Stem-loop sequence dme-mir-315

AccessionMI0000427 (change log)
DescriptionDrosophila melanogaster miR-315 stem-loop
Gene family MIPF0000141; mir-315
Literature search

5 open access papers mention dme-mir-315
(10 sentences)

Stem-loop
          auaa   ug          a       cuc    u 
5' cacuuau    uuu  auuguugcuc gaaagcc   auug u
   |||||||    |||  |||||||||| |||||||   |||| u
3' gugaaua    aaa  uaauaacgag cuuucgg   ugac a
          gacc   gu          -       --u    c 
Get sequence
Deep sequencing
297446 reads, 3.7e+03 reads per million, 46 experiments
Confidence Annotation confidence: high
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Comments

Lai et al. predicted this sequence based on conservation in D. pseudoobscura, but did not verify expression [2]. Aravin et al. describe identification and mapping of the ends of the excised sequence by cloning [1].

Genome context
Coordinates (Release_6; GCA_000001215.4) Overlapping transcripts
chr3L: 18860971-18861054 [+]
intergenic
Database links

Mature sequence dme-miR-315-5p

Accession MIMAT0000407
Previous IDsdme-miR-315
Sequence

12 - 

uuuugauuguugcucagaaagc

 - 33

Get sequence
Deep sequencing295294 reads, 46 experiments
Evidence experimental; cloned [1], 454 [3-4], Illumina [4]
Database links
Predicted targets

Mature sequence dme-miR-315-3p

Accession MIMAT0020841
Sequence

54 - 

cuuucgagcaauaauugaaacc

 - 75

Get sequence
Deep sequencing2149 reads, 41 experiments
Evidence not experimental
Database links

References

1
PMID:12919683 "The small RNA profile during Drosophila melanogaster development" Aravin AA, Lagos-Quintana M, Yalcin A, Zavolan M, Marks D, Snyder B, Gaasterland T, Meyer J, Tuschl T Dev Cell. 5:337-350(2003).
2
PMID:12844358 "Computational identification of Drosophila microRNA genes" Lai EC, Tomancak P, Williams RW, Rubin GM Genome Biol. 4:R42(2003).
3
PMID:17989254 "Evolution, biogenesis, expression, and target predictions of a substantially expanded set of Drosophila microRNAs" Ruby JG, Stark A, Johnston WK, Kellis M, Bartel DP, Lai EC Genome Res. 17:1850-1864(2007).
4
PMID:17989255 "Systematic discovery and characterization of fly microRNAs using 12 Drosophila genomes" Stark A, Kheradpour P, Parts L, Brennecke J, Hodges E, Hannon GJ, Kellis M Genome Res. 17:1865-1879(2007).