Stem-loop sequence hsa-mir-196a-2

AccessionMI0000279 (change log)
Previous IDshsa-mir-196-2
Symbol HGNC:MIR196A2
DescriptionHomo sapiens miR-196a-2 stem-loop
Gene family MIPF0000031; mir-196
Community annotation

This text is a summary paragraph taken from the Wikipedia entry entitled mir-196_microRNA_precursor_family. miRBase and Rfam are facilitating community annotation of microRNA families and entries in Wikipedia. Read more ...

miR-196 is a non-coding RNA called a microRNA that has been shown to be expressed in human (MI0000238, MI0000279) and mouse (MI0000552, MI0000553). miR-196 appears to be a vertebrate specific microRNA and has now been predicted or experimentally confirmed in a wide range of vertebrate species (MIPF0000031). In many species the miRNA appears to be expressed from intergenic regions in HOX gene clusters. The hairpin precursors are predicted based on base pairing and cross-species conservation—their extents are not known. In this case the mature sequence is excised from the 5' arm of the hairpin. It has been suggested that a rare SNP (rs11614913) that overlaps hsa-mir-196a2 has been found to be associated with non-small cell lung carcinoma.

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   -ug    - -uc       --c                  a          a ug   
5'    cucg c   agcugau   uguggcuuagguaguuuc uguuguuggg u  ag 
      |||| |   |||||||   |||||||||||||||||| |||||||||| |  | u
3'    gagc g   uuggcug   acauugaguccgucaaag acaacggcuc a  uu 
   cgg    u cuu       acu                  a          a gu   
Get sequence
Deep sequencing
376900 reads, 2.3e+03 reads per million, 74 experiments
Confidence Annotation confidence: high
Feedback: Do you believe this miRNA is real?

This human miRNA was predicted by computational methods using conservation with mouse and Fugu rubripes sequences [1]. Expression of the excised miR has been validated in zebrafish, and the ends mapped by cloning. miR-196a was later validated in human [3,4]. Yekta et al. report that miR-196 miRNAs are expressed from HOX gene clusters in mammals, and that HOX genes in these clusters are targets of miR-196. Indeed, HOXB8 mRNA was shown to be a natural target for miR-196-directed cleavage through a perfectly complementary miR-target site. Other HOX genes have imperfect miR-196 complementary sites indicative of regulation by translational repression [2]. The mature sequence shown here represents the most commonly cloned form from large-scale cloning studies [4].

Genome context
Coordinates (GRCh38; GCA_000001405.15) Overlapping transcripts
chr12: 53991738-53991847 [+]
OTTHUMT00000460684 ; RP11-793H13.10-001; intron 2
OTTHUMT00000406302 ; ATF7-007; intron 2
OTTHUMT00000405834 ; ATF7-001; intron 2
OTTHUMT00000460675 ; ATF7-006; intron 2
OTTHUMT00000460676 ; ATF7-010; intron 2
OTTHUMT00000405836 ; ATF7-005; intron 2
OTTHUMT00000460686 ; ATF7-011; intron 2
OTTHUMT00000460677 ; ATF7-009; intron 2
OTTHUMT00000405837 ; ATF7-002; intron 2
ENST00000328463 ; ATF7-201; intron 1
ENST00000591834 ; RP11-793H13.10-001; intron 2
ENST00000548446 ; ATF7-007; intron 2
ENST00000420353 ; ATF7-001; intron 2
ENST00000456903 ; ATF7-006; intron 2
ENST00000588232 ; ATF7-010; intron 2
ENST00000551480 ; ATF7-005; intron 2
ENST00000588078 ; ATF7-011; intron 2
ENST00000548118 ; ATF7-009; intron 2
ENST00000591397 ; ATF7-002; intron 2
ENST00000415113 ; ATF7-202; intron 2
Database links

Mature sequence hsa-miR-196a-5p

Accession MIMAT0000226
Previous IDshsa-miR-196a

25 - 


 - 46

Get sequence
Deep sequencing745468 reads, 73 experiments
Evidence experimental; cloned [3-5]
Database links
Predicted targets

Mature sequence hsa-miR-196a-3p

Accession MIMAT0004562
Previous IDshsa-miR-196a*

62 - 


 - 83

Get sequence
Deep sequencing2997 reads, 42 experiments
Evidence experimental; cloned [4]
Database links
Predicted targets


PMID:12624257 "Vertebrate microRNA genes" Lim LP, Glasner ME, Yekta S, Burge CB, Bartel DP Science. 299:1540(2003).
PMID:15105502 "MicroRNA-directed cleavage of HOXB8 mRNA" Yekta S, Shih IH, Bartel DP Science. 304:594-596(2004).
PMID:12554859 "New microRNAs from mouse and human" Lagos-Quintana M, Rauhut R, Meyer J, Borkhardt A, Tuschl T RNA. 9:175-179(2003).
PMID:17604727 "A mammalian microRNA expression atlas based on small RNA library sequencing" Landgraf P, Rusu M, Sheridan R, Sewer A, Iovino N, Aravin A, Pfeffer S, Rice A, Kamphorst AO, Landthaler M, Lin C, Socci ND, Hermida L, Fulci V, Chiaretti S, Foa R, Schliwka J, Fuchs U, Novosel A, Muller RU, Schermer B, Bissels U, Inman J, Phan Q, Chien M Cell. 129:1401-1414(2007).
PMID:17616659 "Patterns of known and novel small RNAs in human cervical cancer" Lui WO, Pourmand N, Patterson BK, Fire A Cancer Res. 67:6031-6043(2007).