sapiens miR-195 stem-loop
Gene family MIPF0000006; mir-15
Community annotation

This text is a summary paragraph taken from the Wikipedia entry entitled mir-15_microRNA_precursor_family. miRBase and Rfam are facilitating community annotation of microRNA families and entries in Wikipedia. Read more ...

The miR-15 microRNA precursor family is made up of small non-coding RNA genes that regulate gene expression. The family includes the related mir-15a and mir-15b sequences, as well as miR-16-1, miR-16-2, miR-195 and miR-497. These six highly conserved miRNAs are clustered on three separate chromosomes. In humans miR-15a and miR-16 are clustered within 0.5 kilobases at chromosome position 13q14. This region has been found to be the most commonly affected in chronic lymphocytic leukaemia (CLL), with deletions of the entire region in more than half of cases. Both miR-15a and miR-16 are thus frequently deleted or down-regulated in CLL samples with 13q14 deletions; occurring in more than two thirds of CLL cases. miR-15a/16-1 deletion has been shown to accelerate the proliferation of both human and mouse B-cells through modulation of the expression of genes controlling cell cycle progression. Studies have found the miR-15a/16-1 microRNA cluster to function as a tumour suppressor, with the oncogene BCL2 as its target. Specifically, miR-15a/16-1 downregulates BCL2 expression and is itself deleted or downregulated in tumour cells. There is a marked increase in BCL2 levels observed in advanced prostate tumour cases, which is inversely correlated with miR-15a/16-1 expression (and so corresponds to a decrease in miR-15a/16-1 levels). Inhibition of cell proliferation by the miR-15a/16-1 cluster occurs in both lymphoid and non-lymphoid tissue. The miR-15a/16-1 cluster has further been found to be highly expressed in CD5+ cells, therefore hinting at an important role of miR-15/16 in normal CD5+ B-cell homeostasis.

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Stem-loop
   a   u     g  cu          -a          caggga 
5'  gcu cccug cu  agcagcacag  aauauuggca      a
    ||| ||||| ||  ||||||||||  ||||||||||       
3'  ugg gggac ga  ucgucguguc  uuauaaccgu      g
   g   u     g  cc          gg          cugagc 
Get sequence
Deep sequencing
13777 reads, 3.34e+03 reads per million, 72 experiments
Confidence Annotation confidence: high
Feedback: Do you believe this miRNA is real?
Comments

This miRNA sequence is predicted based on homology to a verified miRNA from mouse [1], later verified in human [2].

Genome context
Coordinates (GRCh38) Overlapping transcripts
chr17: 7017615-7017701 [-]
sense
OTTHUMT00000220003 ; ASGR2-002; 5'UTR (exon 2)
OTTHUMT00000220002 ; ASGR2-001; 5'UTR (exon 2)
OTTHUMT00000439920 ; ASGR2-005; exon 2
OTTHUMT00000439921 ; ASGR2-006; exon 3
OTTHUMT00000439938 ; ASGR2-007; 5'UTR (exon 4)
ENST00000355035 ; ASGR2-002; 5'UTR (exon 2)
ENST00000254850 ; ASGR2-001; 5'UTR (exon 2)
ENST00000576487 ; ASGR2-005; exon 2
ENST00000380952 ; ASGR2-201; 5'UTR (exon 2)
ENST00000450034 ; ASGR2-006; exon 3
ENST00000574868 ; ASGR2-007; 5'UTR (exon 4)
Clustered miRNAs
< 10kb from hsa-mir-195
hsa-mir-497chr17: 7017911-7018022 [-]
hsa-mir-195chr17: 7017615-7017701 [-]
Database links

Mature sequence hsa-miR-195-5p

Accession MIMAT0000461
Previous IDshsa-miR-195
Sequence

15 - 

uagcagcacagaaauauuggc

 - 35

Get sequence
Deep sequencing734 reads, 44 experiments
Evidence experimental; cloned [2]
Validated targets
Predicted targets

Mature sequence hsa-miR-195-3p

Accession MIMAT0004615
Previous IDshsa-miR-195*
Sequence

53 - 

ccaauauuggcugugcugcucc

 - 74

Get sequence
Deep sequencing7 reads, 2 experiments
Evidence experimental; cloned [2]
Predicted targets

References

1
PMID:12554859 "New microRNAs from mouse and human" Lagos-Quintana M, Rauhut R, Meyer J, Borkhardt A, Tuschl T RNA. 9:175-179(2003).
2
PMID:17604727 "A mammalian microRNA expression atlas based on small RNA library sequencing" Landgraf P, Rusu M, Sheridan R, Sewer A, Iovino N, Aravin A, Pfeffer S, Rice A, Kamphorst AO, Landthaler M, Lin C, Socci ND, Hermida L, Fulci V, Chiaretti S, Foa R, Schliwka J, Fuchs U, Novosel A, Muller RU, Schermer B, Bissels U, Inman J, Phan Q, Chien M Cell. 129:1401-1414(2007).