Stem-loop sequence cel-mir-80

Accession MI0000051
Description Caenorhabditis elegans miR-80/miR-227 stem-loop
Gene family MIPF0000258; mir-80
Stem-loop 
auggaca   -gu   c  a         --     u  g      ----c g 
       cuc   ucg uc gcuuucgac  augau cu aacaau     c c
       |||   ||| || |||||||||  ||||| || ||||||     |  
       gag   agu ag cgaaaguug  uacua ga uuguug     g a
--cuaua   acu   a  c         au     -  g      uaccc a
Get sequence
Deep sequencing
75582 reads, 6 experiments
Comments

mir-80 is conserved in C.briggsae (MI0000518 ) [1]. Reference [3] reports the identification of miR-227, which appears to be expressed from the 5' arm of the same precursor.

Genome context
Coordinates (WBcel215) Overlapping transcripts
III: 8865189-8865286 [-]
antisense
K01F9.6 ; K01F9.6; exon 1
K01F9.6 ; K01F9.6; exon 1
K01F9.6 ; K01F9.6; exon 1
Clustered miRNAs
< 10kb from cel-mir-80
cel-mir-90 III: 8873906-8874003 [-]
cel-mir-238 III: 8867247-8867344 [-]
cel-mir-80 III: 8865189-8865286 [-]
Database links

Mature sequence cel-miR-80-5p

Accession MIMAT0000052
Previous IDs cel-miR-227
Sequence

19 -  

agcuuucgacaugauucugaac

 - 40

Get sequence
Deep sequencing 150 reads, 5 experiments
Evidence experimental; cloned [3], Solexa [6], CLIPseq [7]
Validated targets
Predicted targets

Mature sequence cel-miR-80-3p

Accession MIMAT0000053
Previous IDs cel-miR-80
Sequence

61 -  

ugagaucauuaguugaaagccga

 - 83

Get sequence
Deep sequencing 75426 reads, 6 experiments
Evidence experimental; cloned [1-5], Northern [1], Solexa [6], CLIPseq [7]
Validated targets
Predicted targets

References

1
PMID:11679671 "An abundant class of tiny RNAs with probable regulatory roles in Caenorhabditis elegans" Lau NC, Lim LP, Weinstein EG, Bartel DP Science. 294:858-862(2001).
2
PMID:11679672 "An extensive class of small RNAs in Caenorhabditis elegans" Lee RC, Ambros V Science. 294:862-864(2001).
3
PMID:12747828 "MicroRNAs and other tiny endogenous RNAs in C. elegans" Ambros V, Lee RC, Lavanway A, Williams PT, Jewell D Curr Biol. 13:807-818(2003).
4
PMID:12672692 "The microRNAs of Caenorhabditis elegans" Lim LP, Lau NC, Weinstein EG, Abdelhakim A, Yekta S, Rhoades MW, Burge CB, Bartel DP Genes Dev. 17:991-1008(2003).
5
PMID:17174894 "Large-scale sequencing reveals 21U-RNAs and additional microRNAs and endogenous siRNAs in C. elegans" Ruby JG, Jan C, Player C, Axtell MJ, Lee W, Nusbaum C, Ge H, Bartel DP Cell. 127:1193-1207(2006).
6
PMID:19460142 "Dynamic expression of small non-coding RNAs, including novel microRNAs and piRNAs/21U-RNAs, during Caenorhabditis elegans development" Kato M, de Lencastre A, Pincus Z, Slack FJ Genome Biol. 10:R54(2009).
7
PMID:20062054 "Comprehensive discovery of endogenous Argonaute binding sites in Caenorhabditis elegans" Zisoulis DG, Lovci MT, Wilbert ML, Hutt KR, Liang TY, Pasquinelli AE, Yeo GW Nat Struct Mol Biol. 17:173-179(2010).