Stem-loop sequence hsa-mir-200b

Symbol HGNC:MIR200B
DescriptionHomo sapiens miR-200b stem-loop
Gene family MIPF0000019; mir-8
Community annotation

This text is a summary paragraph taken from the Wikipedia entry entitled mir-8/mir-141/mir-200_microRNA_precursor_family. miRBase and Rfam are facilitating community annotation of microRNA families and entries in Wikipedia. Read more ...

The miR-8 microRNA precursor (homologous to miR-141, miR-200, miR-236), is a short non-coding RNA gene involved in gene regulation. miR-8 in Drosophila melanogaster is expressed from the 3' arm of related precursor hairpins (represented here), along with miR-200, miR-236, miR-429 and human and mouse homolog miR-141. Members of this precursor family have now been predicted or experimentally confirmed in a wide range of species. The bounds of the precursors are predicted based on conservation and base pairing and are not generally known. The miR-200 family is highly conserved in bilaterian animals, with miR-8 as the sole homolog in Drosophila. This species has accordingly been used heavily in work looking to uncover the biological function of the miR-200 family. miR-8 has been observed at all developmental stages of the embryo, with a complete absence from cultured S2 cells of D. melanogaster. Its expression has been seen to be strongly upregulated in larvae and this expression then sustained through to adulthood.

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   cca  uc    ag    g c    -    ug     c      u  ag 
5'    gc  gggc  ccgu g cauc uuac  ggcag auugga gg  u
      ||  ||||  |||| | |||| ||||  ||||| |||||| ||  c
3'    cg  cccg  ggcg c guag aaug  ccguc uaaucu cu  a
   gca  -u    -a    g a    u    gu     a      -  gg 
Get sequence
Deep sequencing
20016 reads, 6.62e+03 reads per million, 60 experiments
Confidence Annotation confidence: high
Feedback: Do you believe this miRNA is real?

The mature sequence shown here represents the most commonly cloned form from large-scale cloning studies [4].

Genome context
Coordinates (GRCh38) Overlapping transcripts
chr1: 1167104-1167198 [+]
OTTHUMT00000005064 ; SDF4-001; intron 1
OTTHUMT00000005065 ; SDF4-002; intron 1
OTTHUMT00000005070 ; SDF4-007; intron 1
OTTHUMT00000005068 ; SDF4-005; intron 1
ENST00000360001 ; SDF4-001; intron 1
ENST00000465727 ; SDF4-002; intron 1
ENST00000263741 ; SDF4-007; intron 1
ENST00000459994 ; SDF4-005; intron 1
ENST00000545427 ; SDF4-201; intron 1
Clustered miRNAs
< 10kb from hsa-mir-200b
hsa-mir-200bchr1: 1167104-1167198 [+]
hsa-mir-200achr1: 1167863-1167952 [+]
hsa-mir-429chr1: 1169005-1169087 [+]
Database links

Mature sequence hsa-miR-200b-5p

Accession MIMAT0004571
Previous IDshsa-miR-200b*

21 - 


 - 42

Get sequence
Deep sequencing284 reads, 35 experiments
Evidence experimental; cloned [4]
Predicted targets

Mature sequence hsa-miR-200b-3p

Accession MIMAT0000318
Previous IDshsa-miR-200b

57 - 


 - 78

Get sequence
Deep sequencing597 reads, 45 experiments
Evidence experimental; Northern [1], cloned [2-5]
Validated targets
Predicted targets


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PMID:14573789 "Reduced accumulation of specific microRNAs in colorectal neoplasia" Michael MZ, O' Connor SM, van Holst Pellekaan NG, Young GP, James RJ Mol Cancer Res. 1:882-891(2003).
PMID:15891114 "Clustering and conservation patterns of human microRNAs" Altuvia Y, Landgraf P, Lithwick G, Elefant N, Pfeffer S, Aravin A, Brownstein MJ, Tuschl T, Margalit H Nucleic Acids Res. 33:2697-2706(2005).
PMID:17604727 "A mammalian microRNA expression atlas based on small RNA library sequencing" Landgraf P, Rusu M, Sheridan R, Sewer A, Iovino N, Aravin A, Pfeffer S, Rice A, Kamphorst AO, Landthaler M, Lin C, Socci ND, Hermida L, Fulci V, Chiaretti S, Foa R, Schliwka J, Fuchs U, Novosel A, Muller RU, Schermer B, Bissels U, Inman J, Phan Q, Chien M Cell. 129:1401-1414(2007).
PMID:17616659 "Patterns of known and novel small RNAs in human cervical cancer" Lui WO, Pourmand N, Patterson BK, Fire A Cancer Res. 67:6031-6043(2007).