Stem-loop sequence hsa-mir-200b

Accession

MI0000342 (change log)

Symbol

HGNC:MIR200B

Description

Homo sapiens miR-200b stem-loop

Gene family

MIPF0000019; mir-8

Literature search

680 open access papers mention hsa-mir-200b
(4422 sentences)

Stem-loop

   cca  uc    ag    g c    -    ug     c      u  ag 
5'    gc  gggc  ccgu g cauc uuac  ggcag auugga gg  u
      ||  ||||  |||| | |||| ||||  ||||| |||||| ||  c
3'    cg  cccg  ggcg c guag aaug  ccguc uaaucu cu  a
   gca  -u    -a    g a    u    gu     a      -  gg 

Deep sequencing

1472281 reads, 3.83e+03 reads per million, 156 experiments

Confidence

Annotation confidence: high

Feedback: Do you believe this miRNA is real?

Comments

The mature sequence shown here represents the most commonly cloned form from large-scale cloning studies [4].

Genome context

• Coordinates (GRCh38; GCA_000001405.15): chr1: 1167104-1167198 [+]
• Overlapping transcripts:

  antisense

  OTTHUMT00000005064 ; SDF4-001; intron 1

  OTTHUMT00000005065 ; SDF4-002; intron 1

  OTTHUMT00000005070 ; SDF4-007; intron 1

  OTTHUMT00000005068 ; SDF4-005; intron 1

  ENST00000360001 ; SDF4-001; intron 1

  ENST00000465727 ; SDF4-002; intron 1

  ENST00000263741 ; SDF4-007; intron 1

  ENST00000459994 ; SDF4-005; intron 1

  ENST00000545427 ; SDF4-201; intron 1

Clustered miRNAs

< 10kb from hsa-mir-200b

• hsa-mir-200b: chr1: 1167104-1167198 [+]
• hsa-mir-200a: chr1: 1167863-1167952 [+]
• hsa-mir-429: chr1: 1169005-1169087 [+]

Database links

• EntrezGene:MIR200B
• HGNC:MIR200B
• RNAcentral:URS000075C8DF_9606
• miRBase Tracker:MI0000342

Mature sequence hsa-miR-200b-5p

• Accession: MIMAT0004571
• Previous IDs: hsa-miR-200b*
• Sequence: 21 - caucuuacugggcagcauugga - 42
• Deep sequencing: 26658 reads, 120 experiments
• Evidence: experimental; cloned [4]
• Database links:
  • RNAcentral:URS000012A1DD_9606
• Predicted targets:
  • TargetMiner:hsa-miR-200b-5p
  • TargetScanVert:hsa-miR-200b-5p
  • miRDB:hsa-miR-200b-5p
  • microrna.org:hsa-miR-200b-5p

Mature sequence hsa-miR-200b-3p

• Accession: MIMAT0000318
• Previous IDs: hsa-miR-200b
• Sequence: 57 - uaauacugccugguaaugauga - 78
• Deep sequencing: 1445603 reads, 157 experiments
• Evidence: experimental; Northern [1], cloned [2-5]
• Database links:
  • RNAcentral:URS000014D9C1_9606
• Predicted targets:
  • TargetMiner:hsa-miR-200b-3p
  • TargetScanVert:hsa-miR-200b-3p
  • miRDB:hsa-miR-200b-3p
  • microrna.org:hsa-miR-200b-3p

References

1. PMID:12769849 "Computational and experimental identification of C. elegans microRNAs" Grad Y, Aach J, Hayes GD, Reinhart BJ, Church GM, Ruvkun G, Kim J Mol Cell. 11:1253-1263(2003).
2. PMID:14573789 "Reduced accumulation of specific microRNAs in colorectal neoplasia" Michael MZ, O' Connor SM, van Holst Pellekaan NG, Young GP, James RJ Mol Cancer Res. 1:882-891(2003).
3. PMID:15891114 "Clustering and conservation patterns of human microRNAs" Altuvia Y, Landgraf P, Lithwick G, Elefant N, Pfeffer S, Aravin A, Brownstein MJ, Tuschl T, Margalit H Nucleic Acids Res. 33:2697-2706(2005).
4. PMID:17604727 "A mammalian microRNA expression atlas based on small RNA library sequencing" Landgraf P, Rusu M, Sheridan R, Sewer A, Iovino N, Aravin A, Pfeffer S, Rice A, Kamphorst AO, Landthaler M, Lin C, Socci ND, Hermida L, Fulci V, Chiaretti S, Foa R, Schliwka J, Fuchs U, Novosel A, Muller RU, Schermer B, Bissels U, Inman J, Phan Q, Chien M Cell. 129:1401-1414(2007).
5. PMID:17616659 "Patterns of known and novel small RNAs in human cervical cancer" Lui WO, Pourmand N, Patterson BK, Fire A Cancer Res. 67:6031-6043(2007).