Stem-loop sequence mmu-mir-99b

Accession

MI0000147

Symbol

MGI:Mir99b

Description

Mus musculus miR-99b stem-loop

Gene family

MIPF0000025; mir-99

Stem-loop

     cc         ac   c    c - -   c 
ggcac  acccguaga  cga cuug g g ggc u
|||||  |||||||||  ||| |||| | | |||  
cugug  ugggugucu  gcu gaac c c ccg u
     cc         gu   c    a a g   c 

Deep sequencing

151831 reads, 97 experiments

Comments

Expression of this miRNA in mouse was independently verified in the brain [1], embryonic stem cells [2] and testes [3].

Genome context

• Coordinates (GRCm38): chr17: 17830188-17830257 [+]
• Overlapping transcripts:

  intergenic

Clustered miRNAs

< 10kb from mmu-mir-99b

• mmu-mir-99b: chr17: 17830188-17830257 [+]
• mmu-let-7e: chr17: 17830352-17830444 [+]
• mmu-mir-125a: chr17: 17830812-17830879 [+]

Database links

• EMBL: AJ459729;MMU459729
• ENTREZGENE: 387230;Mir99b
• MGI: 2676913;Mir99b

Mature sequence mmu-miR-99b-5p

• Accession: MIMAT0000132
• Previous IDs: mmu-miR-99b
• Sequence: 7 - cacccguagaaccgaccuugcg - 28
• Deep sequencing: 126959 reads, 82 experiments
• Evidence: experimental; cloned [1-4], Solexa [5-6]
• Predicted targets:
  • DIANA-MICROT:mmu-miR-99b-5p
  • MICRORNA.ORG:mmu-miR-99b-5p
  • MIRDB:mmu-miR-99b-5p
  • RNA22-MMU:mmu-miR-99b-5p
  • TARGETSCAN-VERT:mmu-miR-99b

Mature sequence mmu-miR-99b-3p

• Accession: MIMAT0004525
• Previous IDs: mmu-miR-99b*
• Sequence: 45 - caagcucgugucuguggguccg - 66
• Deep sequencing: 4942 reads, 69 experiments
• Evidence: experimental; cloned [4], Solexa [5-6]
• Predicted targets:
  • DIANA-MICROT:mmu-miR-99b-3p
  • MICRORNA.ORG:mmu-miR-99b-3p
  • MIRDB:mmu-miR-99b-3p
  • RNA22-MMU:mmu-miR-99b-3p

References

1. PMID:12007417 "Identification of tissue-specific microRNAs from mouse" Lagos-Quintana M, Rauhut R, Yalcin A, Meyer J, Lendeckel W, Tuschl T Curr Biol. 12:735-739(2002).
2. PMID:12919684 "Embryonic stem cell-specific MicroRNAs" Houbaviy HB, Murray MF, Sharp PA Dev Cell. 5:351-358(2003).
3. PMID:16766679 "Identification and characterization of two novel classes of small RNAs in the mouse germline: retrotransposon-derived siRNAs in oocytes and germline small RNAs in testes" Watanabe T, Takeda A, Tsukiyama T, Mise K, Okuno T, Sasaki H, Minami N, Imai H Genes Dev. 20:1732-1743(2006).
4. PMID:17604727 "A mammalian microRNA expression atlas based on small RNA library sequencing" Landgraf P, Rusu M, Sheridan R, Sewer A, Iovino N, Aravin A, Pfeffer S, Rice A, Kamphorst AO, Landthaler M, Lin C, Socci ND, Hermida L, Fulci V, Chiaretti S, Foa R, Schliwka J, Fuchs U, Novosel A, Muller RU, Schermer B, Bissels U, Inman J, Phan Q, Chien M Cell. 129:1401-1414(2007).
5. PMID:20215419 "MicroRNA transcriptome in the newborn mouse ovaries determined by massive parallel sequencing" Ahn HW, Morin RD, Zhao H, Harris RA, Coarfa C, Chen ZJ, Milosavljevic A, Marra MA, Rajkovic A Mol Hum Reprod. 16:463-471(2010).
6. PMID:20413612 "Mammalian microRNAs: experimental evaluation of novel and previously annotated genes" Chiang HR, Schoenfeld LW, Ruby JG, Auyeung VC, Spies N, Baek D, Johnston WK, Russ C, Luo S, Babiarz JE, Blelloch R, Schroth GP, Nusbaum C, Bartel DP Genes Dev. 24:992-1009(2010).