Stem-loop sequence mmu-mir-185

Accession

MI0000227

Symbol

MGI:Mir185

Description

Mus musculus miR-185 stem-loop

Gene family

MIPF0000202; mir-185

Stem-loop

      ug   a     g         au  uc 
agggau  gag gaaag caguuccug  gg  c
||||||  ||| ||||| |||||||||  ||   
uuccug  cuc cuuuc gucggggac  cc  c
      gu   -     g         --  uc 

Deep sequencing

646370 reads, 96 experiments

Comments

The mature sequence shown here represents the most commonly cloned form from large-scale cloning studies [2].

Genome context

• Coordinates (GRCm38): chr16: 18327401-18327465 [-]
• Overlapping transcripts:

  sense

  OTTMUST00000064223 ; RP23-47O21.5-003; intron 1

  OTTMUST00000064868 ; RP23-47O21.5-010; intron 1

  OTTMUST00000064224 ; RP23-47O21.5-004; intron 1

  OTTMUST00000064221 ; RP23-47O21.5-001; intron 1

  ENSMUST00000083530 ; Mir185-201; exon 1

  ENSMUST00000134385 ; D16H22S680E-003; intron 1

  ENSMUST00000130752 ; D16H22S680E-010; intron 1

  ENSMUST00000125287 ; D16H22S680E-004; intron 1

  ENSMUST00000115628 ; D16H22S680E-001; intron 1

Database links

• EMBL: AJ560727;MMU560727
• ENTREZGENE: 387180;Mir185
• MGI: 2676849;Mir185

Mature sequence mmu-miR-185-5p

• Accession: MIMAT0000214
• Previous IDs: mmu-miR-185
• Sequence: 7 - uggagagaaaggcaguuccuga - 28
• Deep sequencing: 525467 reads, 81 experiments
• Evidence: experimental; cloned [1-2], Solexa [3-4]
• Validated targets:
  • TARBASE:mmu-miR-185-5p
• Predicted targets:
  • DIANA-MICROT:mmu-miR-185-5p
  • MICRORNA.ORG:mmu-miR-185-5p
  • MIRDB:mmu-miR-185-5p
  • RNA22-MMU:mmu-miR-185-5p
  • TARGETSCAN-VERT:mmu-miR-185

Mature sequence mmu-miR-185-3p

• Accession: MIMAT0016996
• Previous IDs: mmu-miR-185*
• Sequence: 41 - aggggcuggcuuuccucuggu - 61
• Deep sequencing: 1444 reads, 76 experiments
• Evidence: experimental; Solexa [4]
• Predicted targets:
  • DIANA-MICROT:mmu-miR-185-3p
  • MIRDB:mmu-miR-185-3p
  • RNA22-MMU:mmu-miR-185-3p

References

1. PMID:12554859 "New microRNAs from mouse and human" Lagos-Quintana M, Rauhut R, Meyer J, Borkhardt A, Tuschl T RNA. 9:175-179(2003).
2. PMID:17604727 "A mammalian microRNA expression atlas based on small RNA library sequencing" Landgraf P, Rusu M, Sheridan R, Sewer A, Iovino N, Aravin A, Pfeffer S, Rice A, Kamphorst AO, Landthaler M, Lin C, Socci ND, Hermida L, Fulci V, Chiaretti S, Foa R, Schliwka J, Fuchs U, Novosel A, Muller RU, Schermer B, Bissels U, Inman J, Phan Q, Chien M Cell. 129:1401-1414(2007).
3. PMID:20215419 "MicroRNA transcriptome in the newborn mouse ovaries determined by massive parallel sequencing" Ahn HW, Morin RD, Zhao H, Harris RA, Coarfa C, Chen ZJ, Milosavljevic A, Marra MA, Rajkovic A Mol Hum Reprod. 16:463-471(2010).
4. PMID:20413612 "Mammalian microRNAs: experimental evaluation of novel and previously annotated genes" Chiang HR, Schoenfeld LW, Ruby JG, Auyeung VC, Spies N, Baek D, Johnston WK, Russ C, Luo S, Babiarz JE, Blelloch R, Schroth GP, Nusbaum C, Bartel DP Genes Dev. 24:992-1009(2010).