Stem-loop sequence dme-mir-284

Accession MI0000369
Description Drosophila melanogaster miR-284 stem-loop
Gene family MIPF0000228; mir-284
Stem-loop 
----         c              a   uguagccugugagggcaag 
    guugcaguu cuggaauuaaguug cug                   g
    ||||||||| |||||||||||||| |||                   c
    cggcguuaa gaccuuaguucaac gac                   u
agcc         c              -   ugaaguccucguaauaagu
Get sequence
Deep sequencing
45791 reads, 46 experiments
Comments

This sequence is computationally predicted based on conservation in D. pseudoobscura. The precise 5' or 3' ends are unknown. Northern blotting confirmed that the strand containing the predicted miR is predominantly expressed. The sequence is localised to chromosome 3R.

Genome context
Coordinates (BDGP5.0) Overlapping transcripts
3R: 8377237-8377336 [-]
intergenic

Mature sequence dme-miR-284-5p

Accession MIMAT0020811
Sequence

10 -  

ccuggaauuaaguugacuguguagcc

 - 35

Get sequence
Deep sequencing 7951 reads, 40 experiments
Evidence not experimental
Predicted targets

Mature sequence dme-miR-284-3p

Accession MIMAT0000348
Previous IDs dme-miR-284
Sequence

64 -  

ugaagucagcaacuugauuccagcaauug

 - 92

Get sequence
Deep sequencing 37836 reads, 45 experiments
Evidence experimental; Northern [1], 454 [2-3], Solexa [3]
Predicted targets

References

1
PMID:12844358 "Computational identification of Drosophila microRNA genes" Lai EC, Tomancak P, Williams RW, Rubin GM Genome Biol. 4:R42(2003).
2
PMID:17989254 "Evolution, biogenesis, expression, and target predictions of a substantially expanded set of Drosophila microRNAs" Ruby JG, Stark A, Johnston WK, Kellis M, Bartel DP, Lai EC Genome Res. 17:1850-1864(2007).
3
PMID:17989255 "Systematic discovery and characterization of fly microRNAs using 12 Drosophila genomes" Stark A, Kheradpour P, Parts L, Brennecke J, Hodges E, Hannon GJ, Kellis M Genome Res. 17:1865-1879(2007).