Stem-loop sequence dme-mir-309

Accession MI0000421
Description Drosophila melanogaster miR-309 stem-loop
Gene family MIPF0000140; mir-3
Stem-loop 
      c       c   uu    u   c aau 
auuaua gacaaac uug  cggu uug c   u
|||||| ||||||| |||  |||| ||| |   u
uaauau cuguuug aau  guca gac g   c
      c       a   gg    c   c aac
Get sequence
Deep sequencing
46572 reads, 38 experiments
Comments

miR-309 was identified and ends mapped by cloning. The sequence is localised to chromosome 2R.

Genome context
Coordinates (BDGP5.0) Overlapping transcripts
2R: 15549211-15549279 [-]
intergenic
Clustered miRNAs
< 10kb from dme-mir-309
dme-mir-309 2R: 15549211-15549279 [-]
dme-mir-3 2R: 15549100-15549168 [-]
dme-mir-286 2R: 15548927-15549026 [-]
dme-mir-4 2R: 15548794-15548874 [-]
dme-mir-5 2R: 15548663-15548731 [-]
dme-mir-6-1 2R: 15548508-15548589 [-]
dme-mir-6-2 2R: 15548374-15548447 [-]
dme-mir-6-3 2R: 15548221-15548300 [-]

Mature sequence dme-miR-309-5p

Accession MIMAT0020835
Sequence

7 -  

cgacaaaccuuguucgguuuug

 - 28

Get sequence
Deep sequencing 2205 reads, 21 experiments
Evidence not experimental
Predicted targets

Mature sequence dme-miR-309-3p

Accession MIMAT0000401
Previous IDs dme-miR-309
Sequence

44 -  

gcacuggguaaaguuuguccua

 - 65

Get sequence
Deep sequencing 44365 reads, 35 experiments
Evidence experimental; cloned [1], 454 [2-3], Solexa [3]
Predicted targets

References

1
PMID:12919683 "The small RNA profile during Drosophila melanogaster development" Aravin AA, Lagos-Quintana M, Yalcin A, Zavolan M, Marks D, Snyder B, Gaasterland T, Meyer J, Tuschl T Dev Cell. 5:337-350(2003).
2
PMID:17989254 "Evolution, biogenesis, expression, and target predictions of a substantially expanded set of Drosophila microRNAs" Ruby JG, Stark A, Johnston WK, Kellis M, Bartel DP, Lai EC Genome Res. 17:1850-1864(2007).
3
PMID:17989255 "Systematic discovery and characterization of fly microRNAs using 12 Drosophila genomes" Stark A, Kheradpour P, Parts L, Brennecke J, Hodges E, Hannon GJ, Kellis M Genome Res. 17:1865-1879(2007).