Stem-loop sequence hsa-let-7e

AccessionMI0000066
Previous IDshsa-let-7eL
Symbol HGNC:MIRLET7E
DescriptionHomo sapiens let-7e stem-loop
Gene family MIPF0000002; let-7
Community annotation

This text is a summary paragraph taken from the Wikipedia entry entitled let-7_microRNA_precursor. miRBase and Rfam are facilitating community annotation of microRNA families and entries in Wikipedia. Read more ...

The Let-7 microRNA precursor was identified from a study of developmental timing in C. elegans, and was later shown to be part of a much larger class of non-coding RNAs termed microRNAs. miR-98 microRNA precursor from human is a let-7 family member. Let-7 miRNAs have now been predicted or experimentally confirmed in a wide range of species (MIPF0000002). miRNAs are initially transcribed in long transcripts (up to several hundred nucleotides) called primary miRNAs (pri-miRNAs), which are processed in the nucleus by Drosha and Pasha to hairpin structures of about ~70 nucleotide. These precursors (pre-miRNAs) are exported to the cytoplasm by exportin5, where they are subsequently processed by the enzyme Dicer to a ~22 nucleotide mature miRNA. The involvement of Dicer in miRNA processing demonstrates a relationship with the phenomenon of RNA interference.

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Stem-loop
     c   cu   g                u  ----gga  a 
5' cc ggg  gag uaggagguuguauagu ga       gg c
   || |||  ||| |||||||||||||||| ||       ||  
3' gg ccc  uuc auccuccggcauauca cu       cc a
     a   cu   g                -  agaggaa  c 
Get sequence
Deep sequencing
178522 reads, 1.2e+04 reads per million, 76 experiments
Confidence Annotation confidence: high
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Comments

The mature sequence shown here represents the most commonly cloned form from large-scale cloning studies [3].

Genome context
Coordinates (GRCh38) Overlapping transcripts
chr19: 51692786-51692864 [+]
sense
OTTHUMT00000465190 ; SIGLEC20P-001; intron 6
ENST00000595243 ; SIGLEC20P-001; intron 6
Clustered miRNAs
< 10kb from hsa-let-7e
hsa-mir-99bchr19: 51692612-51692681 [+]
hsa-let-7echr19: 51692786-51692864 [+]
hsa-mir-125achr19: 51693254-51693339 [+]
Database links

Mature sequence hsa-let-7e-5p

Accession MIMAT0000066
Previous IDshsa-let-7e
Sequence

8 - 

ugagguaggagguuguauaguu

 - 29

Get sequence
Deep sequencing1795 reads, 65 experiments
Evidence experimental; cloned [1-4], Northern [1], Illumina [5]
Validated targets
Predicted targets

Mature sequence hsa-let-7e-3p

Accession MIMAT0004485
Previous IDshsa-let-7e*
Sequence

53 - 

cuauacggccuccuagcuuucc

 - 74

Get sequence
Deep sequencing149 reads, 30 experiments
Evidence experimental; cloned [3]
Validated targets
Predicted targets

References

1
PMID:11679670 "Identification of novel genes coding for small expressed RNAs" Lagos-Quintana M, Rauhut R, Lendeckel W, Tuschl T Science. 294:853-858(2001).
2
PMID:15325244 "Altered expression profiles of microRNAs during TPA-induced differentiation of HL-60 cells" Kasashima K, Nakamura Y, Kozu T Biochem Biophys Res Commun. 322:403-410(2004).
3
PMID:17604727 "A mammalian microRNA expression atlas based on small RNA library sequencing" Landgraf P, Rusu M, Sheridan R, Sewer A, Iovino N, Aravin A, Pfeffer S, Rice A, Kamphorst AO, Landthaler M, Lin C, Socci ND, Hermida L, Fulci V, Chiaretti S, Foa R, Schliwka J, Fuchs U, Novosel A, Muller RU, Schermer B, Bissels U, Inman J, Phan Q, Chien M Cell. 129:1401-1414(2007).
4
PMID:17616659 "Patterns of known and novel small RNAs in human cervical cancer" Lui WO, Pourmand N, Patterson BK, Fire A Cancer Res. 67:6031-6043(2007).
5