Deep sequencing reads for stem-loop sequence MI0000326

Stem-loop ID

cel-mir-250

Reads

                 cel-miR-250-5p                           cel-miR-250-3p                              Count     RPM (mean number of reads per million)
.................CCUUCAGUUGCCUCGUGAUCCG............................................................  21        11.9
.................CCUUCAGUUGCCUCGUGAUCC.............................................................  6         3.56
.................CCUUCAGUUGCCUCGUGAUC..............................................................  1         1.06
...........................................................AAUCACAGUCAACUGUUGGCA...................  359       487
...........................................................AAUCACAGUCAACUGUUGGC....................  333       445
...........................................................AAUCACAGUCAACUGUUGGCAU..................  106       153
...........................................................AAUCACAGUCAACUGUUGG.....................  46        73.2
...........................................................AAUCACAGUCAACUGUUG......................  11        24.9
...........................................................AAUCACAGUCAACUGUU.......................  5         8.01
...........................................................AAUCACAGUCAACUGUUGGCAUG.................  1         1.25
............................................................AUCACAGUCAACUGUUGGCA...................  18        29.6
............................................................AUCACAGUCAACUGUUGG.....................  4         3.89
............................................................AUCACAGUCAACUGUUGGC....................  1         1.25
............................................................AUCACAGUCAACUGUUGGCAU..................  1         0.463
.............................................................UCACAGUCAACUGUUGGCA...................  3         2.5
.............................................................UCACAGUCAACUGUUGGCAU..................  2         0.927
..............................................................CACAGUCAACUGUUGGCA...................  2         2.12
..............................................................CACAGUCAACUGUUGGC....................  2         3.15
...............................................................ACAGUCAACUGUUGGCA...................  2         3.8
AGGGUCUUCGGACCACGCCUUCAGUUGCCUCGUGAUCCGCCAAACACAAUAAAUGGACGAAUCACAGUCAACUGUUGGCAUGGUGCUCGUACCCAUUUU
.((((....(.((((.(((..((((((.((.(((((.(((((...........))).)).))))))).))))))..))).)))).)....))))..... (-38.50)

Filter reads

by experiment

Accession	Read count	Tissue	Link	Comment	Reference
ER0000000024	424	whole body	GEO : GSM378784		[ de Wit E et al.]
ER0000000203	63	adult	GEO : GSM604032	strain: N2 wild-type, age: Day 0	[ de Lencastre A et al.]
ER0000000204	146	adult	GEO : GSM604033	strain: N2 wild-type, age: Day 10	[ de Lencastre A et al.]
ER0000000205	139	adult	GEO : GSM604034	strain: daf-2(e1379) mutant, age: Day 0	[ de Lencastre A et al.]
ER0000000206	140	adult	GEO : GSM604035	strain: daf-2(e1379) mutant, age: Day 10	[ de Lencastre A et al.]
ER0000000212	185	whole body	GEO : GSM139137	mixed-stage	[ Ruby JG et al.]

References

1. PMID:17174894 "Large-scale sequencing reveals 21U-RNAs and additional microRNAs and endogenous siRNAs in C. elegans" Ruby JG, Jan C, Player C, Axtell MJ, Lee W, Nusbaum C, Ge H, Bartel DP Cell. 127:1193-1207(2006).
2. PMID:19755563 "Repertoire and evolution of miRNA genes in four divergent nematode species" de Wit E, Linsen SE, Cuppen E, Berezikov E Genome Res. 19:2064-2074(2009).
3. PMID:21129974 "MicroRNAs both promote and antagonize longevity in C. elegans" de Lencastre A, Pincus Z, Zhou K, Kato M, Lee SS, Slack FJ Curr Biol. 20:2159-2168(2010).