Stem-loop sequence dme-mir-307a

Accession

MI0000418 (change log)

Previous IDs

dme-mir-307

Description

Drosophila melanogaster miR-307a stem-loop

Gene family

MIPF0000293; mir-67

Literature search

6 open access papers mention dme-mir-307a
(51 sentences)

Stem-loop

   u        -   a         ccu  g       -u    uc 
5'  gucuugcu uug cucacucaa   gg ugugaug  uauu  g
    |||||||| ||| |||||||||   || |||||||  ||||  a
3'  caggacga agc gagugaguu   cc acacuac  augg  u
   a        u   -         ccu  a       cu    ua 

Deep sequencing

26774 reads, 786 reads per million, 49 experiments

Confidence

Annotation confidence: high

Feedback: Do you believe this miRNA is real?

Genome context

• Coordinates (Release_6; GCA_000001215.4): chr2R: 9621251-9621338 [-]
• Overlapping transcripts:

  sense

  FBtr0088501 ; Mmp2-RB; intron 3

  antisense

  FBtr0304171 ; mir-307b-RM; exon 1

Clustered miRNAs

< 10kb from dme-mir-307a

• dme-mir-307a: chr2R: 9621251-9621338 [-]
• dme-mir-307b: chr2R: 9621234-9621353 [+]

Database links

• RNAcentral:URS00000FFDD9_7227
• miRBase Tracker:MI0000418

Mature sequence dme-miR-307a-5p

• Accession: MIMAT0020832
• Sequence: 13 - acucacucaaccugggugugau - 34
• Deep sequencing: 4784 reads, 44 experiments
• Evidence: not experimental
• Database links:
  • RNAcentral:URS00003591F6_7227

Mature sequence dme-miR-307a-3p

• Accession: MIMAT0000398
• Previous IDs: dme-miR-307
• Sequence: 56 - ucacaaccuccuugagugagcga - 78
• Deep sequencing: 21965 reads, 49 experiments
• Evidence: experimental; cloned [1], 454 [2-3], Illumina [3]
• Database links:
  • RNAcentral:URS00000FBB5F_7227
• Predicted targets:
  • TargetScanFly:dme-miR-307
  • microrna.org:dme-miR-307a-3p

References

1. PMID:12919683 "The small RNA profile during Drosophila melanogaster development" Aravin AA, Lagos-Quintana M, Yalcin A, Zavolan M, Marks D, Snyder B, Gaasterland T, Meyer J, Tuschl T Dev Cell. 5:337-350(2003).
2. PMID:17989254 "Evolution, biogenesis, expression, and target predictions of a substantially expanded set of Drosophila microRNAs" Ruby JG, Stark A, Johnston WK, Kellis M, Bartel DP, Lai EC Genome Res. 17:1850-1864(2007).
3. PMID:17989255 "Systematic discovery and characterization of fly microRNAs using 12 Drosophila genomes" Stark A, Kheradpour P, Parts L, Brennecke J, Hodges E, Hannon GJ, Kellis M Genome Res. 17:1865-1879(2007).